+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Crystal structure of Arabidopsis thaliana Dawdle forkhead-associated domain reveals a conserved phospho-threonine recognition cleft for dicer-like 1 binding

, : Crystal structure of Arabidopsis thaliana Dawdle forkhead-associated domain reveals a conserved phospho-threonine recognition cleft for dicer-like 1 binding. Molecular Plant 6(4): 1290-1300

Dawdle (DDL) is a microRNA processing protein essential for the development of Arabidopsis. DDL contains a putative nuclear localization signal at its amino-terminus and forkhead-associated (FHA) domain at the carboxyl-terminus. Here, we report the crystal structure of the FHA domain of Arabidopsis Dawdle, determined by multiple-wavelength anomalous dispersion method at 1.7-Å resolution. DDL FHA structure displays a seven-stranded β-sandwich architecture that contains a unique structural motif comprising two long anti-parallel strands. Strikingly, crystal packing of the DDL FHA domain reveals that a glutamate residue from the symmetry-related DDL FHA domain, a structural mimic of the phospho-threonine, is specifically recognized by the structurally conserved phospho-threonine binding cleft. Consistently with the structural observations, co-immuno-precipitation experiments performed in Nicotiana benthamiana show that the DDL FHA domain co-immuno-precipitates with DCL1 fragments containing the predicted pThr+3(Ile/Val/Leu/Asp) motif. Taken together, we count the recognition of the target residue by the canonical binding cleft of the DDL FHA domain as the key molecular event to instate FHA domain-mediated protein-protein interaction in plant miRNA processing.


PMID: 23313986

DOI: 10.1093/mp/sst007

Other references

Anaclerio, A.M.; Wicker, H.S., 1970: Self-induced solar retinopathy by patients in a psychiatric hospital. American Journal of Ophthalmology 69(5): 731-736

Reisin E.; Wilson J.R., 1981: Increased pressor reactivity in ventromedial hypothalamic lesion induced obesity. The influence of ventromedial hypothalamic (VMH) lesion-induced obesity on basal hemodynamics and pressor responsiveness to cardioventricular infusions of l-norepinephrine (NE) was assessed in female rats. No group differences in basal tail systol...

Gleiter C.H.; Wozniak K.M.; Dejong J., 1989: Chronic antidepressant drug ad treatment attenuates hypothermia caused by electroconvulsive shock ecs. Society for Neuroscience Abstracts 15(1): 853

Beattie, J.R.; Bell, S.E.J.; Moss, B.W., 2004: A critical evaluation of Raman spectroscopy for the analysis of lipids: fatty acid methyl esters. The work presented here is aimed at determining the potential and limitations of Raman spectroscopy for fat analysis by carrying out a systematic investigation of C4-C24 FAME. These provide a simple, well-characterized set of compounds in which th...

Oyola-García, A.; Lituma-Aguirre, D.; Honorio-Morales, H., 2015: Financing rare or orphan diseases. Revista Peruana de Medicina Experimental Y Salud Publica 31(4): 808-809

Rojas C.; Mcfarland J.T., 1986: Nitrofurylacryloyl dipalmitoyltriacylglyceride as a substrate for lipoprotein lipase. Federation Proceedings 45(6): 1675

Barrero, Mía.J.; Malik, S., 2006: Two functional modes of a nuclear receptor-recruited arginine methyltransferase in transcriptional activation. Nuclear receptors, like other transcriptional activators, switch on gene transcription by recruiting a complex network of coregulatory proteins. Here, we have identified the arginine methyltransferase PRMT1 as a coactivator for HNF4, an orphan nuc...

Buslov M.M.; Sintubin M., 1995: Structural evolution of the Lake Teletskoe Zone, Altai-Sayan folded area. Russian Geology and Geophysics 36(10): 81-87

Tien, S.F., 1990: Quality assurance application in nursing practice--experience of Mackay Memorial Hospital. Hu Li Za Zhi Journal of Nursing 37(1): 39-45

Flamme, A.; Van Overbeke, H.; D.W.tte, J.; Decat, M.; Muysoms, F.; Claeys, D., 1995: Technical note: the use of arterialized arm veins in infrainguinal bypass. In order to augment the vein donation area of arm veins for infrainguinal bypass, we performed an arteriovenous AV fistula prior to the revascularization procedure. This technique was used in 5 patients. The diameter enlargement of the vein obtain...