geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Constitutive activity of the lutropin receptor and its allosteric modulation by receptor heterodimerization


, : Constitutive activity of the lutropin receptor and its allosteric modulation by receptor heterodimerization. Methods in Enzymology 484: 231-252

The lutropin receptor (LHR) is a G protein-coupled receptor (GPCR) that mediates the actions of pituitary LH in males and females and that of placental hCG in pregnant women and, therefore, plays an essential role in reproductive physiology. Mutations of the lhcgr gene that result in constitutive activation of the LHR have been shown to be causative of gonadotropin-independent precocious puberty in young boys. Studies on constitutively active mutants (CAMs) of the LHR have been extremely informative in elucidating the roles of the LHR in reproductive physiology as well as in understanding the molecular basis underlying activation of this GPCR. The constitutive activities of hLHR CAMs can be attenuated by introducing mutations into the CAMs that stabilize the resting state of the hLHR or by coexpressing the hLHR CAMs with an hLHR mutant that is stabilized in the resting state, allowing the two forms of the hLHR to heterodimerize. This chapter describes the experimental methods and strategies underlying studies of hLHR CAMs.

US$19.90

PMID: 21036235

DOI: 10.1016/B978-0-12-381298-8.00012-5


Other references

Knowlton, N.P.; Widner, W.R., 1950: The use of X-rays to determine the mitotic and intermitotic time of various mouse tissues. Cancer Research 10(1): 59-63

Florio, P.; Imperatore, A.; Sanseverino, F.; Torricelli, M.; Reis, F.M.; Lowry, P.J.; Petraglia, F., 2004: The measurement of maternal plasma corticotropin-releasing factor (CRF) and CRF-binding protein improves the early prediction of preeclampsia. In the present study we measured maternal plasma concentrations of two placental neurohormones, corticotropin-releasing factor (CRF) and CRF-binding protein (CRF-BP), in 58 at-risk pregnant women consecutively enrolled between 28 and 29 wk of preg...

Poonja, L.S.; Bhatt, A.P.; Dholakia, H.M., 1979: Neurilemoma of the oral cavity. Journal of the Indian Dental Association 51(3): 75-79

Husain K.; Vijayaraghavan R.; Marjit D.M., 1990: The effect of pyridostigmine and physostigmine on acute toxicity of dfp in rats. Diisopropyl fluorophosphate (DFP) given to rats in lethal concentration (100 mg/m3, by inhalation for 40 min) significantly inhibited acetylcholinesterase activity in the blood, lung, liver and brain, and induced hyperglycaemia and glycogen mobili...

Seitzman, B.A.; Abell, M.; Bartley, S.C.; Erickson, M.A.; Bolbecker, A.R.; Hetrick, W.P., 2016: Cognitive manipulation of brain electric microstates. NlmCategory="UNASSIGNED">EEG studies of wakeful rest have shown that there are brief periods in which global electrical brain activity on the scalp remains semi-stable (so-called microstates). Topographical analyses of this activity h...

Weitzman, C.; Nadler, C.B.; E Murray, K.; Moore, R.; Sia, J.H., 2016: Journal Article Reviews. Journal of Developmental and Behavioral Pediatrics 37(4): 346-351

Gronbaek, K.; Krarup, H.B.gum; Ring Larsen, H.; D.M.ckadell, O.S.haffalitzky; Moller, A.; Schlichting, P.; Vyberg, M., 2002: Interferon alfa-2b alone or combined with recombinant granulocyte-macrophage colony-stimulating factor as treatment of chronic hepatitis C. To compare the effect of combination therapy with interferon-alpha (INF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to monotherapy with INF-alpha in patients with chronic hepatitis C infection. Forty-five consecutive patie...

Beale, A., 2003: Gastrostomy insertion. Clinical Radiology 58(10): 821; Author Reply 821-821; Author Reply 821

Reis do Carmo, W.; Ferreira, F.Furlan.; Diniz, R., 2014: Phase quantification of antihypertensive drugs - Chlorthalidone, Hydrochlorothiazide, Losartan and combinations, Losartan/Chlorthalidone and Losartan/Hydrochlorothiazide - by the Rietveld method. The identification and quantification of crystalline phases of antihypertensive drugs - Losartan potassium (LOS-K), Hydrochlorothiazide (HCTZ) and Chlorthalidone (CTD) were carried out by means of X-ray powder diffraction data and the Rietveld met...

Govindaraj, P.; Nalini, A.; Krishna, N.; Sharath, A.; Khan, N.Akhtar.; Tamang, R.; Gourie-Devi, M.; Brown, R.H.; Thangaraj, K., 2014: Mitochondrial DNA variations in Madras motor neuron disease. Although the Madras Motor Neuron Disease (MMND) was found three decades ago, its genetic basis has not been elucidated, so far. The symptom at onset was impaired hearing, upper limb weakness and atrophy. Since some clinical features of MMND overla...