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Chicken embryo as an experimental model for the study of gastroschisis

, : Chicken embryo as an experimental model for the study of gastroschisis. Acta Cirurgica Brasileira 23(3): 247-252

To reproduce the experimental model of gastroschisis in chicken embryos and to prove that the histopathological changes that occur in this model can be compared to those in human gastroschisis. A total of 278 Leghorn hen (Gallus domesticus) eggs were used. The embryos were divided into three groups: the gastroschisis group, in which the umbilical cord was opened through an orifice made in the eggshell, and the intestinal loops were exposed to a mixture of amniotic liquid and allantoid; the mixture group, in which the amniotic fluid and allantoid were simply mixed without manipulating the umbilical stump and without exposing intestinal loops; and the control group which consisted of normal embryos in which no procedure was performed. The procedures were performed on the 13th day of embryo development and the study ended on the 19th day, when the intestinal loops of the embryos were removed and sent for conventional histological study and digital morphometric analysis. At the end of the experiment, 23 live embryos were obtained in the gastroschisis group (11.1% survival), and 18 of these presented exposed intestinal loops (8.7% success). The embryos of the gastroschisis group weighed less than those of the other two groups. The gastroschisis group also developed intestinal changes consisting of the thickening of the intestinal wall, inflammatory infiltration of the serosa and mucosa, ischemic changes in the intestinal wall and formation of a fibrin layer over the loops. These findings are characteristic of human gastroschisis and were not observed in the two other groups studied. The experimental model in chicken embryos proved able to reproduce the intestinal changes of human gastroschisis.


PMID: 18552995

Other references

Shade, R.O.; Blundell, M.C.; Cotmore, S.F.; Tattersall, P.; Astell, C.R., 1986: Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. The nucleotide sequence of an almost-full-length clone of human parvovirus B19 was determined. Whereas the extreme left and right ends of this genomic clone are incomplete, the sequence clearly indicates that the two ends of viral DNA are related...

Garcia-Vidal, C.; Fernández-Sabé, N.; Carratalà, J.; Díaz, V.; Verdaguer, R.; Dorca, J.; Manresa, F.; Gudiol, F., 2008: Early mortality in patients with community-acquired pneumonia: causes and risk factors. The first 48 h of evolution of patients with community-acquired pneumonia (CAP) are critical. The aim of the present study was to determine the frequency, causes and factors associated with early mortality in CAP. Nonimmunocompromised adults hospi...

Arnason T., 1982: Prediction of breeding values for multiple traits in small nonrandom mating horse populations. A mixed model method (BLUP) [best linear unbiased prediction] is introduced for the estimation of breeding values for multiple traits of all animals included in a dataset, by utilizing the complete genetic relationship. Considerable computational...

Murphy, C.; Cain, W.S.; Gilmore, M.M.; Skinner, R.B., 1991: Sensory and semantic factors in recognition memory for odors and graphic stimuli: elderly versus young persons. In four experiments, young (18-26 years, M = 21) and elderly (over 65 years, M = 72) people were compared for recognition memory of (a) graphic stimuli (faces of presidents and vice presidents, engineering symbols, and free forms) and (b) everyday...

Hickler, F.D., 1969: Symposium on design and function of the operating suite and special areas. Introduction--architectural planning and hospital ecology. Anesthesiology 31(2): 103-106

Vela Navarrete, R., 1988: Whatever became of trigonitis. Actas Urologicas Espanolas 12(3): 202-203

Silversides, Cr, 1981: How engineering science raised woods efficiency. Pulp and paper Canada 82(2): 33

Schlesinger, E.B.; Hazen, R., 1954: The cardiovascular effects of arteriovenous fistulae above and below the heart. Transactions of the American Neurological Association 13(79th Meeting): 214-216

Norman, Peter, 2004: Efficient Mechanisms for Public Goods with Use Exclusions. Constrained efficient provision of an excludable public good is studied in a model where preferences are private information. The provision level is asymptotically deterministic, making it possible to approximate the optimal mechanism with a mecha...

Pittaluga, S.; Loke, S.L.; So, K.C.; Cheung, K.N.; Ma, L., 1992: Clonal Epstein-Barr virus in lymphoepithelioma-like carcinoma of the stomach: demonstration of viral genome by in situ hybridization and Southern blot analysis. Lymphoepithelioma (LE), originally described in the nasopharynx, is an undifferentiated carcinoma with heavy lymphocytic infiltrate. The tumor is common in Southeast Asia, particularly in southern China, where the Epstein-Barr virus (EBV) associat...