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Creating 3D angiogenic growth factor gradients in fibrous constructs to guide fast angiogenesis


, : Creating 3D angiogenic growth factor gradients in fibrous constructs to guide fast angiogenesis. Biomacromolecules 13(10): 3262-3271

Fast angiogenesis in 3D fibrous constructs that mimic the morphology of the extracellular matrix remains challenging due to limited porosity in the densely packed constructs. We investigated whether mimicking the in vivo chemotaxis microenvironment for native blood vessel formation would stimulate angiogenesis in the fibrous constructs. The chemotaxis microenvironment was created by introducing 3D angiogenic growth factor gradients into the constructs. We have developed a technique that can quickly fabricate (∼40 min) such 3D gradients by simultaneously electrospinning polycaprolactone (PCL) fibers, encapsulating gradient amount of bFGF (stabilized by heparin) into poly(lactide-co-glycolide) (PLGA) microspheres, and electrospraying the microspheres into PCL fibers. Gradient formation was confirmed by fluorescence microscopy. Gradients with different steepnesses were obtained by modulating the initial concentration of the bFGF solution. All of the constructs were able to sustainedly release bioactive bFGF over a 28 day period. The release kinetics was dependent on the bFGF loading and steepness of the gradient. In vitro cell migration study demonstrated that bFGF gradients significantly increased the depth of cell migration. To assess the efficacy of bFGF gradients in inducing angiogenesis, we implanted constructs subcutaneously using mouse model. bFGF gradients significantly promoted cell penetration into the constructs. After 10 days of implantation, a high density of mature blood vessels (positive to both CD31 and α-SMA) were formed in the constructs. Vessel density was increased with the increase in steepness of the bFGF gradient. These gradient constructs may have potential to engineer vascularized tissues for various applications.

US$19.90

PMID: 22924876

DOI: 10.1021/bm301029a


Other references

Davison, F.R.; Willaman, J.J., 1927: Biochemistry of plant diseases. IX. Pectic enzymes. Very little is known concerning the number and nature of the pectic enzymes. Protopectinase (pectosinase) was prepared from Rhizopus tritici and Bacillus carotovorus[long dash]in the former both the intracellular and extracellular, and in the latt...

Guillet, Séphanie.; Hirsch, P.; Marzac, C.; Mohty, M.; Marie, J-Pierre.; Legrand, O., 2015: Long-term imatinib maintenance therapy for adult Philadelphia positive acute lymphoblastic leukemia. Leukemia & Lymphoma 55(11): 2646-2648

Ishikawa, H.; Kanaehiro, H.; Nakano, H.; Kanaizumi, T., 1997: Measurement of gastric emptying using radioisotope. Nihon Rinsho. Japanese Journal of Clinical Medicine 55 Suppl 2: 139-142

Ravina J.H.; Poulain M.; Lepage F., 1969: Prevention and control of rhesus iso immunization. Presse Medicale 77(43): 1511-1513

Saiduzzafar, H.; Ansari, Z.; Kumar, M., 1978: Wilson's disease with special reference to ocular manifestations (a case report). Indian Journal of Ophthalmology 26(2): 37-39

Kudernatsch, F., 1965: Lepidopteren-Fauna von Gloggnitz und Umgebung. Erganzung des Prodromus, Zone 6. Zeitschrift der Wiener Entomologischen Gesellschaft, 50: 209-222

Chevrel, J.P.; Duclos, J.P.; Gutel, C.; Rouvillois, C., 1972: Spontaneous ruptures of the esophagus (apropos of 4 cases). Medecine & Chirurgie Digestives 1(5): 265-270

Sarkar, C.; Chand Sharma, M.; Nayak, A.; Mercy Ralte, A.; Gupta, V.; Singh, S.; Behari, M., 2005: Primary AL (kappa-light chain) amyloidosis manifesting as peripheral neuropathy in a young male without increase in serum and urine immunoglobulin load: a diagnostic challenge. Primary systemic or AL amyloidosis is a multisystem disorder characterized by diffuse extracellular infiltration of a fibrillar protein of monoclonal light chain origin (AL). Majority of the patients have monoclonal immunoglobulin in serum and/or...

Takanami, T.; Takahashi, H.; Abe, T.; Yoshida, S.; Higashitani, A., 2002: Repair systems for DNA damage by heavy-ion irradiation in meiotic cells. Uchu Seibutsu Kagaku 15(3): 274-275

Henry Darrel; Dutrow Barbara, 2003: Taos Plateau volcanic project; a vehicle for integration of concepts in igneous petrology. Eos, Transactions, American Geophysical Union 84(46, Suppl