+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detecting endotoxin contamination of ophthalmic viscosurgical devices: intracameral versus intravitreal assays in rabbits

, : Detecting endotoxin contamination of ophthalmic viscosurgical devices: intracameral versus intravitreal assays in rabbits. Ophthalmology 119(7): E11-E18

To compare the sensitivities of intracameral and intravitreal assays in the rabbit model to determine the relative adequacy of these methods in detecting bacterial endotoxin contamination of ophthalmic viscosurgical devices (OVDs). Experimental, randomized animal study. Twenty New Zealand white rabbits. Rabbits were randomized into 4 groups to receive a cohesive or a dispersive OVD via intracameral or intravitreal injection. All 40 treated eyes (10 eyes of 5 animals in each group) received bilateral injection of OVD spiked with bacterial endotoxin at 7.0 endotoxin units/ml. All eyes were evaluated by slit-lamp biomicroscopy for inflammatory response at 3, 6, 9, 24, 48, and 72 hours after exposure. Eyes that received intravitreal injection were also dilated at 24, 48, and 72 hours and were re-examined by slit-lamp biomicroscopy and by indirect ophthalmoscopy. Conjunctival inflammation, anterior chamber (AC) flare, cells and fibrin, vitreous haze and cells, iridal hyperemia, corneal clouding, lens opacities, and onset times. Intracamerally injected eyes frequently showed conjunctival congestion, AC cells and flare, iridal hyperemia, and fibrin within 6 hours. Up to 80% showed AC cells and flare at 9 hours, and up to 70% showed fibrin at 24 hours. These signs diminished within 48 hours. Fibrin and cells also were seen on the lens surface of most of the eyes. Intravitreally injected eyes showed no signs of inflammation within 24 hours, other than some conjunctival inflammation. After the 24-hour time point, in addition to some conjunctival inflammation, some other signs of inflammation were observed infrequently in the intravitreally injected eyes, including minor vitreous cell reaction in 2 eyes. Although there was 1 dispersive OVD-treated eye with cells and fibrin on the lens capsule at 48 hours, no aqueous cells or flare were seen in the AC of any intravitreally injected eyes at any time during the course of the study. The rabbit intravitreal assay, when limited to 72 hours, does not seem to have adequate sensitivity to detect endotoxin reliably in OVDs.


PMID: 22578451

DOI: 10.1016/j.ophtha.2012.04.005

Other references

King, J.; Dixon, R.I., 1969: A further factor contributing to inherited suxamethonium sensitivity. British Journal of Anaesthesia 41(12): 1023-1028

Ionita Manzatu, V.; Ionita Manzatu, M.; Cioca, G.; Bevacqua, A.J., 2002: Antioxidants in clusters of structured water. The invention relates to structured water and its antioxidant activity. In addition, the present invention relates to antioxidants incorporated within the cluster structure of either electropositive (S water) or electronegative (I water). The stru...

Jaśkiewicz, F.; Supeł, K.; Koniarek, Włodzimierz.; Zielińska, M., 2016: Admission hyperglycemia in patients with acute coronary syndrome complicated by cardiogenic shock. Many reports shoed that for patients with acute coronary syndrome (ACS) increased admission blood glucose (ABG) level is associated with adverse outcomes. Although scientific reports on this issue are still inconsistent, many recent studies confir...

Renberg, L.; Tarkpea, M.; Sundstrom, G., 1986: The use of the bivalve mytilus edulis as a test organism for bioconcentration studies ii. the bioconcentration of two carbon 14 labeled chlorinated paraffins. The bioconcentration of two 14C-labeled chlorinated paraffins (CP) has been studied in a flow-through test system using the common mussel Mytilus edulis. Both CPs showed a rapid uptake rate and the CP with C16 carbon chain and 34% chlorination had...

Chai, P.; Dudley, R., 1995: Maximum flight performance and limits to power output of vertebrate striated muscle. FASEB Journal 9(3): A353

Rabaud, Etienne., 1909: Sur Lina populif parasitee par Meigenia bisignata Meigen. Feuille des Jeunes Naturalistes, 39 101-102

Tsuji, N.; Honda, K.; Wada, M.; Okano, K.; Ohtake, H., 2015: Isolation and characterization of a thermotolerant ene reductase from Geobacillus sp. 30 and its heterologous expression in Rhodococcus opacus. Rhodococcus opacus B-4 cells are adhesive to and even dispersible in water-immiscible hydrocarbons owing to their highly lipophilic nature. In this study, we focused on the high operational stability of thermophilic enzymes and applied them to a b...

Cassese, S.; Byrne, R.A.; Laugwitz, K-Ludwig.; Schunkert, H.; Berger, P.B.; Kastrati, A., 2016: Bivalirudin versus heparin in patients treated with percutaneous coronary intervention: a meta-analysis of randomised trials. Current recommendations on the use of bivalirudin in patients treated with percutaneous coronary intervention (PCI) are mostly based on trials comparing bivalirudin versus heparin plus planned glycoprotein IIb/IIIa inhibitor (GPI). Whether bivalir...

Mertin, J., 1978: Multiple sclerosis and the immune system. Progress and unsolved problems. Medizinische Klinik 73(50): 1752-1767

Lisenkov, V.V.; Demidova, I.I., 1999: Experimental evaluation of teeth destruction as a result of their removal. Evaluates the causes and mechanisms of tooth fractures occurring during tooth removal, using mathematical simulation methods. Most common sites of tooth destruction during tooth removal are noted. The authors emphasize obligatory thorough examinat...