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Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system


, : Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system. Peptides 61: 56-60

The nociceptin receptor (NOP) and its ligand nociceptin/orphanin FQ (N/OFQ) have been shown to exert a modulatory effect on immune cells during sepsis. We evaluated the suitability of an experimental lipopolysaccharide (LPS)-induced sepsis model for studying changes in the nociceptin system. C57BL/6 mice BALB/c mice and Wistar rats were inoculated with different doses of LPS with or without a nociceptin receptor antagonist (UFP-101 or SB-612111). In C57BL/6 mice LPS 0.85 mg/kg injection produced no septic response, whereas 1.2mg/kg produced a profound response within 5h. In BALB/c mice, LPS 4 mg/kg produced no response, whereas 7 mg/kg resulted in a profound response within 24h. In Wistar rats LPS 15 mg/kg caused no septic response in 6/10 animals, whereas 25mg/kg resulted in marked lethargy before 24h. Splenic interleukin-1β mRNA in BALB/c mice, and serum TNF-α concentrations in Wistar rats increased after LPS injection in a dose-dependent manner, but were undetectable in control animals, indicating that LPS had stimulated an inflammatory reaction. IL-1β and TNF-α concentrations in LPS-treated animals were unaffected by administration of a NOP antagonist. Similarly NOP antagonists had no effect on survival or expression of mRNA for NOP or ppN/OFQ (the N/OFQ precursor) in a variety of tissues. In these animal models, the dose-response curve for LPS was too steep to allow use in survival studies and no changes in the N/OFQ system occurred within 24h. We conclude that LPS-inoculation in rodents is an unsuitable model for studying possible changes in the NOP-N/OFQ system in sepsis.

US$19.90

PMID: 25161013

DOI: 10.1016/j.peptides.2014.08.009


Other references

Ueland, B.G.; Lau, G.C.; Cava, R.J.; O'Brien, J.R.; Schiffer, P., 2006: Slow spin relaxation in a highly polarized cooperative paramagnet. We report measurements of the ac susceptibility of the cooperative paramagnet Tb2Ti2O7 in a strong magnetic field. Our data show the expected saturation maximum in chi(T) and also an unexpected frequency dependence of this peak at low frequencies...

Gilmore, H.J.; Liu, A.; Koester, K.Ann.; Amico, K.Rivet.; McMahan, V.; Goicochea, P.; Vargas, L.; Lubensky, D.; Buchbinder, S.; Grant, R., 2013: Participant experiences and facilitators and barriers to pill use among men who have sex with men in the iPrEx pre-exposure prophylaxis trial in San Francisco. In 2010, the iPrEx study demonstrated efficacy of daily emtricitabine/tenofovir disoproxil fumarate (FTC/TDF) pre-exposure prophylaxis (PrEP) in reducing HIV acquisition among men who have sex with men. Adherence to study product was critical for...

Yu, X.; Murao, K.; Imachi, H.; Cao, W-Ming.; Li, J.; Matsumoto, K.; Nishiuchi, T.; Ahmed, R.A.M.; Wong, N.C.W.; Kosaka, H.; Unterman, T.G.; Ishida, T., 2007: Regulation of scavenger receptor class BI gene expression by angiotensin II in vascular endothelial cells. High-density lipoprotein mediates a normal physiological process called reverse cholesterol transport. In this process, a scavenger receptor of the B class (SR-BI)/human homologue of SR-BI, CD36, and LIMPII analogous-1 (hSR-BI/CLA-1) facilitates...

Nikishin, V.P., 2003: Ultrastructure of the surface complex of acanthocephalans with the example of Filicollis anatis (Palaeacanthocephala: Polymorphidae) and Neoechinorhynchus crassus (Eoacanthocephala: Neoechinorhynchidae). Fine morphology of external tegument (surface complex) of young and mature acanthocephalans Filicollis anatis and young Neoechinorhynchus crassus is described. Based on comparative analysis of the obtained results and literature data, the surface...

Novak, J.M.; Watts, D.W., 1996: Solid-phase extraction and GC analyses of select agricultural pesticides and metabolites in stream water. The occurrence of agricultural pesticides in surface waters around the USA has created a concern over the status of safe drinking water. Solid-phase extraction (SPE) or liquid-liquid extraction (LLE) is usually employed to concentrate trace levels...

Ravagnan, R.; D'Avila, E., 1966: The effectiveness of external cardiac massage. Presentation of 10 cases. Minerva Anestesiologica 32(10): 553-554

Quick, J.B., 1953: Improved efficiency in respiration during pediatric anesthesia as a means of controlling acidosis. Journal of the American Osteopathic Association 53(2): 147-149

Duarte, A.F.; Cruz, M.J.; Baudrier, T.; Mota, A.; Azevedo, F., 2009: Unilateral laterothoracic exanthem and primary Epstein-Barr virus infection: case report. Unilateral laterothoracic exanthem is a self-limited disease that occurs most commonly in children. It is characterized by unilateral exanthem, often in axillary region. The etiology is unknown, but a viral agent is suspected. We report a 1-year-o...

Dias D.S.lva, V.Jose.; Gnecchi-Ruscone, T.; Lavelli, B.; Bellina, V.; Manzella, D.; Porta, A.; Malliani, A.; Montano, N., 2002: Opposite effects of iv amiodarone on cardiovascular vagal and sympathetic efferent activities in rats. It is unknown whether amiodarone exerts a direct central action on the cardiovascular autonomic nervous system. This study was designed to evaluate the effects of acute amiodarone administration on vagal and sympathetic efferent nerve discharges....

Pierozzi, N.I.abel; Baroni, R.M.ro; Benatti, R., 2008: Cytological investigations of the microsporogenesis in male-sterile ramie (Boehmeria nivea Gaud.) and its offspring. CYTOLOGIA 73(1): 21-31