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Defective eosinophil hematopoiesis ex vivo in inbred Rocky Mountain White (IRW) mice

, : Defective eosinophil hematopoiesis ex vivo in inbred Rocky Mountain White (IRW) mice. Journal of Leukocyte Biology 90(6): 1101-1109

We examine the proliferation and differentiation of bone marrow (BM) progenitors from inbred Rocky Mountain White (IRW) mice, a strain used primarily for retrovirus infection studies. In contrast to findings with BALB/c and C57BL/6 strains, IRW BM cells cannot proliferate or generate pure eosinophil cultures ex vivo in response to a defined cytokine regimen. Analysis of IRW BM at baseline was unremarkable, including 0.08 ± 0.03% Lin(-)Sca-1(+)c-kit(+) (LSK) hematopoietic stem cells and 5.2 ± 0.3% eosinophils; the percentage of eosinophil progenitors (EoPs; Lin(-)Sca-1(-)c-kit(+)CD34(+)IL-5Rα(+)) was similar in all three mouse strains. Transcripts encoding GM-CSFRα and the IL-3/IL-5/GM-CSF common β chain were detected at equivalent levels in IRW and BALB/c BM, whereas expression of transcripts encoding IL-5Rα, IL-3Rα, and GATA-2 was diminished in IRW BM compared with BALB/c. Expression of membrane-bound IL-5Rα and intracellular STAT5 proteins was also diminished in IRW BM cells. Diminished expression of transcripts encoding IL-5Rα and GATA-2 and immunoreactive STAT5 in IRW BM persisted after 4 days in culture, along with diminished expression of GATA-1. Western blot revealed that cells from IRW BM overexpress nonsignaling soluble IL-5Rα protein. Interestingly, OVA sensitization and challenge resulted in BM and airway eosinophilia in IRW mice; however, the responses were significantly blunted. These results suggest that IRW mice have diminished capacity to generate eosinophils in culture and in vivo, likely as a result of diminished signaling via IL-5Rα.


PMID: 21878543

DOI: 10.1189/jlb.0211059

Other references

Venkataraman, A.; Abraham, E.; Srinivasan, D., 1970: A note on the trial of two insecticides for the control of the red hairy caterpillar, Amsacta spp. on groundnut in Thanjavur District. Following a severe outbreak of Amsacta albistriga (Wlk.) and A. moorei (Btlr.) on groundnut in the Thanjavur district of the Indian State of Madras in October 1969, two further insecticides were tested for the control of these pests. Dichlorvos (N...

Avdiushin, S.I.; Pereiaslova, N.K.; Svidskiĭ, P.M.; Malyshev, A.B., 1983: Prediction and diagnosis of the fluxes and spectra of solar cosmic radiation. Kosmicheskaia Biologiia i Aviakosmicheskaia Meditsina 17(2): 86-89

Grippo, Jj, 1990: Taxes, records and accounting for small businesses. Conference proceedings: Income Opportunities for the Private Landowner Through Management of Natural Resources and Recreational Access edited by William N Grafton et al: 278

J.W.tterson; J.J.W.lsh; P.A.G.llespie; S.E.ston, 1996: Scaling systematics of fault sizes on a large-scale range fault map. A large-scale, high-quality fault map was produced for an intensively mined area of the U.K. South Yorkshire coalfield. This database has been used to investigate both the scaling properties of fault systems and the robustness of techniques for th...

Tenney, Sm, 1970: Respiration in mammals. Dukes H H Dukes' Physiology Of Domestic Animals Ed 8: 93-339

Lebeau, E.L.; Binstead, R.A.; Meyer, T.J., 2001: Mechanistic implications of proton transfer coupled to electron transfer. The kinetics of electron transfer for the reactions cis-[Ru(IV)(bpy)2(py)(O)]2+ + H+ + [Os(II)(bpy)3]2+ <==> cis-[Ru(III)(bpy)2(py)(OH)]2+ + [Os(III)(bpy)3]3+ and cis-[Ru(III)(bpy)2(py)(OH)]2+ + H+ + [Os(II)(bpy)3]2+ <==> cis-[Ru(II)(b...

Oya, T.; Miyazawa, M.; Tsuge, N.; Fujioka, S.; Takamiya, T., 1984: Clinical studies of leftose tablets for dental infections and their prevention. Shikai Tenbo 64(6): 1227-1237

Bartlett W.R.; Johnson W.S., 1975: The biomimetic cyclization of 1 3 di methyl 2 trans trans 7 11 dimethyl 3 7 11 dodeca trienyl 2 cyclo hexenol to give the d homo steroid nucleus 1. Bioorganic Chemistry 4(4): 342-362

Bazhanova, E.D.; Grinevich, V.V.; Danilova, O.A.; Chernigovskaya, E.V., 1998: Age-related changes in oxytocinergic neurosecretory cells in the accessory magnocellular neuroendocrine nuclei of the hypothalamus in rats. Neuroscience and Behavioral Physiology 28(4): 354-356

Frieling, F.; Hoser, N.; Steinbach, R., 1980: 25 Jahre Beobachtungsgemeinschaft Windischleubaer Stausee 56 Bericht der Beobachtungsgemeinschaft Windischleubaer Stausee. Beitraege zur Vogelkunde, 265: 245-248