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DNA microarray gene expression profile of Mycobacterium tuberculosis when exposed to osthole

, : DNA microarray gene expression profile of Mycobacterium tuberculosis when exposed to osthole. Polish Journal of Microbiology 62(1): 23-30

Tuberculosis (TB), affecting one-third of the global population, kills an estimated two to three million people every year. The development of drug resistance is becoming a serious threat to any attempt to control this disease, which underscores the need for new agents targeting Mycobacterium tuberculosis (M. tuberculosis). Osthole (7-methoxy-8-isopentenoxycoumarin) is a coumarin derivative present in many medicinal plants. Previous studies have shown that osthole possesses antimycobacterial effects, however, the action mechanism of osthole is unclear. In the study, we used a commercial oligonucleotide microarray to determine the overall transcriptional response of M. tuberculosis H37Rv triggered by exposure to osthole. Analysis of the microarray data revealed that a total of 478 genes were differentially regulated by osthole. Of these, 241 genes were upregulated, and 237 genes were downregulated. Some of the important genes that were significantly regulated are related to different pathways such as fumarate reductase, class I peroxidase, cell wall, nitrate respiration, and protein synthesis. Real-time quantitative RT-PCR was performed for chosen genes to validate the microarray results. To our knowledge, this genome-wide transcriptomics approach has produced the first insights into the response of M. tuberculosis when exposed to osthole.


PMID: 23829074

Other references

Smithurst B.A., 1970: Treatment of acute gonococcal urethritis with 3 drug regimes in 768 males brisbane queensland 1967 69. British Journal of Venereal Diseases 46(5): 398-400

Azizbekyan, R.R., 1973: Dependence of the UV sensitivity of phage T4 on the localization of conditionally lethal (amber) mutations in the genome. Soviet Genetics 7(2): 234-238

Marschner, H.S.hropp, A., 1977: Comparative studies on the sensitivity of six rootstock varieties of grapevine to phosphate induced Zn deficiency. Vitis 16 (2) 79-88

Zhang, Y.; Cai, P.; Li, L.; Shi, L.; Chang, P.; Liang, T.; Yang, Q.; Liu, Y.; Wang, L.; Hu, L., 2016: Co-expression of TIM-3 and CEACAM1 promotes T cell exhaustion in colorectal cancer patients. T-cell immunoglobulin domain and mucin domain-3(TIM-3) is an activation induced inhibitory molecule involved in immune tolerance and is recently reported to induce T cell exhaustion which is mediated by carcinoembryonic antigen cell adhesion molec...

Filip, Z., 1967: The development of microorganisms and the production of humic substances in sand cultures with different bentonite contents. Sand (enriched with casein and treated with nutrient solution) was aerobically incubated in the presence or absence of 0.125-0.5% bentonite at 35 degrees C for 30 days. Both the development of microbial populations on nutrient and starch agar and...

Pflugfelder P.W.; Humen D.P.; O'brien P.A.; Purves P.D.; Jablonsky G.; Kostuk W.J., 1987: Comparison of bepridil with nadolol for angina pectoris. Bepridil hydrochloride is a unique calcium channel-blocking drug with anti-ischemic and type 1 antiarrhythmic properties. With a half-life of more than 40 hours, once-daily therapy is possible. Twenty-four patients (22 men, 2 women), mean age 58 y...

Wölfl, S.; Burchert, A.; Kroll, T.C., 2004: Monitoring therapy with gene expression profiling reveals physiological differences in drug action. Gene expression profiling has become a versatile tool for biomedical research, which allows the assessment of a wide variety of basic questions in cellular regulation, in particular when a large number of molecular parameters have changed. There a...

Budimirovic, D.B.; Bukelis, I.; Cox, C.; Gray, R.M.; Tierney, E.; Kaufmann, W.E., 2006: Autism spectrum disorder in Fragile X syndrome: differential contribution of adaptive socialization and social withdrawal. The present study extends our previous work on characterizing the profile of social behavior abnormalities in boys with Fragile X (FraX) and autism spectrum disorder (ASD) using clinically oriented behavioral rating scales and standardized instrum...

Shono, H.; Ohkawa, T.; Tomoda, H.; Mutai, T.; Araki, K., 2011: Fabrication of colorless organic materials exhibiting white luminescence using normal and excited-state intramolecular proton transfer processes. Organic, white luminescent materials were fabricated using a mixture of proton-transfer and nonproton-transfer fluorophores. 2'-Methoxy and 2'-hydroxy derivatives of 2-phenylimidazo[1,2-a]pyridine (PIP) have similar UV-absorption propert...

Stout, B.A.; Buchele, W.F.; Snyder, F.W., 1960: Planting for maximum seedling emergence. Maximum emergence of bean seedlings was obtained by firming the soil at seed level and covering the seeds with a 1-in. layer of loose soil, in contrast to the usual practice of placing the seeds in loose soil and pressing the surface.