geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells


, : Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells. Veterinary Microbiology 144(1-2): 51-57

Cholesterol is involved in the life cycle of many viruses. Here, we examined the role of cholesterol for both viral envelope and target cell membrane for bovine herpesvirus type 1 (BoHV-1) infection. Cholesterol depletion by pretreatment of Madin-Darby bovine kidney (MDBK) cells with a cholesterol-sequestering drug methyl-beta-cyclodextrin (MbetaCD), inhibited the production of BoHV-1 in a dose-dependent manner. This inhibitory effect was partially reversed by cholesterol replenishment, indicating that the reduction was caused by cholesterol depletion. Cholesterol depletion at the post-entry stage only had a mild effect on the virus production. However, cell membrane cholesterol depletion did not reduce the virus attachment. In addition, treatment of BoHV-1 particles with MbetaCD also reduced the virus infectivity significantly and the effect was partially reversed by addition of exogenous cholesterol. Taken together, these data implicated that cell membrane cholesterol mainly contributed to BoHV-1 entry into MDBK cells and the viral envelope cholesterol was also essential for the virus infectivity.

US$19.90

PMID: 20097021

DOI: 10.1016/j.vetmic.2009.12.031


Other references

Anonymous, 1977: Projections of demand for sawn timbers in Brazil 1975-2000. The procedure adopted in making the calculations is explained, and data on the roundwood equivalent of the estimated future demand for sawn timbers are presented in tables and graphs.

Haghparast, H.; Ghorbani, A.; Rohlin, M., 2016: Dental students' perception of their approaches to learning in a PBL programme. To compare dental students' perceptions of their learning approaches between different years of a problem-based learning (PBL) programme. The hypothesis was that in a comparison between senior and junior students, the senior students would pe...

King, S.A., 1994: Acts of compassion: a response to Dr. Eddy's and Dr. Massey's articles. Connecticut Medicine 58(11): 696-697

Martel, P.; Connan, L.; Bonnet, F.; Delannes, M.; Farnarier, J.; Mihura, J.; El-Ghaoui, A., 2000: Aspects diagnostiques, pronostiques et therapeutiques des adenocarcinomes du col uterin: A propos d'une serie de 49 cas (Etude cas-temoin). Objectives: The aim of this study was to search for potential diagnostic, therapeutic and prognosis differences between a series of 49 adenocarcinomas of the cervix and a matched series of epidermoid carcinomas. Methods: Forty-nine adenocarcinomas...

Smith, S.C.; Joshi, K.K.; Zik, J.J.; Trinh, K.; Kamajaya, A.; Chien, P.; Ryan, K.R., 2015: Cell cycle-dependent adaptor complex for ClpXP-mediated proteolysis directly integrates phosphorylation and second messenger signals. The cell-division cycle of Caulobacter crescentus depends on periodic activation and deactivation of the essential response regulator CtrA. Although CtrA is critical for transcription during some parts of the cell cycle, its activity must be elimi...

Gayda, S.; Nienhaus, K.; Nienhaus, G.Ulrich., 2013: Mechanistic insights into reversible photoactivation in proteins of the GFP family. Light-controlled modification of the fluorescence emission properties of proteins of the GFP family is of crucial importance for many imaging applications including superresolution microscopy. Here, we have studied the reversibly photoswitchable f...

Alpern, Z.; Ells, P.F.; Pollack, B.J.; Brand, D.L.; Birk, J.W.; Anderson, J.C., 2000: Tobacco and colorectal polyps 1 cm Is there a smoking gun?. Gastroenterology 118(4 Suppl 2 Part 2): AGA A1077, April

Wiborg, K.F., 1948: Investigations on cod larvae in the coastal waters of northern Norway Occurrence of cod larvae, and occurrence of food organisms in the stomach contents and in the sea. The occurrence of cod (Gadus callarias) eggs and larvae, the contents of the stomachs of some of the larvae, and the quantity of food organisms in the sea have been studied. The material was collected in plankton samples in 1930, 1933, 1939, 1946,...

Frazzetto, S., 1930: Action of some anions on the tonus and automatic movements of the frogs stomach. The anions of salicylate, benzoate, cyanide and tartrate exercise an inhibiting action on tonus and automatic movements of frog's stomach. For all but tartrate an exciting action may precede the stage of inhibition.

Swiderska, K.; Swiderski, G., 1978: Studies on the types of transitional lumbosacral vertebrae in children. Polski Przeglad Radiologii i Medycyny Nuklearnej 42(2): 131-134