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Effects of extracts from Panax ginseng, Panax notoginseng and Ligusticum chuanxiong on expression of β-galactosidase and signal pathway p16-cyclin D/CDK-Rb in vascular smooth muscle cells


, : Effects of extracts from Panax ginseng, Panax notoginseng and Ligusticum chuanxiong on expression of β-galactosidase and signal pathway p16-cyclin D/CDK-Rb in vascular smooth muscle cells. Zhong Xi Yi Jie He Xue Bao 10(1): 76-84

To observe the pathological changes of vascular aging in spontaneously hypertensive rats (SHRs), and the effects of extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong on vascular aging. Vascular smooth muscle cells (VSMCs) isolated from thoracic aorta of Wistar-Kyoto (WKY) rats and SHRs were primarily cultured and divided into WKY group, SHR group, valsartan group, low-dose extract group and high-dose extract group. Aging situation of VSMCs was detected by senescence-associated β-galactosidase (SA-β-gal) staining; the cell cycle was analyzed by flow cytometry; mRNA and protein expressions of p16, cyclin D1, cyclin-dependent kinase 4 (CDK4) and retinoblastoma protein (Rb) were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and Western blotting. SA-β-gal stain showed that compared with the WKY group, number of SA-β-gal-positive cells in the SHR group was increased significantly (P<0.01); extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong reduced the number of SA-β-gal-positive cells in the SHR group (P<0.01). Flow cytometry analysis showed that the number of VSMCs in G1 phase was reduced and in S phase was increased significantly (P<0.05) in the SHR group compared with the WKY group, and the number of VSMCs in G(1) phase was increased and in S phase was reduced significantly in the extracts groups (P<0.05). PCR result showed that compared with the WKY group, the mRNA expressions of p16 and Rb in the SHR group were reduced and those of cyclin D1 and CDK4 were increased significantly, the mRNA expressions of p16 and Rb in the extracts group were increased and mRNA expressions of cyclin D1 and CDK4 were reduced significantly. Western blotting result showed that compared with the WKY group, the protein expression of p16 in the SHR group was reduced and the protein expressions of cyclin D1, CDK4 and phospho-Rb were increased significantly (P<0.05), while the protein expression of p16 in the extracts group was increased and the protein expressions of cyclin D1, CDK4 and phospho-Rb were reduced significantly (P<0.05). The extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong can delay vascular aging of SHRs, which works by p16-cyclin D/CDK-RB pathways to inhibit VSMC proliferation.

US$29.90

PMID: 22237278


Other references

Pappas, D.; Matveev, O.I.; Smith, B.W.; Shepard, M.R.; Podshivalov, A.A.; Winefordner, J.D., 2008: Sealed-cell mercury resonance ionization imaging detector. A sealed, compact mercury atomic-absorption resonance ionization imaging detector has been developed and evaluated. The sensitivity of the detector as well as its ability to form two-dimensional images has been demonstrated. Images of faint light...

Cable, B.L.; John, D.T., 1986: Conditions for maximum enflagellation in Naegleria fowleri. Ameba to flagellate transformation in Naegleria fowleri (Lovell strain) was affected by growth temperature, phase of growth, strain of ameba, culture agitation, enflagellation temperature, enflagellation diluent, and cell concentration. Amebae tra...

Gelhen M.; Lochs A., 1990: Quantification of characters from live observations in meiobenthic turbellaria macrostomida. Quantitative estimates of character variability in Microurbellaria are almost completely wanting. This paper describes the within- and between population variability of three closly related marine meiobenthic species of the genus Macrostomum (Macr...

Capella, M.J.; Barraquer, E., 2011: Comparative study of coaxial microincision cataract surgery and standard phacoemulsification. To analyse and quantitatively compare the outcomes of microincisional cataract surgery (CCMIC) with those of conventional phacoemulsification, evaluating the induced astigmatism, endothelial cell count, corneal thickness and foveal thickness in th...

Wortel, J.F.; Wubbels, R.J.; Nuboer, J.F., 1984: Photopic spectral sensitivities of the red and the yellow field of the pigeon retina. The spectral sensitivities of the red field and the yellow field in the retina of the homing pigeon (Columba Livia) were determined on the basis of ERG responses. Between 450 and 550 nm the relative spectral sensitivity of the yellow field turned...

Levi, C.Shani.; Lesmes, U., 2015: Bi-compartmental elderly or adult dynamic digestion models applied to interrogate protein digestibility. The world's population is inevitably ageing thanks to modern progress; however, the development of food and oral formulations tailored to the needs of the elderly is still in its infancy. In vitro digestion models offer high throughput, robus...

Yahaya, N.Haizam.; Teo, R.; Izaham, A.; Tang, S.; Mohamad Yusof, A.; Abdul Manap, N., 2016: Analysis of cricoid pressure application: anaesthetic trainee doctors vs. nursing anaesthetic assistants. To evaluate the ability of anaesthetic trainee doctors compared to nursing anaesthetic assistants in identifying the cricoid cartilage, applying the appropriate cricoid pressure and producing an adequate laryngeal inlet view. Eighty-five participa...

Postic, G.; Ghouzam, Y.; Chebrek, R.; Gelly, J-Christophe., 2017: An ambiguity principle for assigning protein structural domains. Ambiguity is the quality of being open to several interpretations. For an image, it arises when the contained elements can be delimited in two or more distinct ways, which may cause confusion. We postulate that it also applies to the analysis of p...

Jain, J.; Singh, D., 1976: Feed nutrients--a cost study. Indian farming: 26 (2) 28-29

Mauter, M.S.; Elimelech, M.; Osuji, C.O., 2010: Nanocomposites of vertically aligned single-walled carbon nanotubes by magnetic alignment and polymerization of a lyotropic precursor. We demonstrate a novel path for the fabrication of thin-film polymer nanocomposites containing vertically aligned single-walled carbon nanotubes (SWNTs). Liquid crystal mesophases of hexagonally packed cylindrical micelles orient with their long a...