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Expression of C1q complement component in Barrett's esophagus and esophageal adenocarcinoma


, : Expression of C1q complement component in Barrett's esophagus and esophageal adenocarcinoma. Journal of Gastrointestinal Surgery 14(8): 1207-1213

C1q, an element of the first component of complement, is known to be expressed by interdigitating and follicular dendritic cells in the spleen, where it has been suggested that C1q is involved in capturing immune complexes. The present study investigated whether C1q is expressed in Barrett's esophagus and esophageal adenocarcinoma and, if so, whether its expression is associated with dendritic cells. Endoscopic biopsy or operative surgical specimens were obtained from 15 patients with Barrett's esophagus, 13 patients with esophageal adenocarcinoma and 12 patients whose biopsy specimens did not show the presence of specialized intestinal metaplasia or adenocarcinoma. Barrett's esophagus was diagnosed by the presence of a macroscopic area of columnar-lined esophagus as well as microscopic intestinal metaplasia with goblet cells. Immunohistochemistry utilizing anti-C1q and markers for dendritic cells and macrophages was performed on sections of tissue samples embedded in paraffin. Double immunostaining with C1q/CD83 and C1q/CD68 was used to analyze the possible co-localization of C1q with dendritic cells and macrophages. The expression of C1q by dendritic cells and macrophages was also examined in in vitro studies using reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. In all specimens studied, C1q expression was detected as being distributed irregularly throughout the lamina propria. A computerized quantitative analysis showed that C1q expression was significantly higher in tissue specimens without specialized intestinal-type metaplasia than in Barrett's esophagus specimens and specimens with adenocarcinoma. Double immunostaining revealed that dendritic cells and macrophages expressed C1q in all analyzed esophageal specimens. The expression of C1q by dendritic cells and macrophages was also demonstrated in in vitro studies using RT-PCR and Western blotting. The findings suggest that reduced levels of the expression of C1q by dendritic cells and macrophages in the esophagus may play a role in the formation of immune responses associated with the formation of specialized intestinal metaplasia and the development of adenocarcinoma.

US$19.90

PMID: 20496011

DOI: 10.1007/s11605-010-1230-y


Other references

Helfrich, W., 1995: Tension-induced mutual adhesion and a conjectured superstructure of lipid membranes. Lipowsky, R [Editor], Sackmann, E [Editor] Handbook of Biological Physics, Vol 1; Structure and dynamics of membranes: From cells to vesicles (Part A): Generic and specific interactions (Part B) 691-721

Laus, Rério.; Costa, T.G.; Szpoganicz, B.; Fávere, V.T., 2010: Adsorption and desorption of Cu(II), Cd(II) and Pb(II) ions using chitosan crosslinked with epichlorohydrin-triphosphate as the adsorbent. In this study, chitosan (CTS) was crosslinked with both epichlorohydrin (ECH) and triphosphate (TPP), by covalent and ionic crosslinking, respectively. The resulting new CTS-ECH-TPP adsorbent was characterized by CHN analysis, EDS, FTIR spectrosco...

Bruning, E.J., 1955: Pathogenesis and significance of intra-arterial foreign body embolisms of the lung in children. Virchows Archiv für Pathologische Anatomie und Physiologie und für Klinische Medizin 327(4): 460-479

Catterall, W.A., 1986: Molecular properties of voltage-sensitive sodium channels. Prevalence and titer of total, free, and bound cerebrospinal fluid anti-myelin basic protein (MBP) antibodies as well as free/bound ratios were determined in four groups of patients with multiple sclerosis (MS) and three groups of controls. All pa...

Scheff, Stephen William, 2001: Protection against traumatic brain injury. The present invention relates to therapeutic uses of cyclosporin A to reduce adverse effects of neural injury.

Ben-Jaballah, N.; Mnif, K.; Bouziri, A.; Kazdaghli, K.; Belhadj, S.; Zouari, B., 2005: High-frequency oscillatory ventilation in paediatric patients with acute respiratory distress syndrome - early rescue use. In order to determine the response to high-frequency oscillatory ventilation (HFOV), used as an "early rescue'' therapy, in a cohort of paediatric patients with acute respiratory distress syndrome ( ARDS), a prospective clinical stu...

Gannon, B.M.; Reichard, E.E.; Fantegrossi, W.E., 2015: Psychostimulant Abuse and HIV Infection: cocaine, methamphetamine, and "bath salts" cathinone analogues. NlmCategory="UNASSIGNED">Psychostimulants are among the most widely-abused substances worldwide, and typically exert their abuse-related effects via interactions with monoamine reuptake transporters within the CNS. Over the last decad...

Pilarczyk, B.; Balicka Ramisz, A.; Ramisz, A.; Pajak, B., 2004: Influence of dermanyssus gallinae on health and production in layers. Aim of the study was to estimate the influence of Dermanyssus gallinae on the health and production of layers. The study was carried out in a layer cross-breeding (HYLINE) farm of 17,650 birds. The material for investigation was collected from dif...

Potapov, I.I., 1957: Clinical aspects and surgical treatment of benign tumors of the pharynx and of the external laryngeal ring. Vestnik Otorinolaringologii 19(4): 8-17

Lang, J.; Samii, A., 1991: Retrosigmoidal approach to the posterior cranial fossa. An anatomical study. An anatomical study was performed in order to obtain help for orientation regarding the retrosigmoid approach and its osteoclastic craniotomy. The insertions of the sternocleidomastoid, the splenius capitis, the longissimus capitis and the obliquu...