+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chondrogenic differentiation of ChM-I gene transfected rat bone marrow-derived mesenchymal stem cells on 3-dimensional poly (L-lactic acid) scaffold for cartilage engineering

, : Chondrogenic differentiation of ChM-I gene transfected rat bone marrow-derived mesenchymal stem cells on 3-dimensional poly (L-lactic acid) scaffold for cartilage engineering. Cell Biology International 39(3): 300-309

We have explored the role of Chondromodulin-I (ChM-I) in chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) in 3-dimensional (3D) scaffold for cartilage tissue engineering. BMSCs of Sprague Dawley (SD) rats were cultured on poly-(L-lactic acid) [PLLA] scaffolds with different pore sizes (80-200 μm, 200-450 μm) with or without surface modification by chitosan. Cell viability, proliferation, and morphology were measured using confocal microscope and the CCK-8 method. Untransfected BMSCs, BMSCs expressing pcDNA3.1(+), BMSCs expressing plasmid pcDNA3.1 (+)/ChM-I were cultured on 3D scaffolds in standard growth medium or transforming growth factor-β1 (TGF-β1) supplemented chondrogenic induction medium in vitro for 3 weeks and the expression of collagen type II was determined. Cell-scaffolds constructs were implanted subcutaneously for 3 months in vivo. BMSCs had a higher viability and proliferation in PLLA scaffolds of pore size 200-450 μm than that of 80-200 μm, and surface modification with chitosan did not enhance cell attachment. The ChM-I gene enhanced chondrogenesis and increased collagen type II synthesis. Immunohistochemistry from in vivo study showed enhanced cartilage regeneration in BMSCs expressing pcDNA3.1 (+)/ChM-I on 3D PLLA scaffolds. It also demonstrated that TGF-β1 might promote chondrogenesis of rat BMSCs by synergizing with the ChM-I gene. ChM-I could be beneficial to future applications in cartilage repair.


PMID: 25319137

DOI: 10.1002/cbin.10393

Other references

Heymann, D.L.; Belsey, E.; Esparza, J.G., 1993: CD4+ T-lymphocytopenia without HIV infection. New England Journal of Medicine 328(25): 1848; Author Reply 1849-50

Seecof, R.L., 1966: Sigma virus content and hereditary transmission in Drosophila melanogaster. Three strains of Drosophila infected with sigma virus were selected for 20 generations for flies producing high proportions of virus-infected eggs. The following strain differences were detected before selection and shown to persist after the sele...

Storch, V.; Welsch, U., 1970: Der Bau der Korperwand von Leucochloridium paradoxum. The fine structure of the body-wall of the sporocyst and tailless cercaria of Leucochloridium paradoxum was examined by electron microscopy. The integument of the sporocyst is characterized by long branched microvilli and by perikarya, lying below...

Coulange, C.; Leremboure, H.; Hernandez, F.; Albert, P.; Rampal, M., 1987: Cancer of the kidney and hepatic tumor: a therapeutic problem. Journal D'urologie 93(9-10): 575-576

Spellman, C.M.; Fottrell, P.F., 1972: Inhibition of human lactate dehydrogenase isoenzymes by oestradiol-17-beta in vitro. Febs Letters 21(2): 186-188

Jiménez, M.; Pérez-Urizar, J.; Flores-Murrieta, F.J., 2000: Influence of sex on the pharmacokinetics of tolmetin in the rat. The purpose of this study was to investigate sex-related differences in the pharmacokinetics of tolmetin, a potent nonsteroidal anti-inflammatory drug, in the rat. Male and female Wistar rats received oral tolmetin at two dose levels, 3.2 and 10 m...

Hettelingh, J.P.; Gardner, R.H.; Hordijk, L., 1992: A statistical approach to the regional use of critical loads. The premise of this paper is that: (1) effects of spatial heterogeneity of watershed response to acid deposition must be considered when models are used to set abatement policies, and (2) the evaluation of critical chemical values is a better meas...

Rudman, W.B., 1971: The family Acteonidae (Opisthobranchia,Gastropoda) in New Zealand. Journal of the Malacological Society of Australia, 2: 205-214

Bould, C., 1964: Manurial experiments with fruit: VI. A factorial NPK experiment with black currants, var. Baldwin and Mendip Cross. The final results confirmed the earlier findings that fertilizer N at rates above the equivalent of 3 cwt./acre of ammonium sulfate had either no significant effect on, or depressed, growth and yield, but that fertilizer P and K increased yield. F...

Cardinal Damien; Fagel Nathalie; Andre Luc; Dehairs Frank, 1999: Ba repartition in surface sediment and trap material; a sequential leaching approach. Journal of Conference Abstracts: Pages 245. 1999.