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Construction of directional differentiation model from mouse embryonic stem cells to Leydig-like cells in vitro

, : Construction of directional differentiation model from mouse embryonic stem cells to Leydig-like cells in vitro. Zhejiang Da Xue Xue Bao. Yi Xue Ban 41(4): 386-392

To construct a directional differentiation model from mouse embryonic stem cells into leydig-like cells in vitro. Mouse ES-D3 cells were transfected with plasmid containing steroidogenic factor 1 (SF-1) gene, then treated with RA and 8Br-cAMP, while the cells transfected with empty plasmid were used as the negative controls. The morphology of leydig-like cells differentiated from ES-D3 cells was observed with light microscopy. The expression levels of StAR, P450scc and 3β-HSD were detected by RT-PCR, Western Blot and fluorescence microscopy analysis in leydig-like cells derived from the ES cells. ES-D3 cells were transfected with plasmid containing SF-1 gene successfully, and SF-1 was expressed 24 h after transfection. The SF-1-transfected ES-D3 cells were induced by RA and 8Br-cAMP to differentiate into leydig-like cells. The differentiated cells showed spindle shape with tentacles, which expressed the specific protein marker for leydig cells 3β-HSD1 and P450scc. Meanwhile, in these leydig-like cells, the expression of StAR increased compared with control group. 3β-HSD1, P450scc and StAR were not detected in negative control group. When the ES-D3 cells are transfected with SF-1 plasmid and then treated with RA and 8Br-cAMP, the cells are able to differentiate into leydig-like cells, indicating that the model of directional differentiation of ES cells into leydig-like cells has been constructed successfully.


PMID: 22927073

Other references

Doult, B.; Stokes, B., 1997: Government orders review of record keeping by nurses. NlmCategory="UNASSIGNED">Stricter procedures for nursing records are being prepared by the government under the leadership of England's chief nursing officer, Yvonne Moores.

Nissen, G., 1986: Treatment for depression in children and adolescents. In the management of depressive states in children and adolescents a distinction should be drawn between: General antidepressive treatment, as exemplified by the attitude and approach adopted by the physician and by close reference persons towards...

Layden, J.; Michaels, J.; Bermingham, S.; Higgins, B.; Attwood, B.; Beech, A.; Bradbury, A.; Ettles, D.; Fox, M.; Flynn, M.; Lam, A.; Maufe, P.; Mullis, R.; Sharma, A.; Shearman, C.; Trender, H.; Uberoi, R.; Sharma, M.; Bermingham, S.; Cobb, J.; Higgins, B.; Kelley, K.; Krause, T.; Layden, J.; Parnham, J.; Sawyer, L.; Sparrow, K., 2012: Diagnosis and management of lower limb peripheral arterial disease: summary of NICE guidance. Bmj 345: E4947-E4947

Ohta M.; Yamashita M.; Egami H.; Takeshita I.; Kitamura K., 1982: Ultrastructural observation of hetero transplanted human glioma cells special reference to cell differentiation. Shinkei Kenkyu No Shinpo 26(3): 540-549

Preshaw, P.M., 2012: Critical issues in clinical periodontal research. As periodontal researchers and clinicians, we are challenged every day to make decisions relating to the clinical management of our patients and about how best to conduct clinical periodontal research. This volume of Periodontology 2000 addresses...

Prins B., 1987: Phylogenetic strategies in calcareous nannoplankton; not a black and white picture. INA Newsletter 9(2): 59

Anonymous, 1989: Neurobiology of incontinence. Lancet 2(8671): 1078-1079

Abidi, S.Hani.; Ahmed, K.; Sherwani, S.Khan.; Kazmi, S.Urooj., 2016: Synergy between antibiotics and natural agents results in increased antimicrobial activity against Staphylococcus epidermidis. Staphylococcus epidermidis is one of the most frequent causes of biofilm-associated infections on indwelling medical devices. With the emergence of methicillin-resistant S. epidermidis (MRSE), there is an urgent need to discover novel active agent...

Byrd, E.E.; Prestwood, A.K., 1970: A new genus and species, Psilotornus audacirrus Trematoda Digene a Psilostomatidae, from the wild turkey in Alabama. Trans. Am. microsc. Soc. 88: 1969: 366-369

Gelfand, M., 1965: Haematemesis in an african medical ward. Central African Journal of Medicine 11(12): 366-368