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Comparison of five methods for the determination of rubella immunity


, : Comparison of five methods for the determination of rubella immunity. Infectious Diseases in Obstetrics and Gynecology 1(4): 188-192

The purpose of this study was to compare the accuracy of commonly used methods for the detection of rubella immunity, especially the fully automated IMx assay. A total of 190 sera (101 immune and 89 non-immune) submitted to Harrisburg Hospital or Polyclinic Medical Center for the determination of rubella immunity were tested by enzyme immunoassay (IMx and Rubazyme, Abbott Diagnostic Laboratories, North Chicago, IL), indirect immunofluorescence (FIAX, Whittaker Bioproducts, Walkersville, MD), and latex agglutination (Rubascan, Becton Dickinson Microbiology Systems, Cockeysville, MD, and Rubalex, Wellcome Diagnostics, Research Triangle Park, NC). Specimens were frozen at -30 until the study was initiated. Each of the assays was performed according to the manufacturers' specifications. Sensitivity, specificity, accuracy, and positive and negative predictive values for each assay were calculated using a consensus result of the 5 methods tested. The sensitivity, specificity, and accuracy, respectively, of the test systems were as follows: IMx, 96%, 97%, and 96%; Rubazyme, 100%, 99%, and 99%; Rubascan, 100%, 98%, and 99%; Rubalex, 99%, 97%, and 98%; and FIAX 90%, 100%, and 95%. False negative reactions were seen with the FIAX system. The IMx system, a new "walk away" system from Abbott Diagnostic Laboratories and the Rubazyme systems performed well; however the latex agglutination tests proved to be the most rapid and convenient methods for screening sera for the presence of rubella immunity.

US$19.90

PMID: 18475343

DOI: 10.1155/S1064744994000062


Other references

Millet, J-Pablo.; Orcau, A.; Casals, Mí.; Garcia de Olalla, P.; Caylà, J.A., 2014: Recurrences in tuberculosis in a cohort of human immunodeficiency virus-infected patients: the influence of highly active antiretroviral therapy. Little is known about recurrent tuberculosis (TB) among HIV-infected patients and the influence of highly active antiretroviral therapy (HAART). A population-based retrospective longitudinal study was conducted on all HIV-infected TB patients in B...

Cullen, L.; Adams, S.L., 2012: Planning for implementation of evidence-based practice. Expectations for evidence-based healthcare are growing, yet the most difficult step in the process, implementation, is often left to busy nursing leaders who may be unprepared for the challenge. Selecting from the long list of implementation strat...

Wei, S.; Fielding, E.; Leprince, S.; Sladen, A.; Avouac, J.-Philippe; Helmberger, D.; Hauksson, E.; Chu, R.; Simons, M.; Hudnut, K.; Herring, T.; Briggs,; Richard, 2011: Superficial simplicity of the 2010 El Mayor-Cucapah earthquake of Baja California in Mexico. Nature Geoscience 4.9

Heskova, A., 1978: Influence of food change on the pathology of caterpillars of Lymantria dispar L. Acta Instituti forestalis Zvolenensis(5): 159-176

Shiroza S., 1985: Production and characterization of anti insulin monoclonal antibody. Diabetes Research & Clinical Practice. 1: S262

Jung, Y-Sang.; Chun, H-Young.; Yoon, M-Ho.; Park, B-Joon., 2015: Elevated estrogen receptor-α in VHL-deficient condition induces microtubule organizing center amplification via disruption of BRCA1/Rad51 interaction. Since loss of VHL is frequently detected early phase genetic event in human renal cell carcinoma, pVHL is assumed to be indispensable for suppression of tumor initiation step. However, induction of HIF-1α, target of pVHL E3 ligase, is more adequa...

Schroth Martin H.; Field Jennifer A.; Humphrey Mark D.; Istok Jonathan D.; Sawyer Thomas E., 1998: Field method for the feasibility assessment of surfactant-enhanced DNAPL remediation. Pages 199-204 1998

Burkhart, K.Josef.; Nowak, T.E.; Kim, Y-Joo.; Rommens, P.M.; Müller, L.P., 2011: Anatomic fit of six different radial head plates: comparison of precontoured low-profile radial head plates. Bulky implants may lead to symptomatic soft tissue irritation after open reduction and internal fixation of radial head and neck fractures. The purpose of our study was to compare the anatomic fit of precontoured radial head plates. We stripped 22...

Morien, K., 1998: Benefits of artichoke leaf extract in hypercholesterolemia, dyspepsia, and liver function. HerbalGram (44) 21-22

Mendis, B.G.; Mackenzie, M.; Craven, A.J., 2009: A new analytical method for characterising the bonding environment at rough interfaces in high-k gate stacks using electron energy loss spectroscopy. Determining the bonding environment at a rough interface, using for example the near-edge fine structure in electron energy loss spectroscopy (EELS), is problematic since the measurement contains information from the interface and surrounding matr...