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Development of ultrabright semiconducting polymer dots for ratiometric pH sensing

, : Development of ultrabright semiconducting polymer dots for ratiometric pH sensing. Analytical Chemistry 83(4): 1448-1455

Semiconducting polymer-based nanoparticles (Pdots) have recently emerged as a new class of ultrabright probes for biological detection and imaging. This paper describes the development of poly(2,5-di(3',7'-dimethyloctyl)phenylene-1,4-ethynylene) (PPE) Pdots as a platform for designing Förster resonance energy transfer (FRET)-based ratiometric pH nanoprobes. We describe and compare three routes for coupling the pH-sensitive dye, fluorescein, to PPE Pdots, which is a pH-insensitive semiconducting polymer. This approach offers a rapid and robust sensor for pH determination using the ratiometric methodology where excitation at a single wavelength results in two emission peaks, one that is pH sensitive and the other one that is pH insensitive for use as an internal reference. The linear range for pH sensing of the fluorescein-coupled Pdots is between pH 5.0 and 8.0, which is suitable for most cellular studies. The pH-sensitive Pdots show excellent reversibility and stability in pH measurements. In this paper, we use them to measure the intracellular pH in HeLa cells following their uptake by endocytosis, thus demonstrating their utility for use in cellular and imaging experiments.


PMID: 21244093

DOI: 10.1021/ac103140x

Other references

Faredin, I.; Blaho, G., 1958: Studies of neutral 17-ketosteroids. I. Evaluation of determination of neutral 17-ketosteroids in urine and its use in clinical practice. Zeitschrift für die Gesamte Innere Medizin und Ihre Grenzgebiete 13(9-10): 339-347

Gallagher, F.; Gaubert, D.; Hale, M., 2003: Respiratory hazards of "nail sculpture". Bmj 327(7422): 1050-1050

Zalka, A.S.abo, L., 1979: Hydromotors with Orbit system on agricultural machinery. Jarmuvek mezogazdasagi gepek 26(6): 221-226

Sampson, Gp, 1987: Liberation through tariff quotas. Textile Asia 18(12): 149-155

Aubry, A.F.ancoise; Markoglou, N.; Descorps, V.; Wainer, I.W.; Felix, G., 1994: Evaluation of a chiral stationary phase based on mixed immobilized proteins. The preparation of a new chiral stationary phase (CSP) based on mixed immobilized human serum albumin (HSA) and 1-acid glycoprotein (AGP) is reported. Enantiomeric separations of basic, acidic and neutral compounds on this AGP-HSA CSP were compare...

Schatz, G.; Racker, E., 1966: Partial resolution of the enzymes catalyzing oxidative phosphorylation. VII. Oxidative phosphorylation in the diphosphopyridine nucleotide-cytochrome b segment of the respiratory chain: assay and properties in submitochondrial particles. Journal of Biological Chemistry 241(6): 1429-1438

D.Bruyne, J.I., 1948: Experiences with penicillin treatment of congenital syphilis. 10 infants,2-4 mos. old, suffering from congenital syphilis, were given penicillin, 150,000 U./kg. body wt., in 11-14 days. In 8 of the patients the result was favorable; 2 died, one with a severe reaction following the 1st injn. of penicillin, th...

Pompili, M.; Baldessarini, R.J.; Berman, A.L.; Lester, D.; Wasserman, D.; D.L.o, D.; Girardi, P., 2012: Integration of suicidology with general medicine: an obligation to society. Patient Education and Counseling 85(1): 127-128

Raithel D.; Kasprzak P.M.; Ritter W.; Zeitler E., 1991: Indication and results of surgical therapy and ptrd in renal artery stenoses. Journal of Cardiovascular Surgery 32(4 SUPPL): 81

Gardner C.R.; Colton C.K.; Langer R.S.; Hamilton B.K.; Archer M.C.; Whitesides G.M., 1974: Enzymatic regeneration of atp from amp and adp part 1 thermodynamics kinetics and process development. Pye, E Kendall And Lemuel B Wingard, Jr (Ed ) Enzyme Engineering, Vol 2 Conference, Henniker, N H , U S A , Aug 5-10, 1973 Xiv+470p Illus Plenum Press: New York, N Y , U S A ; London, England Isbn 0-306-35282-6 209-216