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Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae


, : Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 65(Pt 9): 952-955

Xanthomonas oryzae pv. oryzae (Xoo) causes the serious disease bacterial blight in rice. The pepA (Xoo0834) gene from Xoo is one of around 100 genes that have been selected for the design of antibacterial drugs. The pepA gene encodes leucine aminopeptidase (LAP), an exopeptidase that catalyzes the hydrolysis of leucine residues from the N-terminus of a protein or peptide. This enzyme was expressed in Escherichia coli, purified and crystallized, and preliminary X-ray structural studies have been carried out. The LAP crystal diffracted to 2.6 A resolution and belonged to the cubic space group P2(1)3. The unit-cell volume of the crystal was compatible with the presence of two monomers in the asymmetric unit.

US$19.90

PMID: 19724142

DOI: 10.1107/S1744309109031467


Other references

Pincus, T.; Hartley, J.W.; Rowe, W.P., 1975: A major genetic locus affecting resistance to infection with murine leukemia viruses. IV. Dose-response relationships in Fv-1-sensitive and resistant cell cultures. Virology 65(2): 333-342

Breuer H W.M.; Skyschally A.; Schulz R.; Heusch G., 1992: Poor reproducibility of heart rate variability indices in the frequency domain and in the time domain. FASEB Journal 6(4): A1160

Mitsushima T.; Arima N.; Nagatani K.; Kokubo T.; E.A., 1989: Distribution of neoplastic polyp in japanese adults estimation based on five years experience of colonoscopic mass screening. In order to know the natural distribution of neoplastic polyp of the large bowel in Japanese adults, we analyzed the data obtained from the patients who had received colonoscopy for cancer screening. Out of 4,959 patients, 16 early carcinomas (0.3...

Tagowski, M.; Kamusella, P.; Andresen, R., 2014: A rare cause of abdominal pain: emphysematous cystitis. Rofo 185(10): 998-999

Gonzalez, Y.; Iwasaki, L.R.; McCall, W.D.; Ohrbach, R.; Lozier, E.; Nickel, J.C., 2011: Reliability of electromyographic activity vs. bite-force from human masticatory muscles. The reproducibility of electromyographic (EMG) activity in relation to static bite-force from masticatory muscles for a given biting situation is largely unknown. Our aim was to evaluate the reliability of EMG activity in relation to static bite-f...

Martel, M.-Kaye; Eisbruch, A.; Lawrence, T., S.; Fraass, B., A.; Ten-Haken, R., K.; Lichter, A., S., 1998: Spinal cord dose from standard head and neck irradiation: Implications for three-dimensional treatment planning. Background and purpose: Treatment with traditional standard field arrangements for patients with head and neck cancer rarely causes myelopathy. Often, initial treatment fields are reduced to avoid the spinal cord after 45 Gy has been delivered and...

Baud, V.; Lipinski, M.; Rassart, E.; Poliquin, L.; Bergeron, D., 1991: The human homolog of the mouse common viral integration region, FLI1, maps to 11q23-q24. FLI1 is a common mouse viral integration region in virus-induced leukemias and lymphomas. Using an evolutionarily conserved mouse probe and Southern hybridization to (rodent x human) somatic cell hybrid DNAs, the human homolog of FLI1 has been sho...

Besri, M., 1978: Phases of the transmission of Fusarium oxysporum f. sp. lycopersici and Verticillium dahliae by seeds of some tomato varieties. F. oxysporum f.sp. lycopersici was found in all parts of the fruit clusters (peduncle, fruit vascular tissue, seed) of infected plants of the 3 most important autumn cvs. grown in Morocco [RPP 57, 2652]. The lower fruit clusters were more infected...

Herkner, H.; Eisenburger, P.; Havel, C.; Laggner, A.N., 2002: Dashing with scooters to in-hospital emergencies: a randomised cross-over experiment. Physical exhaustion is a frequent condition in emergency medical teams after in-house emergency runs, which might affect the quality of advanced care. Newly available light-weight scooters may reduce exertion as measured by the cardiovascular resp...

Strizhevskii I.I., 1982: Safety and protection of the environment during production of ammonium nitrate and fertilizer based on it. Thermal decomposition of ammonium nitrate and its explosive properties were discussed. Production of ammonium nitrate and purification of steam and gas waste were considered. Fertilizers containing ammonium nitrate were classified into 4 groups wi...