+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of beta-catenin in HT-29 cells

, : Effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of beta-catenin in HT-29 cells. Zhong Nan Da Xue Xue Bao. Yi Xue Ban 35(2): 140-145

To explore the effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of beta-catenin in human colorectal cancer cells HT-29. The recombinant plasmids were transfected into HT-29 cells mediated by lipofectamine(TM) 2000, and detected by green fluorescence and RT-PCR. Western blot was applied to detect beta-catenin expression level in HT-29 cells after transfection, and gray scales of electrophoresis strips were analyzed by SPSS 13.0. Green fluorescence and RT-PCR made clear that all 5 recombinant plasmids were successfully expressed in HT-29 cells. Western blot showed that beta-catenin expression level in HT-29 cells was not affected after being transfected with pEGFP-N3-APC1, pEGFP-N3-APC2 and pEGFP-N3-APC3, and was distinctly affected after being transfected with pEGFP-N3-APC4 and pEGFP-N3-APC5, especially the later one. The selected APC5 gene fragment with 15-amino acid repeats and SAMP repeats, which is relatively short, can degrade beta-catenin level in HT-29 cells and may be applied in the gene therapy.


PMID: 20197613

DOI: 10.3969/j.issn.1672-7347.2010.02.009

Other references

Webster S.; Travis M.; Webster C.; Rich K.; Silberstein L.; Blau H.M., 1988: Spatial and temporal regulation of myosin heavy chain isoform expression during human skeletal muscle development. Journal of Cellular Biochemistry Supplement (12 PART C): 345

Lazar, M.A.; Mefford, I.N.; Barchas, J.D., 1982: Tyrosine hydroxylase ec activation comparison of in vitro phosphorylation and in vivo administration of haloperidol. Rat striatal tyrosine hydroxylase (TH) was assayed 2 h after treatment with 1 mg/kg haloperidol. TH activity in striata from haloperidol-treated rats (haloperidol TH) was increased significantly relative to control when assayed at pH 7.0, but not...

Rybak, A., 1973: Serum magnesium level in chronic alcoholics. Polski Tygodnik Lekarski 28(2): 53-55

Jarrett, R.J.; Keen, H., 1980: Reversal of "mild" diabetes by chlorpropamide. Lancet 1(8183): 1410-1411

MacDougall, R.D.; Koprinarov, I.; Rowlands, J.A., 2008: The x-ray light valve: a low-cost, digital radiographic imaging system--spatial resolution. An x-ray light valve (XLV) coupled with an optical scanner has the potential to meet the need for a low-cost, high quality digital imaging system for general radiography. The XLV/scanner concept combines three well-established, and hence, low-cost...

Tan, S., 2014: Fault and blame, insults to the perinatal brain may be remote from time of birth. There is a certainty in malpractice cases that neurodevelopmental deficits are caused by preventable events at birth when the onset, nature, and timing of the insult in the antenatal and natal period are unknown. The biggest problem is determining...

Starr, A.B.; Gralnick, A., 1955: Factors determining the initial effect of hospitalization on cases of schizophrenia. American Journal of PsychoTherapy 9(2): 243-252

Kimura-Yoshida, C.; Nakano, H.; Okamura, D.; Nakao, K.; Yonemura, S.; Belo, J.A.; Aizawa, S.; Matsui, Y.; Matsuo, I., 2005: Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm. The mouse embryonic axis is initially formed with a proximal-distal orientation followed by subsequent conversion to a prospective anterior-posterior (A-P) polarity with directional migration of visceral endoderm cells. Importantly, Otx2, a homeob...

Noel, S.A., 1994: The changing faces of healthcare: cultural diversity and its impact on access management. Naham Management Journal 20(3): 13-14

Brown, H.E.; Moot, D.J.; Pollock, K.M.; Inch, C., 2000: Dry matter production of irrigated chicory, lucerne and red clover in Canterbury. A field experiment was established at Lincoln University, Canterbury, New Zealand to determine whether chicory, lucerne or red clover (Trifolium pratense) was the most suitable for inclusion in grazing systems as specialist forage. This initial ev...