+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes

, : Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Molecular Biology of the Cell 23(20): 3927-3935

Tail-anchored (TA) proteins have a single C-terminal transmembrane domain, making their biogenesis dependent on posttranslational translocation. Despite their importance, no dedicated insertion machinery has been uncovered for mitochondrial outer membrane (MOM) TA proteins. To decipher the molecular mechanisms guiding MOM TA protein insertion, we performed two independent systematic microscopic screens in which we visualized the localization of model MOM TA proteins on the background of mutants in all yeast genes. We could find no mutant in which insertion was completely blocked. However, both screens demonstrated that MOM TA proteins were partially localized to the endoplasmic reticulum (ER) in spf1 cells. Spf1, an ER ATPase with unknown function, is the first protein shown to affect MOM TA protein insertion. We found that ER membranes in spf1 cells become similar in their ergosterol content to mitochondrial membranes. Indeed, when we visualized MOM TA protein distribution in yeast strains with reduced ergosterol content, they phenocopied the loss of Spf1. We therefore suggest that the inherent differences in membrane composition between organelle membranes are sufficient to determine membrane integration specificity in a eukaryotic cell.


PMID: 22918956

DOI: 10.1091/mbc.E11-12-0994

Other references

Bauereisen, E., 1963: On The Problem Of Specificity Of Mesenteric Circulation Reflexes. Zeitschrift für Biologie 114: 81-88

Boo K.S.; Shinn H.C.; Han M.W.; Lee M.H., 1990: Initiation and termination of pupal diapause in the oriental tobacco budworm heliothis assulta. Laboratory and field studies were conducted to investigate the initiation and termination factors for pupal diapause in the Oriental tobacco budworm, Heliothis assulta Guenee. Diapause induction was dependent on photoperiod and temperature experie...

Correia, H.S.; Batista, M.Teresa.; Dinis, T.C.P., 2007: The activity of an extract and fraction of Agrimonia eupatoria L. against reactive species. Agrimonia eupatoria L. (agrimony) is a medicinal plant largely used in traditional medicine. Recently, phytochernical studies on an agrimony hydro-alcoholic extract and a polyphenol-enriched fraction obtained from it were carried out. The fractio...

Klasz, W., 1981: Systematic planning of advisory programmes? Experiences with the use of specific management methods in advisory work in Austria - 3rd part, conclusion. This concluding part of the paper deals with the following questions: (1) (a) can or should the new methods in the shared, systematic working out of aims, measures, solutions to problems etc. also be used in the planning and carrying out of adviso...

Kim, K-pyo.; Ahn, J-Hee.; Kim, S-Bae.; Jung, K.Hae.; Yoon, D.Hyun.; Lee, J.Shin.; Ahn, S-Hyun., 2012: Prospective evaluation of the drug-metabolizing enzyme polymorphisms and toxicity profile of docetaxel in Korean patients with operable lymph node-positive breast cancer receiving adjuvant chemotherapy. Inter-individual variability of pharmacokinetics may account for unpredictable toxicities of docetaxel.From March 27 to June 28, female patients with operable lymph node-positive breast cancer receiving docetaxel-containing adjuvant chemotherapy w...

Gastaut, H., 1969: Classification of the epilepsies. Proposal for an international classification. Epilepsia 10: Suppl:14-21

Downing, P., 1979: Interpretation and research. A brief description of the work and aims of the Dartington Amenity Research Trust in the UK.

Thompson, A.J.; Cronin, M.S.; George, A.; Yu, J.; Cerullo, J.L.; Orlova, C.; Inouye, H.; Kirschner, D.A., 2001: Biochemical and structural studies on P0 glycoprotein and PNS myelin of Xenopus. Biophysical Journal 80(1 Part 2): 52a, January

Koshy, S.; Tandon, S., 1998: Dental age assessment: the applicability of Demirjian's method in south Indian children. The paper concerns the testing of Demirjian's method of age assessment in South Indian children. Since previous studies have shown the inapplicability of Demirjian's method on other populations, an attempt to compile a maturity standard...

V.V.Oganesyan, 2011: On the relation between the temperature anomaly duration and the value of its extrema. A statistical structure of the time series of air temperature with a daily resolution is studied from the data of nine meteorological stations of Russia for the period from 1961 to 26. A quantitative relation is obtained between the temperature an...