geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

ECM protein nanofibers and nanostructures engineered using surface-initiated assembly


, : ECM protein nanofibers and nanostructures engineered using surface-initiated assembly. Journal of Visualized Experiments 86): -

The extracellular matrix (ECM) in tissues is synthesized and assembled by cells to form a 3D fibrillar, protein network with tightly regulated fiber diameter, composition and organization. In addition to providing structural support, the physical and chemical properties of the ECM play an important role in multiple cellular processes including adhesion, differentiation, and apoptosis. In vivo, the ECM is assembled by exposing cryptic self-assembly (fibrillogenesis) sites within proteins. This process varies for different proteins, but fibronectin (FN) fibrillogenesis is well-characterized and serves as a model system for cell-mediated ECM assembly. Specifically, cells use integrin receptors on the cell membrane to bind FN dimers and actomyosin-generated contractile forces to unfold and expose binding sites for assembly into insoluble fibers. This receptor-mediated process enables cells to assemble and organize the ECM from the cellular to tissue scales. Here, we present a method termed surface-initiated assembly (SIA), which recapitulates cell-mediated matrix assembly using protein-surface interactions to unfold ECM proteins and assemble them into insoluble fibers. First, ECM proteins are adsorbed onto a hydrophobic polydimethylsiloxane (PDMS) surface where they partially denature (unfold) and expose cryptic binding domains. The unfolded proteins are then transferred in well-defined micro- and nanopatterns through microcontact printing onto a thermally responsive poly(N-isopropylacrylamide) (PIPAAm) surface. Thermally-triggered dissolution of the PIPAAm leads to final assembly and release of insoluble ECM protein nanofibers and nanostructures with well-defined geometries. Complex architectures are possible by engineering defined patterns on the PDMS stamps used for microcontact printing. In addition to FN, the SIA process can be used with laminin, fibrinogen and collagens type I and IV to create multi-component ECM nanostructures. Thus, SIA can be used to engineer ECM protein-based materials with precise control over the protein composition, fiber geometry and scaffold architecture in order to recapitulate the structure and composition of the ECM in vivo.

US$19.90

PMID: 24799041

DOI: 10.3791/51176


Other references

Hoebee, B.; D.S.oppelaar, J.M., 1996: The isolation of rat chromosome probes and their application in cytogenetic tests. This paper reviews the rat chromosome probes which have so far been isolated in our laboratory. The probes can be divided in three groups: a centromere specific probe. chromosome-specific point probes and chromosome-specific paint probes. The cent...

Manna, R.; Ricci, V.; Curigliano, V.; Pomponi, M.; Adamo, F.; Costa, A.; D.S.cio, G.; Garbarrini, G.; D'Onofrio, F.; Bria, P., 2006: Psychiatric manifestations as a primary symptom in antiphospholipid syndrome. Antiphospholipid syndrome is a disorder characterised by recurrent venous or arterial thrombosis and/or foetal losses associated with typical laboratory abnormalities. The initial manifestation of anthiphospholipid syndrome can involve many organ...

Dolle, V.; Fan, E.; Nguyen, C.H.ng; Aubertin, A.M.rie; Kirn, A.; Andreola, M.L.ne; Jamieson, G.; Tarrago Litvak, L.; Bisagni, E., 1995: A new series of pyridinone derivatives as potent non-nucleoside human immunodeficiency virus type 1 specific reverse transcriptase inhibitors. 4-(Arylthio)-pyridin-2(1H)-ones variously substituted in their 3-, 5-, and 6-positions have been synthesized as a new series of 1-((2-hydroxyethoxy)methyl)-6-(phenylthio)thymine (HEPT)pyridinone hybrid molecules. Biological studies revealed that s...

Filler R., 1976: American chemical society symposium series 28 biochemistry involving carbon fluorine bonds chicago illinois usa august 26 1975. The topics discussed in this volume include: fluorocarboxylic acids as enzymatic and metabolic probes; the effect of aliphatic fluorine on amine drugs; organic fluorocompounds in human plasma; cis-trans perfluorodecalin emulsions; brominated fluor...

Fowler, J., 2015: Written communication: from staff nurse to nurse consultant. Part 11: CVs and letters of application. British Journal of Nursing 24(3): 190-190

Buxton, L., 1988: Unit Trusts or Pure Endowment policies--which will serve your needs best?. Nursing Rsa 3(6): 27-27

Brameld, K.; Holman, D'Arcy.; Moorin, R., 2006: Possession of health insurance in Australia--how does it affect hospital use and outcomes?. To assess the effect of possession of private health insurance on hospital use and outcomes in Western Australia. Hospital morbidity records were extracted from the Western Australian (WA) Data Linkage System for all 22 major diagnostic categories...

Genazzani, A.R.; Pluchino, N., 2016: DHEA replacement for postmenopausal women: have we been looking in the right direction?. Dehydroepiandrosterone (DHEA) and its sulfate represent the most abundant sex steroid in humans. In addition to age-related reduction, serum DHEA shows large interindividual variability. Although cross-sectional studies suggest that lower levels a...

Webster, K.A.; Discher, D.J.; Sato, B.; Bodi, I.; Bishopric, N.H., 1993: Induction of immediate-early genes and hypertrophic marker transcripts by hypoxia-reoxygenation cycles in rodent cardiac myocytes. Journal of Cellular Biochemistry Supplement 0(17 PART D): 237

Watari J.; Takata Y.; Ogawa M.; Murakami J.; Koshino S., 1991: Breeding of flocculent industrial saccharomyces cerevisiae strains by introducing the flocculation gene flo1. We have cloned a DNA fragment bearing the putative FLO1 gene that controls flocculence in Saccharomyces cerevisiae and showed that the cloned gene is on chromosome I. The flocculation property conferred by the cloned gene in a YCp-type plasmoid (s...