+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chicken embryo cardiomyocyte cultures--a new approach for studying effects of halogenated aromatic hydrocarbons in the avian heart

, : Chicken embryo cardiomyocyte cultures--a new approach for studying effects of halogenated aromatic hydrocarbons in the avian heart. Toxicological Sciences 109(1): 66-74

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), polychlorinated biphenyls (PCBs), and other halogenated aromatic hydrocarbons (HAHs) elicit a variety of adverse biological effects on the cardiovascular systems of mammalian, piscine and avian species. Many of the cardiotoxic effects of HAHs are mediated by the aryl hydrocarbon receptor (AHR). Induction of cytochrome P4501A (CYP1A) is a well-known AHR-dependent response to HAHs in the liver, but there are a limited number of studies on CYP1A induction by these compounds in the heart. We used an in vitro approach to examine effects of TCDD and 3,3',4,4'-tetrachlorobiphenyl (PCB 77) on CYP1A in the avian heart. The responses of primary cultures of chicken embryo cardiomyocytes (CEC) and chicken embryo hepatocytes (CEH) to TCDD and PCB 77 were compared using immunofluorescence staining for CYP1A, the ethoxyresorufin-O-deethylase (EROD) assay, and real-time RT-PCR analysis of CYP1A4 mRNA and CYP1A5 mRNA. Immunofluorescent detection of CYP1A indicated that induction of CYP1A by TCDD was localized within the cytoplasm of CEC cells. EROD activity and CYP1A4/5 mRNA levels were strongly induced in CEC and CEH cultures by TCDD and PCB 77, and the shapes of the concentration-response curves in CEC and CEH cultures were similar. The studies provide clear evidence that the AHR signaling pathway is induced by TCDD and PCB 77 in CEC, and establish a new in vitro approach for studying the effects of HAHs in the avian heart. Induction of CYP1A5 by TCDD in avian cardiomyocytes is a novel finding, and might help direct future studies on mechanisms of action of HAHs in the heart.


PMID: 19223662

DOI: 10.1093/toxsci/kfp039

Other references

Siddiquie H.N.; Rao P.S., 1988: Exploration and polymetallic nodules in the Indian Ocean. Ocean Development and International Law 19(4): 323-335

Brown N.K.; Harvey D.J., 1987: Metabolism and potency of tetrahydrocannabinol homologues. British Journal of Pharmacology 92(SUPPL): 724P

Fluckiger, E.; Walser, F., 1978: Analysis of fermentation of maturing Emmental cheese with special consideration of dissolved CO2. Analysis of Emmental cheese for changes occurring during the 16 wk following preparation was carried out on 16 approx. 3-kg cubical blocks symmetrically carved from a single cheese loaf. Production of CO2 was found to precede the onset of propioni...

Udaka, H., 1977: Clinical and statistical studies on congenital dilatation of the biliary tract in relation to anomalous arrangement of the pancreatico biliary ducts. Six cases of anomalous arrangement of the pancreatiocobiliary ducts were found in 16 cases of congenital dilatation of the biliary tract in the past 5 yr. This high incidence of the anomaly prompted an investigation of the incidence in Japan. Case...

Grinenko L.N., 2000: Two levels of crustal contamination for continental basalt magma. Abstract Volume (Geological Association of Canada) 25: (unpaginated

Aral, S.O.; Fenton, K.A.; Holmes, K.K., 2007: Sexually transmitted diseases in the USA: temporal trends. This paper reviews the temporal trends in sexually transmitted diseases (STDs) and discusses the factors affecting the epidemiology of bacterial STDs.

Mantel N., 1971: On greens test for dependence. Biometrics 27(2): 444-447

Laxminarayana, K.; Patiram, 2005: Influence of inorganic, biological and organic manures on yield and nutrient uptake of groundnut (Arachis hypogaea) and soil properties. A field experiment was conducted in Mizoram, India, in Ultisols during the 2001 and 2002 rainy seasons, to study the effect of the integrated use of inorganic, biological and organic manures on yield and nutrient uptake of groundnut and their effe...

Kaplan, M., H.; Sadick, N., S.; Mcnutt,; Talmor, M.; Coronesi, M.; Hall, W.W., 1993: Acquired ichthyosis in concomitant HIV-1 and HTLV-II infection: A new association with intravenous drug abuse. Background: Papulosquamous eruptions are common in HIV-1-infected patients. Acquired ichthyosis may occur after profound T-cell depletion. Intravenous drug users infected with HIV-1 can be coinfected with human lymphotropic virus II (HTLV-11). Obj...

Saradzhishvili P.M.; Gabashvili V.M., 1975: Clinical picture and pathogenesis of nocturnal cerebral strokes. Zhurnal Nevropatologii i Psikhiatrii Imeni S S Korsakova 75(7): 975-980