geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Comparative study of one-step nucleic acid amplification assay, frozen section, and touch imprint cytology for intraoperative assessment of breast sentinel lymph node in Chinese patients


, : Comparative study of one-step nucleic acid amplification assay, frozen section, and touch imprint cytology for intraoperative assessment of breast sentinel lymph node in Chinese patients. Cancer Science 103(11): 1989-1993

Conventional procedures for the intraoperative assessment of breast cancer sentinel lymph nodes (SLNs) are frozen section (FS) and touch imprint cytology (TIC). The one-step nucleic acid amplification (OSNA) assay is a novel molecular technique. The aim of this study was to evaluate the optimal approach by comparing OSNA assay, FS, and TIC. Five hundred and fifty-two consecutive patients were enroled from five study centers in China. The SLNs were cut into alternating 2 mm blocks. The odd blocks were tested by the OSNA assay intraoperatively, and the even ones were assessed by postoperative histology (four 4- to 6-μm-thick sections were taken every 200 μm per block). In addition, intraoperative histological assessments were carried out on the even blocks of 211 patients by FS and all blocks of 552 patients by TIC. Overall performance of the assay compared to postoperative histology was: accuracy 91.4%; sensitivity 83.7%; and specificity 92.9%. The sensitivity of the assay was higher than FS (211 patients, 77.6% vs 69.7%; not significant, P = 0.286) and was significantly higher than TIC (552 patients, 83.6% vs 76.2%; P = 0.044). When assessing nodes with micrometastases, the sensitivity of the assay was higher than FS (17 nodes, 47.1% vs 23.5%; not significant, P = 0.289) and was significantly higher than TIC (48 nodes, 62.5% vs 35.4%; P = 0.007). The study indicated that the OSNA assay is an accurate and rapid intraoperative assay for assessing breast SLNs and it can replace FS and TIC for application in general medical practice. The trial was registered as: OSNA assay China Registration Study. China Breast Cancer Clinical Study Group 001c.

US$19.90

PMID: 22924886

DOI: 10.1111/cas.12001


Other references

Yamazaki, S., 1982: Roles of the referee system in scientific journals. Kango Tenbo. Japanese Journal of Nursing Science 7(9): 784-788

Ojehomon, O.O.; Morgan, D.G., 1969: A quantitative study of inflorescence development in Phaseolus vulgaris. The sequence and pattern of inflorescence differentiation in 6 determinate and 3 indeterminate varieties of Phaseolus vulgaris. -Cambridge Univ.

Omrane, S.; Ferrarini, A.; D'Apuzzo, E.; Rogato, A.; Delledonne, M.; Chiurazzi, M., 2009: Symbiotic competence in Lotus japonicus is affected by plant nitrogen status: transcriptomic identification of genes affected by a new signalling pathway. In leguminous plants, symbiotic nitrogen (N) fixation performances and N environmental conditions are linked because nodule initiation, development and functioning are greatly influenced by the amount of available N sources. We demonstrate here th...

Mela, P.; Veltink, P.H.; Huijing, P.A., 2002: Excessive reflexes in spinal cord injury triggered by electrical stimulation. Interaction of electrocutaneous stimulation with an impaired human motor control system may result in unstable reflex loops causing excessive spastic reactions. These contractions are usually excluded from analysis since the presence of spasm is o...

Fried, I., 2000: Functional neuroimaging in presurgical localization of essential cortical processing zones. Advances in Neurology 83: 297-303

Zacharias Langhans, Andres, 1997: Studies on copper and zinc contamination of leaf litter of roadside trees in Hamburg. The amount of copper and zinc in the leaf litter of different tree species in Hamburg was analyzed in relation to traffic density and other possible factors of contamination, such as copper roofs and railway bridges. The leaf litter was digested w...

Kato R.; Onuki A.S.; Watanabe M.; Hashizume T.; Kawamura M.; Kikuchi K.; Kobayashi K.; Ishihara T., 1990: Tracheal reconstruction by esophageal interposition an experimental study. The purpose of this study was to assess the possibility of recontructing a circumferential tracheal defect with autogenous esophagus. In 6 mongrel dogs, a circumferential defect involving seven rings of the cervical trachea was reconstructed by in...

Allison, N.; Millward, G., E.; Jones, M., B., 1998: Particle processing by Mytilus edulis: Effects on bioavailability of metals. Mytilus edulis were maintained in laboratory flow-through systems and fed estuarine permanently suspended particulate matter (PSPM), of particle diameters from < 3 to 40 mum. The concentrations of Fe, Mn, Zn, and Cu in the PSPM, and in the faec...

Jaksch, Walter, 1961: Observations and experiments in the use of oxytetracycline for treatment of internal diseases in horses, carnivores, and poultry. The efficiency of oxytetracycline was tested in 29 horses, 674 dogs, 6 cats and about 3800 chickens for contagious bronchitis, petechial fever, aspiration pneumonia, malignant edema, gastro-enteritis and acute eczemal, in the horse; distemper, lep...

Wells, A.U.; Hansell, D.M.; Rubens, M.B.; Cullinan, P.; Haslam, P.L.; Lawrence, R.; D.B.is, R.M.; Black, C.M., 1992: Indices of lung inflammation predict disease severity in scleroderma lung disease as assessed by HRCT. Arthritis & Rheumatism 35(9 SUPPL ): S205