geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice


, : Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice. Haematologica 99(3): 588-596

The offspring from pregnancies of women who have developed anti-D blood group antibodies are at risk of hemolytic disease of the newborn. We have previously mapped four peptides containing immunodominant T-helper cell epitopes from the RhD protein and the purpose of the work was to develop these into a product for suppression of established anti-D responses. A panel of each of the four immunodominant RhD peptides was synthesized with modifications to improve manufacturability and solubility, and screened for retention of recognition by human T-helper cells. A selected version of each sequence was combined in a mixture (RhDPmix), which was tested for suppressive ability in a humanized murine model of established immune responses to RhD protein. After HLA-DR15 transgenic mice had been immunized with RhD protein, a single dose of RhDPmix, given either intranasally (P=0.008, Mann-Whitney rank sum test) or subcutaneously (P=0.043), rapidly and significantly suppressed the ongoing antibody response. This was accompanied by reduced T-helper cell responsiveness, although this change was less marked for subcutaneous RhDPmix delivery, and by the recruitment of cells with a regulatory T-cell phenotype. The results support human trials of RhDPmix peptide immunotherapy in women with established antibody responses to the RhD blood group.

US$19.90

PMID: 24441145

DOI: 10.3324/haematol.2012.082081


Other references

Lv, C.; Zenisek, D., 2015: Big minis from hair cells: mechanism and function. What underlies the large variation in mEPSC amplitude in the auditory system? And is this variability important? In this issue of Neuron, Li et al. (2014) address the significance of large mEPSCs to auditory processing and Chapochnikov et al. (201...

Andrews, G.L.; Tanglao, S.; Farmer, W.Todd.; Morin, S.; Brotman, S.; Berberoglu, M.A.; Price, H.; Fernandez, G.C.; Mastick, G.S.; Charron, Fédéric.; Kidd, T., 2008: Dscam guides embryonic axons by Netrin-dependent and -independent functions. Developing axons are attracted to the CNS midline by Netrin proteins and other as yet unidentified signals. Netrin signals are transduced in part by Frazzled (Fra)/DCC receptors. Genetic analysis in Drosophila indicates that additional unidentifie...

Echols, P.G.; Omer, G.E.; Crawford, M.K., 2000: Juxta-articular myxoma of the shoulder presenting as a cyst of the acromioclavicular joint: a case report. Journal of Shoulder and Elbow Surgery 9(2): 157-159

Plough, H.H.; Roberts, M., 1955: High frequency of transduction of genes by bacteriophage in Salmonella. A frequency of one H+ transduction for every 1000 of the free phage particles isolated from a lysogenic strain of S. typhimurium when undergoing occasional lysis is reported. This frequency is almost a thousand times greater than that previously f...

Stephanova, D.I.; Daskalova, M., 2004: Excitability properties of normal and demyelinated human motor nerve axons. The strength-duration time constants and rheobase currents, which provide an indirect indication of the axonal properties are calculated in two cases of stimulation, using our previous double cable models of normal and demyelinated human motor fib...

Marcellin, P., 1974: Controlled atmosphere storage of vegetables in polyethylene bags with silicon rubber window. Acta Horticulturae 38 (1): 33-45

Bender, R.A.; Dube, C.; Baram, T.Z., 2000: Mossy fiber sprouting into the inner molecular layer of the dentate gyrus follows prolonged febrile seizures in the immature rat model. Epilepsia 41(Supplement 7): 76

Zhao, Y.; Wang, Q.; Meng, F.; Lin, Y.; Wang, S.; Li, Y.; Lin, B.; Cao, S.; Cao, J.; Fang, Z.; Li, T.; Zang, E., 2012: High-finesse cavity external optical feedback DFB laser with hertz relative linewidth. We report hertz level relative linewidth distributed feedback diode lasers with external optical feedback from a high finesse F-P cavity, and demonstrate the efficient phase noise suppression and laser linewidth reduction of the optical feedback t...

Sheppard, D.; Chen, A.; Weinacker, A.; Agrez, M., 1994: Distinct regions of the cytoplasmic domain of the integrin beta-6 subunit are required for localization to focal contacts and for stimulation of epithelial cell proliferation. Journal of Cellular Biochemistry Supplement 0(18C): 256

Sberegaeva, A.V.; Zavalij, P.Y.; Vedernikov, A.N., 2016: Oxidation of a Monomethylpalladium(II) Complex with O2 in Water: Tuning Reaction Selectivity to Form Ethane, Methanol, or Methylhydroperoxide. Photochemical aerobic oxidation of n-Pr4N[(dpms)Pd(II)Me(OH)] (5) and (dpms)Pd(II)Me(OH2) (8) (dpms = di(2-pyridyl)methanesulfonate) in water in the pH range of 6-14 at 21 °C was studied and found to produce, in combined high yield, a mixture of...