geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase δ


, : Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase δ. Journal of Biological Chemistry 286(45): 39546-39559

Perturbation of cell cycle regulation is a characteristic feature of infection by many DNA and RNA viruses, including Coronavirus infectious bronchitis virus (IBV). IBV infection was shown to induce cell cycle arrest at both S and G(2)/M phases for the enhancement of viral replication and progeny production. However, the underlying mechanisms are not well explored. In this study we show that activation of cellular DNA damage response is one of the mechanisms exploited by Coronavirus to induce cell cycle arrest. An ATR-dependent cellular DNA damage response was shown to be activated by IBV infection. Suppression of the ATR kinase activity by chemical inhibitors and siRNA-mediated knockdown of ATR reduced the IBV-induced ATR signaling and inhibited the replication of IBV. Furthermore, yeast two-hybrid screens and subsequent biochemical and functional studies demonstrated that interaction between Coronavirus nsp13 and DNA polymerase δ induced DNA replication stress in IBV-infected cells. These findings indicate that the ATR signaling activated by IBV replication contributes to the IBV-induced S-phase arrest and is required for efficient IBV replication and progeny production.

US$19.90

PMID: 21918226

DOI: 10.1074/jbc.M111.242206


Other references

Smith, L.C.; Sinclair, A.G., 1998: Effects and interactions of P fertiliser forms and rates of lime on a clover/ryegrass sward. Triple superphosphate (TSP), Arad phosphate rock (APR), and "Longlife" superphosphate (LL) were compared for their effectiveness as P fertilisers on a ryegrass/clover sward in New Zealand to which lime had been applied at 0, 1.25, 2.5, a...

Sturgill T.W.; Ray L.B.; Erikson E.; Maller J.L., 1988: Insulin stimulated map 2 kinase phosphorylates and activates ribosomal protein s6 kinase ii. Nature (London) 334(6184): 715-718

Peyrottes, S.; Coussot, Gëlle.; Lefebvre, I.; Imbach, J-Louis.; Gosselin, G.; Aubertin, A-Marie.; Périgaud, C., 2003: S-acyl-2-thioethyl aryl phosphotriester derivatives of AZT: synthesis, antiviral activity, and stability study. The synthesis, antiviral activity, and stability study of phosphotriester derivatives of 3'-azido-2',3'-dideoxythymidine (AZT) bearing modified l-tyrosinyl residues are reported. These compounds were obtained via phosphoramidite (P(...

Palmer, R.S.; Nyssens, A.F.; White, J.C., 1948: Severe hypertension with papilledema simulating brain tumor; differential diagnosis and treatment. New England Journal of Medicine 239(9): 322-327

Feer, F., 1979: Observations ecologiques sur le neotrague de Bates (Neotragus batesi de Winton, 1903, artiodactyle, ruminant, bovide) du nord-est du Gabon. Bates's pygmy antelope N. batesi was studied for 15 mo. in north east Gabon. Animals (14) were individually marked and 6 (5 .female..female. and 1 .lambda.) were monitored by radio-tracking. During the same period, 128 specimens were collecte...

Landers, S.; Berry, P.L.; Kearns, G.L.; Kaplan, S.L.; Rudolph, A.J., 1984: Gentamicin disposition and effect on development of renal function in the very low birth weight infant. The steady-state pharmacokinetics, renal function and quantitative beta 2-microglobulin (beta 2-M) excretion were prospectively evaluated in 22 very low birth weight (VLBW) infants (700-1,470 g birth weight and 25-33 weeks gestational age) receivi...

Preisman, A.B.; Maksimovich, A.Bodik, N.M., 1963: Nestor Maksimovich Maksimovich-Ambodik (On The 150th Anniversary Of His Death). Akusherstvo i Ginekologiia 39: 137-140

Kanazawa, H.; Hirata, K.; Yoshikawa, J., 2002: Nitrogen oxides reduce albuterol-induced bronchodilation in patients with bronchial asthma. We have previously found that asthmatics exhibit higher levels of nitrogen oxides (NOs) in exhaled air and in induced sputum than normal controls, and that NOs altered beta(2)-adrenoceptor (beta(2)-AR) function in an experimental animal model. To...

Vohra, R.S.; Mathur, S.; Bryant, N.D.; Forbes, S.C.; Vandenborne, K.; Walter, G.A., 2016: Age-related T2 changes in hindlimb muscles of mdx mice. Magnetic resonance imaging (MRI) was used to monitor changes in the transverse relaxation time constant (T2) in lower hindlimb muscles of mdx mice at different ages. Young (5 weeks), adult (44 weeks), and old mdx (96 weeks), and age-matched contro...

Gurnani, M., 1985: Constraints in the improvement of milk production in farmers conditions. Improvements in public relations, management and recording at farm level, and scientific techniques necessary for the effective running of progeny testing schemes for dairy bulls in India are outlined.