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Comparative transcriptome analysis of human aorta atherosclerotic lesions and peripheral blood leukocytes from essential hypertension patients

, : Comparative transcriptome analysis of human aorta atherosclerotic lesions and peripheral blood leukocytes from essential hypertension patients. Kardiologiia 49(9): 27-38

One of the major cardiovascular risk factor which predisposes to and accelerates atherosclerosis is arterial hypertension (AH). To determine the molecular basis of the crosslink between AH and atherosclerosis for the development of new treatment strategies large-scale transcriptome analysis of the cells implicated in atherogenesis is needed. We used cDNA microarray technique for simultaneous analysis of gene expression in human abdominal aorta normal sites and atherosclerotic lesions of different histological types, as well as in peripheral blood leukocytes from patients with essential hypertension (EH) and donors. The microarray data were verified by quantitative RT-PCR (reverse transcription coupled with polymerase chain reaction) and immunohistochemical analysis. Differential expression of 40 genes has been found, among which twenty two genes demonstrated up-regulation and 18 genes demonstrated down-regulation in atherosclerotic aorta compared with normal vessel. New gene-candidates, implicated in atherogenesis, have been identified - FPRL2, CD37, CD53, RGS1, LCP1, SPI1, CTSA, EPAS1, FHL1, GEM, RHOB, SPARCL1, ITGA8, PLN, and COL14A1. These genes participate in cell migration and adhesion, phenotypic changes of smooth muscle cells, immune and inflammatory reactions, oxidative processes and extracellular matrix remodeling. We have found increased expression levels of CD53, SPI1, FPRL2, SPP1, CTSD, ACP5, LCP1, CTSA and LIPA genes in peripheral blood leukocytes from EH patients and in atherosclerotic lesions of human aorta. The majority of these genes significantly (p<0.005) positively (r>0.5) correlated with AH stage as well as with histological grading of atherosclerotic lesions.


PMID: 19772500

Other references

Exner, T.; Richard, K.A., 1974: An improved procedure for determining thromboplastin generation. Journal of Clinical Pathology 27(8): 683-684

Janosi, J.; Sebestyen, A.; Mikala, G.; Peto, M.; Jako, J.; Domjan, G.; Nemeth, J.; Kiss, Z.; Kopper, L.; Valyi Nagy, I., 2003: Soluble syndecan-1 is a prognostic marker in multiple myeloma. Syndecans are a family of cell surface proteoglycans. In the bone marrow of multiple myeloma patients syndecan-1 is expressed only on the surface of malignant plasma cells. In this study we analyzed the serum level of shed syndecan-1 in three grou...

Bills, C.E., 1938: The chemistry of Vitamin D. A very lucid account is given of the 10 known sterol derivatives which show vitamin D activity. Of these the chemistry of 5 is explained, and of the other 5 the fragmentary existing knowledge is summarised. E. M. Hume.

Kecskés-Kovács, K.; Sulykos, Ián.; Czigler, Ián., 2013: Visual mismatch negativity is sensitive to symmetry as a perceptual category. We investigated the sensitivity of visual mismatch negativity (vMMN) to an abstract and non-semantic category, vertical mirror symmetry. Event-related potentials (ERPs) elicited by random and symmetric square patterns, delivered in passive oddball...

Persing, C. 0., 1935: A discussion of various oils in spray combinations with lead arsenate, cryolite and barium flue-silicate. Straight mineral oil and mineral oil have been used extensively in the past with lead arsenate and fluorine compounds, with variable results. Exps. indicated that the wetting property of an oil is probably the most important single factor that det...

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Truin, G.J.; Frencken, J.E.; Mulder, J.; Kootwijk, A.J.; Jong,, 2007: Prevalence of caries and dental erosion among school children in The Hague from 1996-2005. In 2005 a dental survey of the prevalence of caries among 6- and 12-year-old schoolchildren in The Hague was carried out. In the case of the 12-year-olds, the prevalence of dental erosion was also studied. The sample consisted of 814 students in t...

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Chiodetti, L.M.; Barber, D.L.; Schwartz, R.H., 2000: Biallelic expression of the interleukin-2 locus under optimal stimulation conditions. FASEB Journal 14(6): A1167, April 20

Koren R.; Loeb L.A.; Mildvan A.S., 1976: Epr and kinetic studies of the role of the divalent cation in rna polymerase from escherichia coli. Federation Proceedings 35(7): 293