+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Eccentric loading of fluorogen with aggregation-induced emission in PLGA matrix increases nanoparticle fluorescence quantum yield for targeted cellular imaging

, : Eccentric loading of fluorogen with aggregation-induced emission in PLGA matrix increases nanoparticle fluorescence quantum yield for targeted cellular imaging. Small 9(11): 2012-2019

A simple strategy is developed to prepare eccentrically or homogeneously loaded nanoparticles (NPs) using poly (DL-lactide-co-glycolide) (PLGA) as the encapsulation matrix in the presence of different amounts of polyvinyl alcohol (PVA) as the emulsifier. Using 2,3-bis(4-(phenyl(4-(1,2,2-triphenylvinyl)-phenyl)amino)-phenyl)-fumaronitrile (TPETPAFN), a fluorogen with aggregation-induced emission (AIE) characteristics, as an example, the eccentrically loaded PLGA NPs show increased fluorescence quantum yields (QYs) as compared to the homogeneously loaded ones. Field emission transmission electron microscopy and fluorescence lifetime measurements reveal that the higher QY of the eccentrically loaded NPs is due to the more compact aggregation of AIE fluorogens that restricts intramolecular rotations of phenyl rings, which is able to more effectively block the non-radiative decay pathways. The eccentrically loaded NPs show far red/near infrared emission with a high fluorescence QY of 34% in aqueous media. In addition, by using poly([lactide-co-glycolide]-b-folate [ethylene glycol]) (PLGA-PEG-folate) as the co-encapsulation matrix, the obtained NPs are born with surface folic acid groups, which are successfully applied for targeted cellular imaging with good photostability and low cytotoxicity. Moreover, the developed strategy is also demonstrated for inorganic-component eccentrically or homogeneously loaded PLGA NPs, which facilitates the synthesis of polymer NPs with controlled internal architectures.


PMID: 23404950

DOI: 10.1002/smll.201202505

Other references

Pascolini, R., 1966: Ricerche sulla rigenerazione del faringe in Planaria. Bollettino della Societa Italiana di Biologia Sperimentale, 42: 968-969

Friedrich, F.J.; Glenn, C.G.; Marin, O.S., 1984: Interruption of phonological coding in conduction aphasia. A case study of conduction aphasia, investigating single word repetition, phonological coding, and short-term memory, is reported. Evidence from intact adults suggests that repetition can occur through either a lexical route or a direct auditory-a...

Zhang, S.; Finkelstein, A.; Ballard, J.; Tweten, R.; Collier,, 2003: Mapping the lumen-facing amino acids of anthrax protective antigen. Biophysical Journal 84(2 Part 2): 174a, February

Pellagatti, A.; Dolatshad, H.; Yip, B.Ham.; Valletta, S.; Boultwood, J., 2015: Application of genome editing technologies to the study and treatment of hematological disease. Genome editing technologies have advanced significantly over the past few years, providing a fast and effective tool to precisely manipulate the genome at specific locations. The three commonly used genome editing technologies are Zinc Finger Nucl...

Ito, K.; Kameyama, T.; Morinaga, F.; Takenaka, M.; Sujaku, C., 1976: Granular cell tumor of the gingiva of a newborn: report of a case. Nihon Koku Geka Gakkai Zasshi 22(4): 506-512

Pishkin, V.; Mackenthun, D.H.; Stump, B.E., 1961: Experimental attitudes affecting behavioral changes in neuropsychiatric patients. American Journal of Occupational Therapy 15: 57-60

Vedrinne, J.M.; Desjoyaux, E.; Fromage, P.; Gayet, C.; Andre-Fouet, X.; Milon, H., 1988: Bilateral apical pulmonary edema: an unusual manifestation of acute mitral insufficiency. Presse Medicale 17(19): 964-964

Stapleton, R.P., 1970: Ultrastructure of tests of some recent benthic hyaline foraminifera. Diss: 3593.

Payne S.F.; Lemp G.F.; Franks D.F.; Rutherford G.W., 1990: Survival following diagnosis of aids related opportunistic infections in san francisco california usa. Sixth International Conference on Aids Sixth International Conference on Aids, Vols 1-3 Pagination Varies Sixth International Conference on Aids University Of California San Francisco: San Francisco, California, Usa Illus Maps Paper : abstract Thc693

Aufderklamm, S.; Todenhöfer, T.; Hennenlotter, Jörg.; Gakis, G.; Mischinger, J.; Mundhenk, J.; Germann, M.; Stenzl, A.; Schwentner, C., 2014: Bilateral laparoscopic postchemotherapy retroperitoneal lymph-node dissection in nonseminomatous germ cell tumors--a comparison to template dissection. Retroperitoneal lymph node dissection (RPLND) is performed in patients with advanced nonseminomatous (NSGCT) germ cell tumors and residual retroperitoneal mass post-chemotherapy. The extent of node dissection remains unclear. Ipsilateral template...