geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Diffuse decapping enzyme DCP2 accumulates in DCP1 foci under heat stress in Arabidopsis thaliana


, : Diffuse decapping enzyme DCP2 accumulates in DCP1 foci under heat stress in Arabidopsis thaliana. Plant & Cell Physiology 56(1): 107-115

The decapping enzymes DCP1 and DCP2 are components of a decapping complex that degrades mRNAs. DCP2 is the catalytic core and DCP1 is an auxiliary subunit. It has been assumed that DCP1 and DCP2 are consistently co-localized in cytoplasmic RNA granules called processing bodies (P-bodies). However, it has not been confirmed whether DCP1 and DCP2 co-localize in Arabidopsis thaliana. In this study, we generated DCP1-green fluorescent protein (GFP) and DCP2-GFP transgenic plants that complemented dcp1 and dcp2 mutants, respectively, to see whether localization of DCP2 is identical to that of DCP1. DCP2 was present throughout the cytoplasm, whereas DCP1 formed P-body-like foci. Use of DCP1-GFP/DCP2-red fluorescent protein (RFP) or DCP1-RFP/DCP2-GFP plants showed that heat treatment induced DCP2 assembly into DCP1 foci. In contrast, cold treatment did not induce DCP2 assembly, while the number of DCP1 foci increased. These changes in DCP1 and DCP2 localization during heat and cold treatments occurred without changes in DCP1 and DCP2 protein abundance. Our results show that DCP1 and DCP2 respond differently to environmental changes, indicating that P-bodies have diverse DCP1 and DCP2 proportions depending on environmental conditions. The localization changes of DCP1 and DCP2 may explain how specific mRNAs are degraded during changes in environmental conditions.

US$19.90

PMID: 25339350

DOI: 10.1093/pcp/pcu151


Other references

Guna, H.Q.an; Takeo, K.; Yarita, K.; Yoshida, S.; Nishimura, K.; Miyaji, M., 1995: Rapid and extensive vacuolation of the budding yeasts Saccharomyces cerevisiae and Candida albicans by amphotericin B. The effect of amphotericin B (AMPH) on vacuolation in the budding yeasts Saccharomyces cerevisiae and Candida albicans was studied. The minimum inhibitory concentration of AMPH for growth of S. cerevisiae and C. albicans was 1 mu-g/ml. In untreate...

Garvy, B.A.; Gigliotti, F.; Harmsen, A.G., 1997: Neutralization of interferon-gamma exacerbates pneumocystis-driven interstitial pneumonitis after bone marrow transplantation in mice. The role of IFN-gamma in the development of infection-driven interstitial pneumonitis in a model of murine graft-versus-host disease was investigated. Mice were given either syngeneic or allogeneic bone marrow transplants along with lung Pneumocys...

VA.Someren, E.J.W., 2007: Improving actigraphic sleep estimates in insomnia and dementia: how many nights?. In order to investigate how the duration of actigraphic recordings affects the reliability of actigraphic estimates of sleep and 24-h activity rhythm variables, two to 3 weeks of actigraphy were recorded, from which pairs of variables derived fro...

Tew J.G.; Brumeister J.A.; Palcanis K.G.; Ranney R.R., 1983: Spontaneous lymphocyte proliferation and the periodontal status of young adults. Whether the level of incorporation of 3H-thymidine in unstimulated cultures of peripheral blood lymphocytes obtained from young adults differs as a function of their periodontal status was studied. Kinetic studies using normal PBL [peripheral bloo...

Feldman, C.; Anderson, R.; Kanthakumar, K.; Vargas, A.; Cole, P.J.; Wilson, R., 1994: Oxidant-mediated ciliary dysfunction in human respiratory epithelium. Exposure of human nasal ciliated epithelium to reactive oxidants generated by the enzymatic xanthine-xanthine oxidase superoxide/hydrogen peroxide (H-2O-2) and glucose-glucose oxidase H-2O-2-generating systems, or to reagent H-2O-2 or hypochlorous...

Rodrigues, J.Olsen.; Galbiatti, A.Lívia.Silva.; Ruiz, M.Torreglosa.; Raposo, L.Sergio.; Maniglia, Jé.Victor.; Pavarino-Bertelli, E.Cristina.; Goloni-Bertollo, E.Maria., 2011: Polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene and risk of head and neck squamous cell carcinoma. Methylenetetrahydrofolate reductase gene (MTHFR) C677T polymorphism may be a risk factor for head and neck squamous cell carcinoma due to changes in folate levels that can induce disorders in the methylation pathway, which results in carcinogenesi...

Goodwin, W.E.; Shumacker, H.B., 1947: Aneurysm of the hypogastric artery producing urinary tract obstruction; report of a case. Journal of Urology 57(5): 839-844

Petzel, S.V.; Solso, R.L., 1978: Response attributes and anxiety drive in paired associate learning. The effect of generalized drive (measured by the Taylor Manifest Anxiety Scale), meaningfulness and similarity was examined in a paired associate learning tasks with human subjects. Learning was analyzed in 2 stages, a response-learning (RL) and a...

Peeters, R.; Hubens, A., 1988: The mesh skin graft--true expansion rate. Using the mesh graft II dermatome (Zimmer) for split skin graft expansion, the 3 to 1 and 1.5 to 1 expansion rates were evaluated for true clinical expansion. In one hundred and one 1.5 to 1 expanded grafts the actual expansion was 1.2 and in sixt...

Lamprecht, M.P.; Merwe, W.J.C. van der, 1976: Effect of spacing and topping of plants on yield and quality of Oriental tobacco. In trials in 3 growing season at Elsenburg, tobacco cv. Soulouk was planted at 10, 15 or 20 cm apart within rows 75 cm apart and was topped to give (a) 1 333 300, (b) 1 599 960 or (c) 1 866 620 leaves/ha or (d) was not topped (28 leaves/plant) in...