geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Dynamic and high-resolution live cell imaging by direct electron beam excitation


, : Dynamic and high-resolution live cell imaging by direct electron beam excitation. Optics Express 20(5): 5629-5635

We propose a direct electron-beam excitation assisted optical microscope with a resolution of a few tens of nanometers and it can be applied for observation of dynamic movements of nanoparticles in liquid. The technique is also useful for live cell imaging under physiological conditions as well as observation of colloidal solution, microcrystal growth in solutions, etc. In the microscope, fluorescent materials are directly excited with a focused electron beam. The direct excitation with an electron beam yields high spatial resolution since the electron beam can be focused to a few tens of nanometers in the specimens. In order to demonstrate the potential of our proposed microscope, we observed the movements of fluorescent nanoparticles, which can be used for labelling specimens, in a water-based solution. We also demonstrated an observation result of living CHO cells.

US$29.90

PMID: 22418370


Other references

Voelckel, C.; Baldwin, I.T., 2004: Generalist and specialist lepidopteran larvae elicit different transcriptional responses in Nicotiana attenuata, which correlate with larval FAC profiles. Unlike generalist herbivores, specialists are believed to share a history of evolutionary interactions with their host plants. We determine whether a specialist lepidopteran species alters plant metabolism differently from two generalist species d...

Botter, G.; Daly, E.; Porporato, A.; Rodriguez Iturbe, I.; Rinaldo, A., 2008: Probabilistic dynamics of soil nitrate: coupling of ecohydrological and biogeochemical processes. In this paper we analyze the probabilistic dynamics resulting from a simplified model of soil moisture and nitrate mass in relatively arid environments, which accounts for relevant hydrologic and biogeochemical processes and for the random charact...

Franck, I., 1983: Understanding Jewish biomedical ethics: reflections on the papers. Franck, editor of a journal issue containing seven articles on Jewish biomedical ethics, categorizes the articles as illustrating different modes of explication of the encounter between Jewish and general biomedical ethics. He describes Immanuel J...

Bellometti, S.; Richelmi, P.; Tassoni, T.; Bertè, F., 2005: Production of matrix metalloproteinases and their inhibitors in osteoarthritic patients undergoing mud bath therapy. Several studies have demonstrated that matrix metalloproteinases (MMPs) are frequently implicated in the destruction of articular cartilage in arthritis. The control of MMP activity is dependent on the local concentration of tissue inhibitors of m...

Dan, D.; Collure, D.W.; Hoover, E.L., 2003: Bouveret's syndrome: revisiting gallstone obstruction of the duodenum. Bouveret's Syndrome is obstruction of the duodenum secondary to an impacted gallstone, usually without the presence of pneumobilia. With the steadily increasing life expectancy, greater numbers of these cases are being seen. Gallstones enter...

Chang, F.Y.; Chen, C.F.; Chen, T.C.; Fong, M.S., 1982: Primary small intestinal malignancies. The frequency of malign tumors in the small intestine was 1% of all malign tumors of the digestive tract. 4% of malign tumors found by autopsy in the digestive tract were located in the small intestine. 12 months passed between first symptoms and...

Czerny J., 1992: Hydrothermal mineralization phenomena in Karniowice travertine near Cracow. Mineralogia Polonica 23(2): 3-13

Hoffmann, M.; Zhao, S.; Luo, Y.; Li, C.; Folster, J.P.; Whichard, J.; Allard, M.W.; Brown, E.W.; McDermott, P.F., 2012: Genome sequences of five Salmonella enterica serovar Heidelberg isolates associated with a 2011 multistate outbreak in the United States. Salmonella enterica serovar Heidelberg has caused numerous outbreaks in humans. Here, we report draft genomes of five isolates of serovar Heidelberg associated with the recent (2011) multistate outbreak linked to ground turkey in the United States...

Strumeyer, C.; Mazière, M., 2006: Breast feeding or holding an infant against oneself. Soins. Pediatrie, Puericulture: 33-34

Yasuhara Hideaki; Polak Amir; Mitani Yasuhiro; Grader Abraham S.; Halleck Phillip M.; Elsworth Derek, 2006: Evolution of fracture permeability through fluid-rock reaction under hydrothermal conditions. We report flow-through experiments on a natural fracture in novaculite under moderate effective stresses ( approximately 1.4 MPa) and temperatures (20-120 degrees C) to examine the effect on flow and transport characteristics. The efflux of fluid...