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External quality assessment for the determination of diphtheria antitoxin in human serum


, : External quality assessment for the determination of diphtheria antitoxin in human serum. Clinical and Vaccine Immunology 17(8): 1282-1290

Accurate determination of diphtheria toxin antibodies is of value in determining the rates of immunity within broad populations or the immune status of individuals who may be at risk of infection, by assessing responses to vaccination and immunization schedule efficacy. Here we report the results of an external quality assessment (EQA) study for diphtheria serology, performed within the dedicated surveillance network DIPNET. Twelve national laboratories from 11 European countries participated by testing a standard panel of 150 sera using their current routine method: Vero cell neutralization test (NT), double-antigen enzyme-linked immunosorbent assay (ELISA; DAE), dual double-antigen time-resolved fluorescence immunoassay (dDA-DELFIA), passive hemagglutination assay (PHA), toxin binding inhibition assay (ToBI), and in-house or commercial ELISAs. The objective of the study was not to identify the best assay, as the advantages and drawbacks of methods used were known, but to verify if laboratories using their routine method would have categorized (as negative, equivocal, or positive) a serum sample in the same way. The performance of each laboratory was determined by comparing its results on a quantitative and qualitative basis to NT results from a single reference laboratory, as this test is considered the in vitro "gold standard." The performance of laboratories using NT was generally very good, while the laboratories' performance using other in vitro methods was variable. Laboratories using ELISA and PHA performed less well than those using DAE, dDA-DELFIA, or ToBI. EQA is important for both laboratories that use in vitro nonstandardized methods and those that use commercial ELISA kits.

US$19.90

PMID: 20610661

DOI: 10.1128/CVI.00096-10


Other references

Gilby J.L.; Yuen C.M.K.; Aston T.R.C., 1988: Geotechnical monitoring of tunnel boring machine drivages at the Donkin-Morien Mine, Nova Scotia. CIM Bulletin (1974) 81(915): 60-72

Oliver, J., 1971: An introduction to dairying in Rhodesia. (Occ. Pap. Dep. Agric. Univ. Rhod. 3). This manual sets out the basic requirements for dairy production in the tropics in clear and simple terms, including chapters on: Milk and milk products (pp. 1-11, with regard to composition and value in human nutrition); Factors that affect the a...

Kubo A., 1970: Gardneria d alkaloids part 5 on the c d ring cleavage reaction of sarpagine skeleton. Yakugaku Zasshi 90(2): 224-229

Kim, S.Kyoung.; Kim, B.Ra.; Kim, K.; Kim, S.; Jung, J.Hwa.; Hahm, J.Ryeal.; Jung, J., 2016: Dramatic Improvement of Diabetes Mellitus Following the Treatment of Coexisting Acromegaly and Cushing's Syndrome. Endocrine diseases are frequently accompanied by diabetes mellitus and treatment of an underlying endocrine disease often improves glucose control. The co-occurrence of acromegaly and Cushing's syndrome is extremely rare. We herein describe a...

Haumann, B.F., 1991: European firms face new challenges. The European oleochemical industry (producing fatty acids, fatty acid methyl esters, fatty alcohols, fatty amines and natural glycerine) represents more than one-third of world oleochemical production. The paper maintains that the industry is faci...

Russo J.Jr; Russo M.E., 1982: Comparative review of 2 new wide spectrum penicillins mezlocillin and piperacillin. Clinical Pharmacy 1(3): 207-216

Taylor, R.-W.M.auley, K.-A.B.rbezat, W.; Strong, A.; Williams, S.-M.M.nn, J., I., 2007: APPLE Project: 2-y findings of a community-based obesity prevention program in primary school-age children. Background: Developing effective strategies for obesity prevention in children is urgently required.Objective: We determined the effectiveness of a 2-y controlled community-based intervention to prevent excessive weight gain in 5-12-y-old children...

Kaplan, D.L., 1993: Biblical leprosy: an anachronism whose time has come. Although there is a growing consensus that biblical leprosy was indeed not Hansen's disease, there has been no agreement as to the disease or diseases described in the Bible. Biblical scholars have recently provided valuable insights regardin...

Pagel, W.; Hall, S., 2010: Aspiration type of congenital tuberculosis. Tubercle 27: 153-158

Obreja, O.; Hirth, M.; Turnquist, B.; Rukwied, R.; Ringkamp, M.; Schmelz, M., 2012: The differential effects of two sodium channel modulators on the conductive properties of C-fibers in pig skin in vivo. Axonal sodium channels are attractive targets for chronic pain treatment, and recent evidence suggests that specific targeting of the slow inactivation of sodium channels (NaV) might exert analgesic effects. Using a human-like animal model, the pi...