geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Control of dendritic cell migration, T cell-dependent immunity, and autoimmunity by protein tyrosine phosphatase PTPN12 expressed in dendritic cells


, : Control of dendritic cell migration, T cell-dependent immunity, and autoimmunity by protein tyrosine phosphatase PTPN12 expressed in dendritic cells. Molecular and Cellular Biology 34(5): 888-899

Dendritic cells (DCs) capture and process antigens in peripheral tissues, migrate to lymphoid tissues, and present the antigens to T cells. PTPN12, also known as PTP-PEST, is an intracellular protein tyrosine phosphatase (PTP) involved in cell-cell and cell-substratum interactions. Herein, we examined the role of PTPN12 in DCs, using a genetically engineered mouse lacking PTPN12 in DCs. Our data indicated that PTPN12 was not necessary for DC differentiation, DC maturation, or cytokine production in response to inflammatory stimuli. However, it was needed for full induction of T cell-dependent immune responses in vivo. This function largely correlated with the need of PTPN12 for DC migration from peripheral sites to secondary lymphoid tissues. Loss of PTPN12 in DCs resulted in hyperphosphorylation of the protein tyrosine kinase Pyk2 and its substrate, the adaptor paxillin. Pharmacological inhibition of Pyk2 or downregulation of Pyk2 expression also compromised DC migration, suggesting that Pyk2 deregulation played a pivotal role in the migration defect caused by PTPN12 deficiency. Together, these findings identified PTPN12 as a key regulator in the ability of DCs to induce antigen-induced T cell responses. This is due primarily to the role of PTPN12 in DC migration from peripheral sites to secondary lymphoid organs through regulation of Pyk2.

US$19.90

PMID: 24366546

DOI: 10.1128/MCB.01369-13


Other references

Guzzetti Fausto; Reichenbach Paolo; Cardinali Mauro; Ardizzone Francesca; Stark Colin P.; Taramelli Andrea, 2004: Towards a national classification of landslide and flood hazards and risk in Italy. International Geological Congress, Abstracts = Congres Geologique International, Resumes 32, Part 1: 79

Guo, T.; Baasner, J., 1993: On-line microwave sample pretreatment for the determination of mercury in blood by flow injection cold vapor atomic absorption spectrometry. A method for on-line treatment of whole blood in a microwave oven and determination of mercury by flow injection cold vapor atomic absorption spectrometry was developed. After dilution of the whole blood and addition of oxidant, all further treatm...

Chauhan, B.C.; Vesti, E.; Johnson, C.A., 2002: Comparison of Methods for Detecting Glaucomatous Visual Field Progression. Purpose: To compare the performance characteristics of 7 criteria for analyzing glaucomatous visual field progression, using a combination of real patient data and computer simulation. Methods: Initial and final Humphrey 30-2 visual fields (separa...

Pereira, L.Augusto.Pinto.; Groppo, F.Carlos.; Bergamaschi, C.de.Cássia.; Meechan, J.Gerard.; Ramacciato, J.Cama.; Motta, Rério.Heládio.Lopes.; Ranali, Jé., 2014: Articaine (4%) with epinephrine (1:100,000 or 1:200,000) in intraosseous injections in symptomatic irreversible pulpitis of mandibular molars: anesthetic efficacy and cardiovascular effects. The aim of this study was to compare the cardiovascular effects and the anesthetic efficacy of intraosseous injections of 4% articaine with 1:100,000 epinephrine (EPI100) or 4% articaine with 1:200,000 epinephrine (EPI200). In this prospective, ra...

Xu, S.M.; Peng, S.Y.; Chen, P.H., 1994: Glucagonoma syndrome report of one case and review of the chinese literature. This paper reports one case with glucagonoma syndrome that resection of the primary glucagonoma in the pancreas was done. There was metastasis one in liver and lymph node. The definitive diagnosis was established by immunohistochemical analysis. T...

Oh, Y-Sang.; Ma, H-Il.; Kim, Y-Joong.; Kim, J-Seok., 2012: Task-specific tremor with use of scissors. Movement Disorders 27(7): 921-922

Grimm Robert E.; Phillips Roger J., 1990: Venusian gravity and tectonics on the brink of Magellan. Abstracts with Programs - Geological Society of America Pages 127

Bauer, R., 1996: The enchytraeid fauna Annelida; Oligochaeta along an altitudinal gradient in Gasteiner Tal Salzburg and description of a new species Fridericia antensteineri n sp Die Enchytraeidenfauna Annelida; Oligochaeta entlang eines Hohengradienten im Gasteiner Tal Salzburg und Beschreibung der neuen Art Fridericia antensteineri n sp. The composition of the enchytraeid fauna in a spruce forest and a subalpine meadow along an altitudinal gradient from 830 to 2.030 m a.s.l. in the "Gasteiner Tal" was investigated. Altogether 20 species were found, one of them being new...

Stote R.M.; Alexander F.; Familiar R.G.; Hoppe R.C., 1974: Effect of triamterene on furosemide induced kaliuresis in normal volunteers. Journal of International Medical Research 2(6): 379-383

Bréhélin, L.; Florent, I.; Gascuel, O.; Maréchal, E., 2010: Assessing functional annotation transfers with inter-species conserved coexpression: application to Plasmodium falciparum. Background: <i>Plasmodium falciparum</i> is the main causative agent of malaria. Of the 5 484 predicted genes of <i>P. falciparum</i>, about 57% do not have sufficient sequence similarity to characterized genes in other spe...