geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of skin samples for bovine viral diarrhea virus by use of reverse transcriptase polymerase chain reaction assay after vaccination of cattle with a modified-live bovine viral diarrhea virus vaccine


, : Evaluation of skin samples for bovine viral diarrhea virus by use of reverse transcriptase polymerase chain reaction assay after vaccination of cattle with a modified-live bovine viral diarrhea virus vaccine. American Journal of Veterinary Research 73(2): 319-324

To determine whether vaccine virus can be detected by use of reverse transcriptase (RT)-PCR assays for pooled and individual skin samples obtained from cattle after vaccination with a commercially available modified-live bovine viral diarrhea virus (BVDV) vaccine. 12 BVDV-seropositive steer calves and 7 BVDV-seronegative (antibody titer < 1:4) heifers; all cattle were free of persistent infection with BVDV. 2 experiments were conducted. Cattle were vaccinated on day 0 with a commercially available modified-live BVDV vaccine. Skin samples were collected on days 0, 3 to 14, 16, and 18 for virus detection by use of RT-PCR assay on individual and pooled samples. In addition, blood samples and nasal swab specimens were collected for virus isolation. All cattle, regardless of serologic status, had negative results for BVDV as determined by use of RT-PCR assay of individual and pooled skin samples. Virus was detected via virus isolation in serum or the buffy coat in 5 of 7 heifers that were seronegative when vaccinated. These findings indicated that it would be unlikely to detect BVDV vaccine virus in skin by use of RT-PCR assay of individual or pooled skin samples obtained from cattle after vaccination with a commercially available modified-live BVDV vaccine. Veterinarians and producers should be confident that positive test results for BVDV on skin samples would not likely be caused by the vaccination virus after administration of a modified-live virus vaccine.

US$19.90

PMID: 22280397

DOI: 10.2460/ajvr.73.2.319


Other references

Kolodziejska, J., 2005: Wplyw Hydrokoloidow Stosowanych W Recepturze Past Do Zebow Na Ich Wlasciwosci Reologiczne I Dostepnosc Farmaceutyczna Jonu Fluorkowego. Various formulations for tooth pastes with gel vehicles based on natural polysaccharides and modified cellulose derivatives were developed. Extensibility of model formulations was assessed by extensometry, while their viscosity was assayed by rheo...

Harris Leonard D., 1963: Thrusting developed after folding in the Valley and Ridge Province, Southwest Virginia. In the Cumberland overthrust block, major bedding-plane thrust have been interpreted as developing early in the orogenic cycle, when the rocks were little deformed. Recent detail mapping in and adjacent to the block indicates that thrusting occurr...

Gregor, A.; Macbeth, F.R.; Paul, J.; Cram, L.; Hansen, H.H., 1993: Radical radiotherapy and chemotherapy in localized inoperable non-small-cell lung cancer: a randomized trial. Journal of the National Cancer Institute 85(12): 997-999

Mcnamara, P.; Lawson, J.A.; Rokach, J.; Fitzgerald, G.A., 1999: PPAR activation by isoprostanes A signaling mechanism for oxidant injury. Circulation 100(18 SUPPL ): I 480-I 481, Nov 2

Swinfen, Roger., 1998: Devon ringing report 1996. Devon Bird Report. 1996( ); 69: 100-108

Baudry, D.; Cabanis, M-Odile.; Patte, C.; Zucker, J-Michel.; Pein, Fçois.; Fournet, J-Christophe.; Sarnacki, S.; Junien, C.; Jeanpierre, Cécile., 2003: Cadherins in Wilms' tumor: E-cadherin expression despite absence of WT1. Loss of heterozygosity of chromosome 16q occurs in 17-25% of Wilms' tumors. Two cadherin genes mapping to 16q22 were chosen as candidate genes: E-CAD, encoding epithelial cadherin, because it is involved in kidney development and it was recen...

Berkman, N.; Meirow, D.; Katzir, Z.; Bar-On, H., 1993: Acute renal failure due to carnitine palmitoyltransferase deficiency. A young, apparently healthy, soldier developed acute muscle weakness and rhabdomyolysis following prolonged exercise. The resultant myoglobinuria caused severe acute renal failure. Further investigation revealed the presence of carnitine palmitoyl...

Lafer, G.S.; Nilsson, A.N.; Kholin, S.K., 1997: Additional records and new synonyms of Cicindelidae and Carabidae from the Island of Sakhalin in the Russian Far East. Faunistic records of three cicindelid and 47 carabid beetle species are given for the Island of Sakhalin in the Russian Far East. Most records refer to a material collected in 1993 and 1994 in SW Sakhalin by the two junior authors. The following f...

Valldor, P.; Miethling-Graff, R.; Martens, R.; Tebbe, C.C., 2016: Fate of the insecticidal Cry1Ab protein of GM crops in two agricultural soils as revealed by ¹⁴C-tracer studies. Insecticidal delta-endotoxins of Bacillus thuringiensis are among the most abundant recombinant proteins released by genetically modified (GM) crops into agricultural soils worldwide. However, there is still controversy about their degradation and...

Ishaaya, I.; Holmstead, R.L.; Casida, J.E., 1977: Triphenyl derivatives of group IV elements as inhibitors of growth and digestive enzymes of Tribolium castaneum larvae. The potency of triphenyl derivatives of group IV elements in inhibiting the growth of 1st and 4th instar T. castaneum larvae is much greater for Ph3SnCl[phenyltin trichloride] and Ph3PbCl [triphenyl lead chloride] than for Ph3GeCl [triphenyl germa...