geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of the small heat shock protein Hsp27 gene in Chironomus riparius (Diptera) and its expression profile in response to temperature changes and xenobiotic exposures


, : Characterization of the small heat shock protein Hsp27 gene in Chironomus riparius (Diptera) and its expression profile in response to temperature changes and xenobiotic exposures. Cell Stress & Chaperones 19(4): 529-540

Small heat shock proteins constitute the most diverse and least conserved group within the large family of heat shock proteins, which play a crucial role in cell response to environmental insults. Chironomus riparius larvae are widely used in environmental research for testing pollutant toxicity in sediments and freshwater environments. Different genes, such as Hsp70, Hsc70, Hsp90, and Hsp40, have been identified in this species as sensitive biomarkers for xenobiotics, but small Hsps genes remain largely unknown. In this study, the Hsp27 has been characterized in C. riparius and its transcriptional response evaluated under several environmental stimuli. The Hsp27 gene was mapped by FISH on polytene chromosomes at region I-C4 and was found to encode a 195 aa protein, which contains an α-crystallin domain bounded by three conserved regions. This protein shows homology with Drosophila melanogaster HSP27, Ceratitis capitata HSP27, and Sarcophaga crassipalpis HSP25. Real-time reverse transcriptase-polymerase chain reaction analysis showed that heat shock (35 °C) and cadmium dramatically upregulate this gene. Moreover, exposures to triclosan and bisphenol A were able to significantly increase mRNA levels. However, neither nonylphenol nor tributyltin altered Hsp27 gene expression. The transcriptional activity of Hsp27 gene was modulated during cold stress. Interestingly, cold shock (4 °C) significantly reduced Hsp27 transcripts, but this gene was significantly overexpressed during the recovery time at the normal growing temperature. These results show that the Hsp27 gene is sensitive to different environmental stimuli, including endocrine-disrupting pollutants, suggesting its potential as a suitable biomarker for ecotoxicological studies in aquatic systems.

US$19.90

PMID: 24297481

DOI: 10.1007/s12192-013-0479-y


Other references

Tuz, As, 1978: Inheritance of anthocyanin colour of fruits in Pyrus domestica Medic. Genetika 14 (2) 223-229

Arfeux, M.; Artier, M.C.; Laluc, G., 1996: Thoracoscopy. Preparations in the operating room. Soins. Chirurgie: 14-18

Marco, J.; Lee, W.S.; Hoffman, L.; Honrubia, V., 1990: Efferent vestibular neurons in the chinchilla. Advances in Oto-Rhino-Laryngology 45: 82-93

Olsen, O., 1981: Flowering bamboos. A review of experience with growing bamboos outdoors in Denmark since they were first introduced in the 1920s. Flowering was observed in a number of species at different times since 1960.

Tealdo, A., 1992: Agriculture: limits of interchange and import substitution. Structural adjustment policies usually have in common the goal of improving trade relations with other countries and boosting exports. The paper sets out to examine the efficiency of such policies in relation to the agricultural sector within the...

Subhani, A.Rauf.; Malik, A.Saeed.; Kamel, N.; Saad, N.; Nandagopal, D.Nanda., 2016: Experimental evidence for the effects of the Demand-Control model on the cognitive arousal: An EEG based study. The Demand-Control (DC) model has been extensively researched to find the imbalance of demand and control that cause work-related stress. Past research has been exclusively dedicated to evaluate the impact of this model on employees' well-bei...

Maeda, A.; Matsuda, F.; Goto, Y.; Cheng, Y.; Gonda, H.; Inoue, N.; Nakagawa, S.; Manabe, N., 2008: Molecular cloning of a porcine (Sus scrofa) apoptosis inhibitory ligand, netrin-1, and its receptor, p53RDL1. The apoptosis inhibitory ligand (Netrin-1) and its receptor (p53-regulated receptor for death and life: p53RDL1) play an important role in the regulation of selective apoptosis. When Netrin-1 binds to p53RDL1, p53-dependent apoptosis is inhibited....

Farr K.L.; Montano C.Y.; Paxton L.L.; Savage D.D., 1988: Prenatal ethanol exposure decreases hippocampal tritiated glutamate binding in 45 day old rats. The effect of prenatal ethanol exposure on putative glutamate receptor binding sites in rat brain was studied using radiohistochemical techniques. Pregnant Sprague-Dawley rats were fed a liquid diet containing either 3% or 6% (vol./vol.) ethanol t...

Itoh, A.; Hall, P.; Maiello, L.; Di-Mario, C.; Moussa, I.; Blengino, S.; Ferraro, M.; Martini, G.; Di-Francesco, L.; Finci, L.; Colombo, A., 1997: Intracoronary stent implantation in native coronary arteries and saphenous vein grafts: A consecutive experience with six types of stents without prolonged anticoagulation. Objective: To analyze the results of implantation of six different intracoronary stents without the use of prolonged anticoagulation. Material and Methods: Between Mar. 30, 1993, and June 30, 1995, 889 patients with 1,194 coronary or vein graft le...

Wiedemann K., 1985: Human beta casein contains fragments with opioid activity. Naunyn Schmiedeberg'S Archives Of Pharmacologyuppl: R85