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Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage


, : Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage. Journal of the Science of Food and Agriculture 93(7): 1633-1640

Chinese sturgeon (Acipenser sinensis), a rare species, is an important fishery resource in China. To save this species from overfishing, damming and pollution, artificial propagation has been developed rapidly in recent years. However, the cartilage from Chinese sturgeon, which contains an abundance of chondroitin sulfate (CS), is currently discarded as solid waste after consumption of the fish. The aim of this study was to investigate the extraction, purification and characterisation of CS from Chinese sturgeon cartilage. The optimal extraction parameters were a ratio of aqueous NaOH to cartilage powder of 9.2, a concentration of aqueous NaOH of 4.4% and an extraction time of 3.9 h. Under these optimal conditions the yield of crude CS from Chinese sturgeon cartilage was 26.51%, in agreement with the model prediction (26.54%). Purification by DEAE-52 cellulose and Sephadex G-100 column chromatography yielded a single fraction, CS-11. Its characterisation indicated that CS-11 was consistent with the polysaccharide backbone structure -4GlcAβ-3GalNβ- and was present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate. The results of this study provide a basis for promoting the utilisation of Chinese sturgeon resources and are significant for the development and utilisation of CS from Chinese sturgeon cartilage in the food industry.

US$19.90

PMID: 23288809

DOI: 10.1002/jsfa.5937


Other references

Anonymous, 2012: Nursing students’ perceptions on image of nursing a literature review. This paper highlights findings from a literature search to examine the nursing students perceptions of nursing and the review further focused on whether these views change during their nursing studies and impact workplace preferences. Unless attit...

S.E.D., 1991: Response. The author responds to comments made by F. Ruttner [Journal of Apicultural Research (1991) 30 (2) 113-114] concerning his [Moritz's] assessment of attempts by German bee breeders to replace the Apis mellifera mellifera population with A. m. c...

Vail J.W.; Barnard J.P., 1986: Reclamation of secondary sewage effluent by reverse osmosis a pilot plant study. Locally developed tubular reverse-osmosis (RO) membrane technology, incorporated into a novel and inexpensive support module, is being used in a 40 m3/d pilot plant to purify a filtered secondary sewage effluent from an activated sludge treatment...

Breci, L.A.; Tabb, D.L.; Yates, J.R.; Wysocki, V.H., 2003: Cleavage N-terminal to proline: analysis of a database of peptide tandem mass spectra. Fragmentation at the Xxx-Pro bond was analyzed for a group of peptide mass spectra that were acquired in a Finnigan ion trap mass spectrometer and were generated from proteins digested by enzymes and identified by the Sequest algorithm. Cleavage w...

Koschel, M.Jo., 2002: Where there's smoke, there may be cyanide. American Journal of Nursing 102(8): 39-42

Barrie, H.J., 1946: Pigmentary changes in familial non-haemolytic jaundice. A case of familial non-hemolytic jaundice is reported in which stercobilin excretion was persistently subnormal over a period of 34 days. The relationship of sterco- bilin excretion to changes in the blood bilirubin in this case suggested that the...

Woehrer W.; Roehr M., 1981: Regulatory aspects of bakers yeast saccharomyces cerevisiae metabolism in aerobic fed batch cultures. A highly instrumented computer-coupled bioreactor is used to investigate metabolic changes of S. cerevisiae in aerobic fed-batch systems which are generally applied in bakers' yeast manufacture. The 4 types of metabolism (oxidation of glucose...

Uchida, D.; Arimura, A.; Somogyvari Vigh, A.; Shioda, S.; Banks, W.A., 1996: Prevention of ischemia-induced death of hippocampal neurons by pituitary adenylate cyclase activating polypeptide. Because neurons in the CA1 region of the hippocampus are vulnerable to forebrain ischemia, this model has been used for evaluating neuroprotective agents. We evaluated the 38-amino-acid variant of pituitary adenylate cyclase activating polypeptide...

Kendrick, T.S.; Lipscombe, R.J.; Bogoyevitch, M.A., 2000: The use of cell-permeable peptides to identify proteins interacting with phosphorylated tyrosine 763 of the granulocyte colony stimulating factor receptor. Biochemical Society Transactions 28(5): A461, October

Teschner M.; Bahner U.; Heidland A., 1987: Preventing the progression of chronic renal insufficiency. Deutsche Medizinische Wochenschrift 112(9): 353-358