+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Differential expression of mTOR signalling components in drug resistance in ovarian cancer

, : Differential expression of mTOR signalling components in drug resistance in ovarian cancer. Anticancer Research 30(9): 3529-3534

A limitation to successful cancer chemotherapy treatments is the acquisition of drug resistance. In advanced-stage ovarian cancer, the mammalian target of rapamycin (mTOR) pathway is up-regulated, and inhibition of this pathway increases chemosensitivity in ovarian carcinoma cell lines. In this study, the expression of DEPTOR, mTOR, RICTOR, RAPTOR and S6 kinases were investigated in SKOV-3 and PEO1 parental and the paclitaxel-resistant (TaxR) SKOV-3TaxR and PEO1TaxR cell lines. RT-PCR, immunofluorescent analysis and Western blotting were carried out. Quantitative RT-PCR revealed significant up-regulation of DEPTOR in both paclitaxel-resistant cell lines. SKOV-3TaxR exhibited down-regulation of RICTOR, RAPTOR and mTOR, whereas PEO1-TaxR showed down-regulation of RAPTOR and up-regulation of RICTOR and mTOR. Semi-quantitative RT-PCR analysis revealed marked changes in the expression of p70S6K splice variants mRNA in PEO1TaxR. Moreover, the phosphorylation status of p70S6K at Ser371 appears to be cell-type specific. We hypothesize that mTOR signalling may play a role in mediating paclitaxel resistance in ovarian cancer.


PMID: 20944133

Other references

Anonymous, 1933: Orange rust of Blackberry and Raspberry. A popular note is given on the symptoms, life-history, and control of the orange rust of blackberry and raspberry (Gymnoconia interstitialis) in New Jersey.

Högl, B., 2017: Circadian Rhythms and Chronotherapeutics-Underappreciated Approach to Improving Sleep and Wakefulness in Parkinson Disease. JAMA Neurology 74(4): 387-388

Singsit, S., 2003: Update on Manipur brow-antlered deer or sangai (Cervus eldi eldi) in Leibul Lamjao National Park in Manipur. Zoos' Print Journal. December; 1812: 7-9

Murayama, J., 1935: Des especes des Platypides du domaine de l'Empire de Japon ainsi que la dovoration par ces insectes. J For Sci Soc Tokyo, 1929: 669-682

Kwok, O-Hing.; Prpic, R.; Gaspar, J.; Mathey, D.G.; Escobar, A.; Goldar-Najafi, A.; Reifart, N.; Ischinger, T.A.; Popma, J.J., 2002: Angiographic outcome after intracoronary X-Sizer helical atherectomy and thrombectomy: first use in humans. The objective of this study was to evaluate the early angiographic outcome in the first human subjects who underwent intracoronary atherectomy and thrombectomy using the X-Sizer helical cutting and aspiration system. Percutaneous coronary interven...

Wen-Hsien Hsu; Ting-Chen Chang; Teng-Chin Tsai; Chin-Long Lee; Davina Rene Hsu, 2012: Managing May–Thurner Syndrome A Review Focusing on Evolving Treatment Modalities. May Thurner syndrome (MTS), also known as iliac vein compression syndrome, was first reported in 1957 by May and Thurner on the basis of dissecting 430 cadavers. MTS is a clinical entity of left iliac vein compression by the anteriorly positioned...

Fajardo, D.A.; Cheung, J.; Ito, C.; Sugawara, E.; Nikaido, H.; Misra, R., 1998: Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels. A novel porin, OmpG, is produced in response to a chromosomal mutation termed cog-192. Molecular characterization of cog-192 revealed that it is a large chromosomal deletion extending from the 3' end of pspA through to the 5' end of an o...

Robson, E., 2007: So you want to be a midwife? Part 5: Preparing for the interview. Practising Midwife 10(10): 49-49

Pinho-Guedes, N.; Maria, J.Z.mbolim, L.V.ntura, J., 1997: Protoplast isolation of Ananas comosus (L.) Merr. cv. Perolera. Acta Horticulturae 425: 259-266

Proven, A.L.; Rowlands, A., 1944: Testing pasteurized milk. Bacteriological examination of pasteurized milk is primarily to determine recontamination after pasteurization and to estimate keeping quality in the hands of the consumer. Therefore, simple tests other than colony and coliform counts should be ca...