+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of skin regeneration after burns in vivo and rescue of cells after thermal stress in vitro following treatment with a keratin biomaterial

, : Evaluation of skin regeneration after burns in vivo and rescue of cells after thermal stress in vitro following treatment with a keratin biomaterial. Journal of Biomaterials Applications 29(1): 26-35

Thermal burns typically display an injury pattern dictated by the transfer of the thermal energy into the skin and underlying tissues and creation of three zones of injury represented by a necrotic zone of disrupted cells and tissue, an intermediate zone of injured and dying cells, and a distant zone of stressed cells that will recover with proper treatment. The wound healing capabilities of a keratin biomaterial hydrogel were studied in two pilot studies, one using a chemical burn model in mice and the other a thermal burn model in swine. In both studies, keratin was shown to prevent enlargement of the initial wound area and promote faster wound closure. Interestingly, treating thermally stressed dermal fibroblast in culture demonstrated that soluble keratin was able to maintain cell viability and promote proliferation. Separation of so-called alpha and gamma fractions of the keratin biomaterial had differential effects, with the gamma fraction producing more pronounced cell survival and recovery. These results suggest that the gamma fraction, composed essentially of degraded alpha keratin proteins, may facilitate cell rescue after thermal injury. Treatment of burns with gamma keratin may therefore represent a potential therapy for wounds with an intermediate zone of damaged tissue that has the potential to contribute to spontaneous healing.


PMID: 24272161

DOI: 10.1177/0885328213513310

Other references

Li, J.; Shou, J.; Guo, Y.; Tang, Y.; Wu, Y.; Jia, Z.; Zhai, Y.; Chen, Z.; Xu, Q.; Wu, Q., 2016: Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. The human genome contains millions of DNA regulatory elements and a large number of gene clusters, most of which have not been tested experimentally. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease...

Mahantshetty, U.; Banerjee, S.; Kakkar, S.; Murthy, V.; Bakshi, G.; Tongaonkar, H.B.; Shrivastava, S., 2013: Treatment of Stage I Seminoma testis with extended field adjuvant radiation. With an aim to analyze and document the outcome of Stage I Seminoma patients we undertook a retrospective analysis of patients treated between January 1990 and December 1998. The treatment charts of patients treated between January 1990 and Decemb...

Myers, A.H.; Baker, S.P.; Li, G.; Smith, G.S.; Wiker, S.; Liang, K.Y.; Johnson, J.V., 1999: Back injury in municipal workers: a case-control study. Objectives. The purpose of this study was to identify factors associated with acute low back injury among municipal employees of a large city. Methods. For each of 200 injured case patients, 2 coworker controls were randomly selected, the first ma...

Tomei, G.; Ciarrocca, M.; Capozzella, A.; Fiaschetti, M.; Tomao, E.; Cangemi, C.; Rosati, M.Valeria.; Cerratti, D.; Anzani, M.Francesca.; Pimpinella, B.; Monti, C.; Tomei, F., 2008: Hemopoietic system in traffic police exposed to urban stressors. The aim of the study is to evaluate if occupational exposure to urban stressors could cause alterations in red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean cell volume (MCV), mean cell hemoglobin (MCH), mean corpuscular hemoglobin c...

Rosenwirth, B.; Griengl, H.; Wanek, E.; D.C.ercq, E., 1985: 5-(2-Chloroethyl)-2'-deoxyuridine: a potent and selective inhibitor of herpes viruses in vitro and in vivo. Antiviral Research Suppl 1: 20-28

Fariss M.W.; Blanke R.V.; Boylan J.J.; King S.T.; Guzelian P.S., 1978: Reductive bio transformation f chlordecone in man and rat. Toxicology & Applied Pharmacology 45(1): 337

Singh, B.P.; Das, M.; Ram, M.; Dwivedi, B.S.; Prasad, R.N., 1990: Critical limit of phosphorus in soils for groundnut (Arachis hypogaea) grown in acid Alfisols in Meghalaya. Results of pot experiments show that the critical P concentrations below which economic responses to P applications could be expected from groundnut in acid Alfisols of Meghalaya are 6.5 ppm available P in soils and 0.17% in shoots.

Smith, R.K.W.; Goodship, A.E., 2001: The influence of ageing and exercise on tendon matrix Hypotheses for the initiation and prevention of strain-induced tendinopathies. Tendon strain injuries are common in human and equine athletes. In horses, in vitro strain measurements at rupture are close to in vivo measurements at the gallop. Small decreases in matrix quality will therefore increase the risk of tendinopathy...

Connolly, A.M.; Wilkinson, D.; Harrison, A.; Lurie, M.; Karim, S.S., 1999: Inadequate treatment for sexually transmitted diseases in the South African private health sector. Correct management of sexually transmitted diseases (STDs) is important for their control, and to reduce HIV transmission. Guidelines on syndromic management of STDs were introduced by the provincial Department of Health in KwaZulu/Natal (KZN) in...

Eckerlin, R.P.; Leigh, W.H., 1962: Platynosomum fastosum Kossack, 1910 (Trematoda: Dicrocoeliidae) in South Florida. Eckerlin & Leigh report natural infections of Platynosomum fastosum from 6 of 19 cats and 7 of 17 Didelphys marsupialis virginiana in southern Florida. The opossum is a new host record. Subulina octona is the molluscan host. The second intermediat...