+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Disposition of phenolic and sulfated metabolites after inhalation exposure to 4-chlorobiphenyl (PCB3) in female rats

, : Disposition of phenolic and sulfated metabolites after inhalation exposure to 4-chlorobiphenyl (PCB3) in female rats. Chemical Research in Toxicology 27(8): 1411-1420

PCBs, such as PCB3, are air contaminants in buildings and outdoors. Metabolites of PCB3 are potential endocrine disrupting chemicals and genotoxic agents. We studied the disposition of phenolic and sulfated metabolites after acute nose-only inhalation exposure to airborne PCB3 for 2 h in female rats. Inhalation exposure was carried out in three groups. In the first group, rats exposed to an estimated dose of 26 μg/rat were euthanized at 0, 1, 2, and 4 h after exposure. Highest concentrations of phenols and sulfates were observed at 0 h, and the values were 7 ± 1 and 560 ± 60 ng/mL in serum, 213 ± 120 and 842 ± 80 ng/g in liver, 31 ± 27 and 22 ± 7 ng/g in lung, and 27 ± 6 and 3 ± 0 ng/g in brain, respectively. First-order serum clearance half-lives of 0.5 h for phenols and 1 h for sulfates were estimated. In the second group, rats exposed to an estimated dose of 35 μg/rat were transferred to metabolism cages immediately after exposure for the collection of urine and feces over 24 h. Approximately 45 ± 5% of the dose was recovered from urine and consisted mostly of sulfates; the 18 ± 5% of the dose recovered from feces was exclusively phenols. Unchanged PCB3 was detected in both urine and feces but accounted for only 5 ± 3% of the dose. Peak excretion of metabolites in both urine and feces occurred within 18 h postexposure. In the third group, three bile-cannulated rats exposed to an estimated dose of 277 μg/rat were used for bile collection. Bile was collected for 4 h immediately after 2 h exposure. Biliary metabolites consisted mostly of sulfates, some glucuronides, and lower amounts of the free phenols. Control rats in each group were exposed to clean air. Clinical serum chemistry values, serum T4 level, and urinary 8-hydroxy-2'-deoxyguanosine were similar in treated and control rats. These data show that PCB3 is rapidly metabolized to phenols and conjugated to sulfates after inhalation and that both of these metabolites are distributed to liver, lungs, and brain. The sulfates elaborated into bile are either reabsorbed or hydrolyzed in the intestine and excreted in the feces as phenols.


PMID: 24988477

DOI: 10.1021/tx500150h

Other references

Munakata, T.; Adachi, N.; Yokoyama, N.; Kuzuhara, T.; Horikoshi, M., 2000: A human homologue of yeast anti-silencing factor has histone chaperone activity. Background: Structural changes in chromatin play essential roles in regulating eukaryotic gene expression. Silencing, potent repression of transcription in Saccharomyces cerevisiae, occurs near telomeres and at the silent mating-type loci, as well...

McAfee, J.G.; Gagne, G.; Atkins, H.L.; Kirchner, P.T.; Reba, R.C.; Blaufox, M.D.; Smith, E.M., 1979: Biological distribution and excretion of DTPA labeled with Tc-99m and In-111. For the purpose of radiation dose estimates, organ assays and excretion measurements of the Tc-99m and In-111 complexes with DTPA were conducted in dogs at various time intervals up to 24 hr, and the results compared with available human data. The...

Habbe, K.; Pfortner, W., 1951: Cholinesterase-serum influenced by sex hormones; on the problem of neurohormonal regulation. Deutsche Medizinische Wochenschrift 76(9): 269-272

Sardar A.K.; Mukherjee K.K., 1987: Interspecific hybridization in trichosanthes anguina l. Two species of Trichosanthes, the cultivated T. anguina and wild T. cucumerina, were hybridized. The two parents and their F1 hybrids were investigated for leaf, flower, fruit and seed characters. Chiasma frequency, leaf phenolics, oil content and...

Wyrsch, J., 1964: On Sexual Psychopathology And Its General Significance. Schweizer Archiv für Neurologie, Neurochirurgie und Psychiatrie 94: 185-201

Anonymous, 1991: JCAHO standards changes for 1992. Hospital Food & Nutrition Focus 8(2): 1, 3-1, 5

Puthz, V., 1974: A new Stenus species from the Iberian peninsula (Coleoptera, Staphylinidae). 137th contribution to the knowledge of Steninae. Revue suisse de zoologie: 81 (1) 301-303

Schramm, H.; Weber, P.M.; Götze, M.; Clausner, G., 1980: Surgical intervention in cases of gastro-duodenal ulcer bleeding. Results in the treatment of the bleeding gastro-duodenal ulcer during a period of 21 years are analysed. The results refer to an early surgery intervention. A bleeding-stop must be tried, particularly in the elderly patient. If possible a causal t...

Zimmermann, E.; Zimmermann, P.; Zimmermann, A., 1980: Vergleich einiger Kommunikationsformen funf nonhumaner Primatenarten. Zeitschrift des Koelner Zoo, 231: 3-20

Gross, R.; Bertrand, G.; Ribes, G.; Loubatières-Mariani, M.M., 1987: Alpha 2-adrenergic potentiation of adenosine-stimulating effect on glucagon secretion. Previous studies from our laboratory showed 1) that adenosine (1.65 microM), a substance released by tissues in energy-deficient states, stimulated glucagon secretion by activation of A2 purinergic receptors, and 2) that this effect was potentiate...