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Effects of low-shear modeled microgravity on a microbial community filtered through a 0.2-μm filter and its potential application in screening for novel microorganisms


, : Effects of low-shear modeled microgravity on a microbial community filtered through a 0.2-μm filter and its potential application in screening for novel microorganisms. Journal of Bioscience and Bioengineering 114(1): 73-79

The effects of low-shear modeled microgravity (LSMMG) on a microbial community filtered through a 0.2-μm filter were investigated, and the potential application of LSMMG in the screening of microorganisms was evaluated. Pond water was passed through a 0.2-μm filter and the filtrate inoculated into two kinds of media (Schneider's insect medium, and ten-times-diluted Schneider's insect [0.1-Sch] medium). The cultures were incubated under LSMMG and normal-gravity and the microbial cell growth rates compared. Cell growth rates, final cell concentrations, and substrate consumption rates were higher in the LSMMG culture than in the normal-gravity culture. The microbial communities obtained under the various culture conditions were subjected to denaturing gradient gel electrophoresis (DGGE), revealing three different groups of microorganisms: (i) microorganisms whose growth rates were increased by LSMMG; (ii) microorganisms whose growth rates were suppressed or inhibited by LSMMG; and (iii) microorganisms whose growth rates were not affected by LSMMG. Sequence analysis of the microorganisms whose growth rates were increased by LSMMG showed that some had high similarity with unculturable microorganisms. When these microorganisms that displayed similarity with unculturable microorganisms were cultivated on agar plates, some of the DGGE bands present in the LSMMG culture were also present. We show that it is possible to isolate and cultivate uncultured microorganisms by using combinations of LSMMG, normal-gravity, and agar plate culturing techniques.

US$19.90

PMID: 22571963

DOI: 10.1016/j.jbiosc.2012.02.021


Other references

Valasek, F.; Visnovsky, J.; Repka, I., 1967: Time and motion study of milk production. Labour efficiency as studied in a 2-row cowshed housing 87 cows; the labour force consisted of 7 persons working in two 7-h shifts. Total working time was 28 min 52 sec/cow/day of which 34.40% were spent moving and 65.60% working without walking,...

Oduro, C.; Antwi Boasiako, C.; Yao, F.C.A., 2001: Visitation assessment of the Accra Zoo from 1987 to 1997. This research was undertaken at the Accra Zoo to assess visitation to the zoo from 1987 to 1997. The aim was to provide a common document from which management can gain new insights into visitations and obtain information for making decisions on t...

El-Sherbini, G.T.; Mohammad, K.A., 2007: Zoonotic cryptosporidiosis in man and animal in farms, Giza Governorate, Egypt. To demonstrated the zoonotic cryptosporidiosis. A total of 189 farmers and their farm animals (200= 71 buffaloes, 59 cows, 43 sheep and 27 goats were examined. Stool samples Each stool sample was subjected to the direct wet smear method and Sheath...

Rames, I., 1992: Nutritional recommendations for adults with diabetes mellitus according to the Czech Diabetology Society. Casopis Lekaru Ceskych 131(7): 221-223

Schneider, A.R.; Eadie, B.J.; Baker, J.E., 2002: Episodic particle transport events controlling PAH and PCB cycling in Grand Traverse Bay, Lake Michigan. To evaluate the influence of episodic events on particle and hydrophobic organic contaminant (HOC) cycling in the Great Lakes, we deployed sequencing sediment traps at two locations in the western arm of Grand Traverse Bay, Lake Michigan. The trap...

Reznik, E.; Christodoulou, D.; Goldford, J.E.; Briars, E.; Sauer, U.; Segrè, D.; Noor, E., 2017: Genome-Scale Architecture of Small Molecule Regulatory Networks and the Fundamental Trade-Off between Regulation and Enzymatic Activity. Metabolic flux is in part regulated by endogenous small molecules that modulate the catalytic activity of an enzyme, e.g., allosteric inhibition. In contrast to transcriptional regulation of enzymes, technical limitations have hindered the product...

Bambino F.; Brownlee R.T.C.; Chiu F.C.K., 1991: Facile synthesis of protected dipeptide acids containing carboxyl terminal alpha alpha dialkyl amino acids. Tetrahedron Letters 32(28): 3407-3408

Spelsberg T.C., 1983: A rapid method for analysis of ligand binding to dna and soluble nucleo proteins using streptomycin application to steroid receptor ligands. A method is described which allows the rapid analysis of the binding of practically any molecules to DNA or to protein-DNA complexes (termed nucleoacidic protein or NAP). The antibiotic streptomycin sulfate, a soluble aminoglycoside, is used to pr...

Boer, G.J., 1993: Climate change and the regulation of the surface moisture and energy budgets. The increase in the vigour of the hydrological cycle simulated in a 2 x CO2 experiment with the Canadian Climate Centre general circulation model (CCC GCM) is smaller than that obtained by other models which have similar increases in mean surface...

Anonymous, 1965: Seychelles. An economic survey. An account is given of the main geographic, political, climatic, demographic and economic features of this UK Crown Colony. Agriculture is the main activity, providing direct employment for 40 per cent. of the working population (1960 census), wit...