+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Continuous production of poly([R]-3-hydroxybutyrate) by Cupriavidus necator in a multistage bioreactor cascade

, : Continuous production of poly([R]-3-hydroxybutyrate) by Cupriavidus necator in a multistage bioreactor cascade. Applied Microbiology and Biotechnology 91(2): 295-304

Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes feature drawbacks such as varying product quality and the inevitable periods of downtime for preparation and post-treatment of the bioreactor equipment. An unprecedented approach to PHA production was chosen in the presented work using a multistage system consisting of five continuous stirred tank reactors in series (5-SCR), which can be considered as a process engineering substitute of a continuous tubular plug flow reactor. The first stage of the reactor cascade is the site of balanced bacterial growth; thereafter, the fermentation broth is continuously fed from the first into the subsequent reactors, where PHA accumulation takes place under nitrogen-limiting conditions. Cupriavidus necator was used as production strain. The focus of the experimental work was devoted to the development of a PHA production process characterized by high productivity and high intracellular polymer content. The results of the experimental work with the reactor cascade demonstrated its potential in terms of volumetric and specific productivity (1.85 g L⁻¹ h⁻¹ and 0.100 g g⁻¹ h⁻¹, respectively), polymer content (77%, w/w) and polymer properties (M (w) = 665 kg/mol, PDI = 2.6). Thus, implementing the technology for 5-SCR production of PHB results in an economically viable process. The study compares the outcome of the work with literature data from continuous two-stage PHA production and industrial PHA production in fed-batch mode.


PMID: 21503760

DOI: 10.1007/s00253-011-3260-0

Other references

Beavis, A., J.; Pennline, K., J., 1994: Tracking of murine spleen cells in vivo: Detection of PKH26-labeled cells in the pancreas of non-obese diabetic (NOD) mice. Flow cytometry was used to track the in vivo migration of PKH26-labeled donor spleen cells from diabetic NOD mice that were injected into non-diabetic recipient NOD mice. Flow cytometric analysis of recipient mouse tissues revealed that the donor...

Tsujino, S.; Ono, Y.; Yamamoto, S.; Tsuzuki, M.; Miki, M.; Aizawa, T.; Matsumoto, T.; Yamauchi, T., 1995: Clinical results of IV-compa (cisplatin, vincristine, methotrexate, peplomycin and adriamycin) chemotherapy for advanced urothelial cancer. We have developed a new combination intravenous chemotherapy regimen called COMPA (IV-COMPA). The clinical value of IV-COMPA chemotherapy was evaluated based on the results of 24 patients with urothelial cancers. From October 1989 through October...

Currier, N.L.; Lejtenyi, D.; Miller, S.C., 2003: Effect over time of in-vivo administration of the polysaccharide arabinogalactan on immune and hemopoietic cell lineages in murine spleen and bone marrow. Current evidence indicates an immunostimulating role for complex carbohydrates, i.e., polysaccharides, from several plant sources. In the present work, we determined the specific in vivo effects, with time of administration, of one such compound,...

Reisz, G., 1966: A rapid method for the determination of serum beta-lipoproteins. Zeitschrift für Medizinische Labortechnik 7(1): 35-39

Yang, Z.; Guo, G.; Zhang, M.; Liu, C.Y.; Hu, Q.; Lam, H.; Cheng, H.; Xue, Y.; Li, J.; Li, N., 2014: Stable isotope metabolic labeling-based quantitative phosphoproteomic analysis of Arabidopsis mutants reveals ethylene-regulated time-dependent phosphoproteins and putative substrates of constitutive triple response 1 kinase. Ethylene is an important plant hormone that regulates numerous cellular processes and stress responses. The mode of action of ethylene is both dose- and time-dependent. Protein phosphorylation plays a key role in ethylene signaling, which is media...

Hedriana, H.L.; Moore, T.R., 1994: Accuracy limits of ultrasonographic estimation of human fetal urinary flow rate. OBJECTIVE: Our purpose was to define the accuracy of currently available methods of ultrasonographically estimating human fetal urinary flow rate in a controlled setting. STUDY DESIGN: Eleven fetal cadavers were studied in a water bath. Saline sol...

Woolverton W.L.; Kamien J.B.; Goldberg L.I., 1985: Effects of selective dopamine receptor agonists in rats trained to discriminate apomorphine from saline. Rats (N = 12) were trained to discriminate apomorphine (0.25 mg/kg i.p.) from saline in a 2 lever, food-reinforced (FR 30) drug discrimination paradigm. When the discrimination was acquired, various doses of apomorphine as well as several other do...

Stepanov, V.M., 1982: Microheterogeneity of proteins. Uspekhi Sovremennoi Biologii 93(1): 35-45

Joormann, J.; Gotlib, I.H., 2008: Updating the contents of working memory in depression: interference from irrelevant negative material. This study was designed to assess the effects of irrelevant emotional material on the ability to update the contents of working memory in depression. For each trial. participants were required to memorize 2 lists of emotional words and subsequentl...

Tekkok, S.B.; Godfraind, J.M.; Krnjevic, K., 1998: 2-Deoxy-D-glucose -induced LTP is impaired in hippocampal slices from mdx and mdx-3CV mice. Society for Neuroscience Abstracts 24(1-2): 1168