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Evaluation of early stage human bone marrow stromal proliferation, cell migration and osteogenic differentiation on μ-MIM structured stainless steel surfaces


, : Evaluation of early stage human bone marrow stromal proliferation, cell migration and osteogenic differentiation on μ-MIM structured stainless steel surfaces. Journal of Materials Science. Materials in Medicine 24(5): 1285-1292

It is well established that surface topography greatly affect cell-surface interactions. In a recent study we showed that microstructured stainless steel surfaces characterized by the presence of defined hexagonally arranged hemisphere-like structures significantly affected cell architecture (shape and focal adhesion size) of primary human bone mesenchymal stromal cells. This study aimed at further investigating the influence these microstructures (microcline protruding hemispheres) on critical aspects of cell behaviour namely; proliferation, migration and osteogenic differentiation. As with previously reported data, we used primary human bone mesenchymal stromal cells to investigate such effects at an early stage in vitro. Cells of different patients were utilised for cell migration studies. Our data showed that an increase in cell proliferation was exhibited as a function of surface topography (hemispheres). Cell migration velocity also varied as a function of surface topography on patient specific basis and seems to relate to the differentiated state of the seeded cell population (as demonstrated by bALP positivity). Osteogenic differentiation, however, did not exhibit significant variations (both up and down-regulation) as a function of both surface topography and time in culture.

US$19.90

PMID: 23386209

DOI: 10.1007/s10856-013-4876-7


Other references

Chelidze, G.F., 1953: Stratigraphy of Miocene deposits of the anticline fauna of Urt. Soobshcheniya Akademii Nauk Gruzinskoi SSR: 14: 19-25

Dale, B.; Fiorentino, A.; D.S.efano, R.; D.M.tteo, L.; D.S.mone, M.L.; Wilding, M.; Zullo, F., 1999: Pregnancies after activated oocyte transfer: a new option for infertility treatment. In this preliminary report, we describe a new technique involving the same-day transfer of activated oocytes to the uterus after intracytoplasmatic sperm injection (ICSI). The technique, termed activated oocyte transfer (AOT), offered to 19 couple...

Rudner, R.; Lin, H.J.; Hoffmann, E.M.; Chargaff, E., 1967: Studies on the loss and the restoration of the transforming activity of the deoxyribonucleic acid of Bacillus subtilis. DNA preparations of B. subtilis, were assayed for transforming activity for three unlinked genetic markers (adenineleucine methionine in different stages of de- and renaturation induced by the gradual withdrawal of electrolytes by dialysis and the...

Boue, A., 1950: The horse of Oued Guir (the Small Barb of Oran). THE valley of the Guir is situated some 60 miles S. W. of Colomb-Bechar, near the southern border of French Morocco. The horse bred there is a variety of the Barb breed; it conforms to the moro slenderly built Oran type, as opposed to the heavier...

Renshaw, G.D., 1976: The distribution of trace elements in human hair and its possible effect on reported elemental concentration levels. Medicine, Science, and the Law 16(1): 37-39

Damsteegt, V.D., 1983: Maize streak virus 1. host range and vulnerability of maize zea mays germ plasm. Grass accessions (138), maize hybrids (52%), inbreds, exotic lines and sweet corn cultivars, several Sorghum, Tripsacum and Zea spp., and major cereal crop cultivars were tested for susceptibility to maize streak virus disease in both seedling and...

Popkhristov, A.; Obreshkov, K.; Denovski, D., 1962: Studies on the role of vitamins A and D in the immunogenesis of fowl cholera and Newcastle disease.. Two-month-old fowls immunized with the Haifa strain of Newcastle disease virus or a killed aluminium-adsorbed fowl cholera vaccine showed greater resistance to challenge when fed vitamins A and D or green food.

de S Santos Machado, V.; Valadares, A.Lúcia.Ribeiro.; Costa-Paiva, Lúcia.H.; Osis, M.J.; Sousa, M.H.; Pinto-Neto, Aão.M., 2014: Aging, obesity, and multimorbidity in women 50 years or older: a population-based study. This study aims to evaluate multimorbidity and its associated factors in Brazilian women aged 50 years or older. This is a cross-sectional, population-based study using self-reports. A total of 622 women aged 50 years or older were included. Multi...

Ebert T.A.; Russell M.P., 1988: Latitudinal variation in recruitment of strongylocentrotus purpuratus the role of mesoscale oceanographic processes. Burke, R D , Et Al (Ed ) Echinoderm Biology; Sixth International Echinoderm Conference, Victoria, British Columbia, Canada, August 23-28, 1987 Xiii+818p A A Balkema: Rotterdam, Netherlands, Illus Maps 795

Sharma, B.D.; Hore, D.K.; Dobhal, V.K., 1996: Classificatory analysis in ricebean (Vigna umbellata). The genetic diversity of 28 genotypes of Vigna umbellata of indigenous and exotic origin was studied by subjecting data on 9 yield components to Mahalanobis' Dsuperscript 2 analysis. The genotypes were grouped into 6 clusters. Clusters II, II...