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Experimental factorial design applied to mucoadhesive lipid nanoparticles via multiple emulsion process

, : Experimental factorial design applied to mucoadhesive lipid nanoparticles via multiple emulsion process. Colloids and Surfaces. B, Biointerfaces 100: 84-89

Solid lipid nanoparticles (SLNs) produced from multiple emulsions technology theoretically enclose an inner aqueous compartment suitable for hydrophilic biomolecules. This paper reports a 3(3) full factorial design study to optimize SLNs formulations for hydrophilic biomolecules. The concentrations of solid lipid, lipophilic and hydrophilic emulsifiers were set as the 3 independent variables. Mean particle size (Z-Ave), polydispersity index (PI) and zeta potential (ZP) were set as the dependent variables. The selected optimized parameters were set as 1.0 wt% of solid lipid, 0.25 wt% of lipophilic emulsifier and 1.5 wt% of hydrophilic emulsifier. The coating of SLNs with sodium alginate was found to improve the ZP of the lipid particles and these results suggest that the ideal concentration was 0.75 wt%. The influence of low pH (i.e., about 2-3) in the inner aqueous phase was stronger than higher pH values, contributing for the production of larger droplet sizes. Nevertheless, these systems can be useful for the incorporation of biomolecules requiring a pH ranging between 4 and 10. SLNs based on multiple emulsions technology were found to be a promising approach for the incorporation of several hydrophilic drugs, such as proteins and peptides.


PMID: 22766285

DOI: 10.1016/j.colsurfb.2012.04.014

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