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Crystal structure of an RNA polymerase ribozyme in complex with an antibody fragment

, : Crystal structure of an RNA polymerase ribozyme in complex with an antibody fragment. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 366(1580): 2918-2928

All models of the RNA world era invoke the presence of ribozymes that can catalyse RNA polymerization. The class I ligase ribozyme selected in vitro 15 years ago from a pool of random RNA sequences catalyses formation of a 3',5'-phosphodiester linkage analogous to a single step of RNA polymerization. Recently, the three-dimensional structure of the ligase was solved in complex with U1A RNA-binding protein and independently in complex with an antibody fragment. The RNA adopts a tripod arrangement and appears to use a two-metal ion mechanism similar to protein polymerases. Here, we discuss structural implications for engineering a true polymerase ribozyme and describe the use of the antibody framework both as a portable chaperone for crystallization of other RNAs and as a platform for exploring steps in evolution from the RNA world to the RNA-protein world.


PMID: 21930583

DOI: 10.1098/rstb.2011.0144

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