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Correlative assessment of fecal indicators using human mitochondrial DNA as a direct marker


, : Correlative assessment of fecal indicators using human mitochondrial DNA as a direct marker. Environmental Science & Technology 47(18): 10485-10493

Identifying the source of surface water fecal contamination is paramount to mitigating pollution and risk to human health. Fecal bacteria such as E. coli have been staple indicator organisms for over a century, however there remains uncertainty with E. coli-based metrics since these bacteria are abundant in the environment. The relationships between the presence of direct indicator of human waste (human mitochondrial DNA), human-specific Bacteroidales, and E. coli were studied for water samples taken from an urban creek system (Duck Creek Watershed, Cincinnati, OH) impacted by combined sewer overflows. Logistic regression analysis shows that human-specific Bacteroidales correlates much more closely to human mitochondrial DNA (R = 0.62) relative to E. coli (R = 0.33). We also examine the speciation of Bacteroidales within the Duck Creek Watershed using next-generation sequencing technology (Ion Torrent) and show the most numerous populations to be associated with sewage. Here we demonstrate that human-specific Bacteroidales closely follow the dynamics of human mitochondrial DNA concentration changes, indicating that these obligate anaerobes are more accurate than E. coli for fecal source tracking, lending further support to risk overestimation using coliforms, especially fecal coliforms and E. coli.

US$19.90

PMID: 23919424

DOI: 10.1021/es4020458


Other references

Ranson, G., 1926: La nutrition chez les animaux aquatiques. CR Acad Sci Paris, 182: 1102-1104

Xue, B.; Kahn, B.B., 2006: AMPK integrates nutrient and hormonal signals to regulate food intake and energy balance through effects in the hypothalamus and peripheral tissues. The evolutionarily conserved serine/threonine kinase, AMP-activated protein kinase (AMPK), functions as a cellular fuel gauge that regulates metabolic pathways in glucose and fatty acid metabolism and protein synthesis. Recent data strongly implic...

Loaiciga Hugo A., 2002: Sustainable ground-water exploitation. Principles of sustainable ground-water exploitation are presented in this paper. The renewable and inappropriable nature of ground water is examined in light of the process of recharge. An example illustrates the interplay among ground-water extra...

D.Carlo F.J., 1984: Symposium on safer chemicals through molecular design part 1 arlington virginia usa sept 11 15 1983. Drug Metabolism Reviews: 409-646

Ziegenfuss, MC.; Hall, L W.; Jr.; Bushong, SJ.; Sullivan, JA.; Unger, MA., 1990: Remote in situ apparatus for ambient toxicity testing of larval and yearling fish in river or estuarine systems. A remote in situ apparatus for ambient toxicity testing of prolarval and yearling striped bass, Morone saxatilis, was designed and tested in the upper Chesapeake Bay and Potomac River during the 1989 spawning season. The apparatus' design, ca...

Schweisheimer, W., 1962: Lactic dehydrogenase in the diagnosis of cancer and myocardial disease. Enzymes as diagnostic aids. Die Medizinische Welt 46: 2467-2468

Leatherman, S.T.; Hibbard, J.H.; McGlynn, E.A., 2003: A research agenda to advance quality measurement and improvement. In developing a conceptual framework for the design of a national quality measurement and reporting system (NQMRS), the Strategic Framework Board (SFB) recommends that such a system be built on a strong evidence base. To identify critical gaps in...

Scott D.H.; Moore H.J.; Hodges C.A., 1974: Multiringed basins. Pages 695-697 1974

Ortiz, J.M.; Garcia Lidon, A., 1982: Lemon rootstocks. The six main rootstocks used for lemons in Spain are described. Less commonly used rootstocks briefly described include a natural hybrid, recently noticed and propagated under the designation RLO at La Alberca, and some selected lines of bitter or...

Naqvi S.M., 1981: The oldest supracrustals of the Dharwar Craton, India. Journal of the Geological Society of India 22(10): 458-469