geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of blocking endogenous miR-23a on the proliferation and invasion in gastric adenocarcinoma cell line MGC803


, : Effect of blocking endogenous miR-23a on the proliferation and invasion in gastric adenocarcinoma cell line MGC803. Nan Fang Yi Ke Da Xue Xue Bao 33(5): 678-683

To investigate the effect of functional blocking of endogenous miR-23a with a specific antisense oligonucleotide (ASO) on the proliferation and invasiveness of gastric adenocarcinoma cell line MGC803 in vitro. A specific ASO targeting miR-23a, namely ASO-23a, was transfected into MGC803 cells to block endogenous miR-23a. The mRNA level of miR-23a in the transfected cells was detected with quantitative real-time PCR. The changes of cell proliferation following the transfection were detected with MTT assay and colony formation assay, and TUNEL assay and Transwell assay were employed to evaluate the changes in cell apoptosis and invasiveness, respectively. Quantitative real-time PCR demonstrated efficient functional blocking of endogenous miR-23a in MGC803 cells by ASO-23a. Suppression of miR-23a with ASO-23a obviously inhibited cell growth, colony formation and invasiveness of MGC803 cells and significantly enhanced the cell apoptosis. ASO-23a can efficiently block the function of endogenous miR-23a in MGC803 cells to inhibit cell proliferation and invasion and promote cell apoptosis.

US$29.90

PMID: 23688986


Other references

Kostka, J.; Kostka, T.; Borowiak, E., 2016: Physical Activity in Older Adults in Relation to Place of Residence and Coexistent Chronic Diseases. The goal of this study was to assess the physical activity (PA) and its determinants of older people living in the three different environments. Three equal (n=693 each) groups of individuals aged ≥65 years living in urban, rural and institution...

Wells, C.J.L., 1951: The riddle of general practice. Postgraduate Medical Journal 27(307): 237-242

Klein, S.A., 2002: Measuring, estimating, and understanding the psychometric function: a commentary. The psychometric function, relating the subject's response to the physical stimulus, is fundamental to psychophysics. This paper examines various psychometric function topics, many inspired by this special symposium issue of Perception &...

Alavi M.; Moore S., 1982: Effect of hyper cholesterolemia on low density lipo protein uptake by neointima of rabbits. Federation Proceedings 41(3): ABSTRACT 308

Wingler, A.L.a, P.; Leegood, R., 1997: Control of photosynthesis in barley plants with reduced activities of glycine decarboxylase. A mutant (LaPr 87/30) of barley (Hordeum vulgare L.) deficient in glycine decarboxylase (GDC; EC 2.1.2.10) was crossed with wild-type plants to generate heterozygous plants with reduced GDC activities. Plants of the F2 generation were grown in air...

Maier, R.S.; Stein, D.L., 2001: Noise-activated escape from a sloshing potential well. We treat the noise-activated escape from a one-dimensional potential well of an overdamped particle, to which a periodic force of fixed frequency is applied. Near the well top, the relevant length scales and the boundary layer structure are determ...

Skarda, J.; Hemrova Sedinova, V.; Mikulas, I.; Urbanova, E.; Skardova, O., 1989: Methods of determining the number of somatic cells in cow milk. Different methods of determining the number of somatic cells in cow milk, including direct filter microscopy, DNA filter method, Coulter Counter (ECC) and Fossomatic (FOSS) apparatus, and the California mastitis test provided different results. Th...

Holwager, D., 2015: The Community Dental Health Coordinator: beyond the pilot study. Journal 94(3): 14-15

Ko, J.; Shin, S.Min.; Oh, Y.Mi.; Lee, Y.Soo.; Ryoo, Z.Yoong.; Lee, Y.Han.; Na, D.Sun.; Kim, J.Woo., 2003: Transgenic mouse model for breast cancer: induction of breast cancer in novel oncogene HCCR-2 transgenic mice. Transgenic mice containing novel oncogene HCCR-2 were generated to analyse the phenotype and to characterize the role of HCCR-2 in cellular events. Mice transgenic for HCCR-2 developed breast cancers and metastasis. The level of p53 in HCCR-2 tran...

Dukes, I.D.; Vaughan Williams, E.M.; Dennis, P.D., 1986: Electrophysiological and cardiovascular effects of pirmenol, a new class 1 antiarrhythmic drug. Pirmenol, a new antiarrhythmic agent, has been studied in the pithed rat and in the sinoatrial (SA) node, atrium, atrioventricular (AV) node, Purkinje cells, and ventricular muscle of the isolated rabbit heart. It resembles disopyramide chemically...