geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cooperative folding of tau peptide by coordination of group IIB metal cations during heparin-induced aggregation


, : Cooperative folding of tau peptide by coordination of group IIB metal cations during heparin-induced aggregation. Biometals 25(2): 361-372

The group IIB elements, especially Cd(II) and Hg(II), are increasingly considered as potential environmental neurotoxins. This study demonstrates that the Alzheimer's tau fragment R2, corresponding to the second repeat of the microtubule-binding domain, can bind to Zn(II), Cd(II) and Hg(II). Isothermal titration calorimetry experiments suggest that the most likely coordination site is the thiol group of Cys291, and this is further confirmed by a control experiment using a C291A mutant peptide. Circular dichroism spectrum reveals that the coordination of group IIB cations, especially Hg(II), can induce pronounced conformational conversions in natively unfolded R2, from random coil to other ordered structures. ThS fluorescence assays and electron microscopy indicate that the group IIB cations promote heparin-induced aggregation of R2, giving relatively small R2 filaments. The efficiency in promoting aggregation, as well as inducing conformational conversion, varies strongly with the cation's polarizability. Based on these results, a model is proposed in which the cooperative folding of R2 through cross-bridging of group IIB cations is suggested to be a key factor in promoting aggregation, in addition to the effective neutralization of coulombic charge-charge repulsion by heparin, the poly-anion inducer. Our results provide clues to understanding the potential pathogenic role of group IIB metals in the development of neurofibrillary tangles, a typical hallmark of Alzheimer's disease.

US$19.90

PMID: 22083130

DOI: 10.1007/s10534-011-9505-7


Other references

Von Bonin A.; Martin S.; Weltzien H.U., 1991: Covalent or peptide mediated mhc modification relevance in hapten specific t cell responses. Immunobiology 183(3-4): 334-335

Sands, W.A.; Kusel, J.R., 1992: Changes in the lateral diffusion of fluorescent lipid analogues in the surface membrane of adult male Schistosoma mansoni. The effect of serotonin on the fluidity of the tegumental membranes of adult male Schistosoma mansoni was assessed by the fluorescence recovery after photobleaching technique. It was demonstrated that the translational diffusion of 5-N'-octad...

Maluka, S.; Kamuzora, P.; San Sebastiån, M.; Byskov, J.; Olsen, Øystein.E.; Shayo, E.; Ndawi, B.; Hurtig, A-Karin., 2010: Decentralized health care priority-setting in Tanzania: evaluating against the accountability for reasonableness framework. Priority-setting has become one of the biggest challenges faced by health decision-makers worldwide. Fairness is a key goal of priority-setting and Accountability for Reasonableness has emerged as a guiding framework for fair priority-setting. Thi...

Huang, D.Wei.; Sherman, B.T.; Tan, Q.; Kir, J.; Liu, D.; Bryant, D.; Guo, Y.; Stephens, R.; Baseler, M.W.; Lane, H.Clifford.; Lempicki, R.A., 2007: DAVID Bioinformatics Resources: expanded annotation database and novel algorithms to better extract biology from large gene lists. All tools in the DAVID Bioinformatics Resources aim to provide functional interpretation of large lists of genes derived from genomic studies. The newly updated DAVID Bioinformatics Resources consists of the DAVID Knowledgebase and five integrate...

Song, B.; Liu, J.; Feng, X.; Wei, L.; Shao, L., 2015: A review on potential neurotoxicity of titanium dioxide nanoparticles. As the rapid development of nanotechnology in the past three decades, titanium dioxide nanoparticles (TiO2 NPs), for their peculiar physicochemical properties, are widely applied in consumer products, food additives, cosmetics, drug carriers, and...

Chris Noble; Sunil Kadri; David F.M.tchell; Felicity A.H.ntingford, 2008: Growth, production and fin damage in cage-held 0+ Atlantic salmon pre-smolts (Salmo salar L.) fed either a) on-demand, or b) to a fixed satiation-restriction regime: Data from a commercial farm. This experiment investigated the impact of feeding regime upon the growth performance, production efficiency and fin damage of cage-held 0+ Atlantic salmon pre-smolts. Six groups of pre-smolts (n= 61847 +/- 2620 fish group(-1)) were held in six 1...

Toda, N.; West, T.C., 1965: Changes in sino-atrial node transmembrane potentials on vagal stimulation of isolated rabbit atrium. Nature 205(4973): 808-809

Nikkarinen, M.; Kauniskangas, E.; Nenonen, K., 1996: Use of geological and geochemical data for the derivation of maps for environmental planning in Iisalmi, Finland. Geoscientific maps for the purposes of land-use planning are being produced, the pilot area for this being the town of Iisalmi in Eastern Finland. The collection comprises thematic maps of natural resources, water reserves, susceptibility to acidi...

León Vintró, L.; Mitchell, P.I.; Omarova, A.; Burkitbayev, M.; Jiménez Nápoles, H.; Priest, N.D., 2009: Americium, plutonium and uranium contamination and speciation in well waters, streams and atomic lakes in the Sarzhal region of the Semipalatinsk Nuclear Test Site, Kazakhstan. New data are reported on the concentrations, isotopic composition and speciation of americium, plutonium and uranium in surface and ground waters in the Sarzhal region of the Semipalatinsk Test Site, and an adjacent area including the settlement o...

Gambardella, P.; Dallmeyer, A.; Maiti, K.; Malagoli, M.C.; Rusponi, S.; Ohresser, P.; Eberhardt, W.; Carbone, C.; Kern, K., 2004: Oscillatory magnetic anisotropy in one-dimensional atomic wires. One-dimensional Co atomic wires grown on Pt(997) have been investigated by x-ray magnetic circular dichroism. Strong changes of the magnetic properties are observed as the system evolves from 1D- to 2D-like. The easy axis of magnetization, the mag...