geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.)


, : Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.). Cryo Letters 29(4): 339-350

This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20 % which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.

US$29.90

PMID: 19137197


Other references

Saito, H.; Tomioka, H.; Watanabe, T.; Yoneyama, T., 1983: Mycobacteriocins produced by rapidly growing mycobacteria are Tween-hydrolyzing esterases. Smegmatocin, a protein produced by Mycobacterium smegmatis ATCC 14468, was found to have an esterase activity, hydrolyzing Tween 80, polyoxyethylene sorbitan monooleate, added to the assay medium for various "bacteriocins" from mycobacte...

Luosto, R.; Harjola, P.T.; Ketonen, P.; Tala, P., 1976: Operative treatment of atherosclerotic lesions in innominate, subclavian and vertebral arteries. A follow-up study. The follow-up study consists of 121 surgically treated atherosclerotic patients. The average age was 49 years and the male: female ratio 7:5. There were 165 obstructions or occlusions in this series. 59 of them suffered from a subclavian steal syn...

Yamamoto H.; Kanaide H., 1991: Potassium ions modulate uptake and release of calcium in the intracellular stores of vascular smooth muscle cells. Circulation 84(4 SUPPL 2): II171

Halbrendt J.M.; Podleckis E.V.; Hadidi A.; Scorza R.; Welliver R.A., 1991: Evaluation of prunus for resistance to natural infection of tomato ringspot virus. Hortscience 26(6): 748

Eiberle, K., 1976: The use of artificial improvement of grazing. Feld Wald Wasser Schweizerische Jagdzeitung: 4 (11) 8

Seitz, G.J.; Haeussler, P.J.; Crone, A.J.; Lipovsky, P.; Schwartz, D.P., 2008: Eastern Denali Fault slip rate and paleoseismic history, Kluane Lake area, Yukon Territory, Canada. Eos, Transactions, American Geophysical Union 89.53, Suppl.

Scott Maryann; Herman Gregory C., 1998: Topographic bitmap images for Sussex, Somerset, Cumberland, Hunterdon, Warren, Morris, and Passaic counties, New Jersey. Open-File Report - U S

Redpath Mahon, A.; Neu, A.M., 2016: A contemporary approach to the prevention of peritoneal dialysis-related peritonitis in children: the role of improvement science. NlmCategory="UNASSIGNED">Peritonitis is a leading cause of hospitalizations, morbidity, and modality change in pediatric chronic peritoneal dialysis (CPD) patients. Despite guidelines published by the International Society for Periton...

Haury, V.G.; Gruber, C.M.; Gruber, C.M., 1939: The effect of intravenous injections of thiobarbiturates on the volumes of the spleen and kidney. Thiobarbiturates produce pressor effects and a decrease in vol. of spleen and kidney, when injected intraven. in intact or spinal dogs. The effects of evipal are opposite. Vascular effects of the thiobarbiturates are partly peripheral.

Abdel Mohsen, M.M.; Bauer, J., 2001: Cenomanian ostracode faunas from Sinai Peninsula, Egypt. Detailed study of the ostracode faunas from the Cenomanian sequence of Sinai yielded 39 ostracode species belonging to 22 genera. Out of these, six species namely Cytherella dhalalensis, Cytherella gunnaensis, Neocyprideis boukharyi, Sarlatina bab...