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Flagella overexpression attenuates Salmonella pathogenesis


, : Flagella overexpression attenuates Salmonella pathogenesis. Plos One 7(10): E46828-E46828

Flagella are cell surface appendages involved in a number of bacterial behaviors, such as motility, biofilm formation, and chemotaxis. Despite these important functions, flagella can pose a liability to a bacterium when serving as potent immunogens resulting in the stimulation of the innate and adaptive immune systems. Previous work showing appendage overexpression, referred to as attenuating gene expression (AGE), was found to enfeeble wild-type Salmonella. Thus, this approach was adapted to discern whether flagella overexpression could induce similar attenuation. To test its feasibility, flagellar filament subunit FliC and flagellar regulon master regulator FlhDC were overexpressed in Salmonella enterica serovar Typhimurium wild-type strain H71. The results show that the expression of either FliC or FlhDC alone, and co-expression of the two, significantly attenuates Salmonella. The flagellated bacilli were unable to replicate within macrophages and thus were not lethal to mice. In-depth investigation suggests that flagellum-mediated AGE was due to the disruptive effects of flagella on the bacterial membrane, resulting in heightened susceptibilities to hydrogen peroxide and bile. Furthermore, flagellum-attenuated Salmonella elicited elevated immune responses to Salmonella presumably via FliC's adjuvant effect and conferred robust protection against wild-type Salmonella challenge.

US$19.90

PMID: 23056473

DOI: 10.1371/journal.pone.0046828


Other references

Bakker, J.F.; Ricking, M., 1996: HCB in the EMS estuary. Abstracts of Papers American Chemical Society 212(1-2): ENVR 135

Hillstrom, J., 1982: Lilacs Ornamental horticulture. American horticulturist 61(4): 26-27

Rasheed, N.H.; Shannan, A.; Monofali, A.H., 2003: View and intents of AAAID in sugar crops production, industry and trade in the Arab Region. This presentation addresses: (1) the establishment, objectives, and areas of investment activity of the Arab Authority for Agricultural Investment and Development (AAAID); (2) sugar production, consumption, and supply balance in the Arab countries...

Mcclay E.F.; Albright K.; Jones J.; Christen R.; Howell S.B., 1992: Modulation of cisplatin ddp resistance by tamoxifen tam in human malignant melanoma. Proceedings of the American Association for Cancer Research Annual Meeting 33: 536

Ozmeriç, A.; Alemdaroğlu, K.Bahadır.; Aydoğan, N.Hürriyet., 2014: Treatment for cartilage injuries of the knee with a new treatment algorithm. Treatment of articular cartilage injuries to the knee remains a considerable challenge today. Current procedures succeed in providing relief of symptoms, however damaged articular tissue is not replaced with new tissue of the same biomechanical pr...

Baggi, G.; Bernasconi, S.; Zangrossi, M., 2005: 3-chloro-, 2,3- and 3,5-dichlorobenzoate co-metabolism in a 2-chlorobenzoate-degrading consortium: role of 3,5-dichlorobenzoate as antagonist of 2-chlorobenzoate degradation. Metabolism and co-metabolism of chlorobenzoates. A study was made of the metabolic and co-metabolic intermediates of 2- and 3-chlorobenzoate, 2,3- and 3,5-dichlorobenzoate to elucidate the mechanism(s) involved in the negative effects observed on the growth of a chlorobenzoate-degrading microbia...

Spady, D.K.; Bilheimer, D.W.; Dietschy, J.M., 1983: Rates of receptor-dependent and -independent low density lipoprotein uptake in the hamster. By using a constant infusion technique in the hamster, rates of uptake of [14C]sucrose-labelled hamster low-density lipoprotein (hamLDL) and methylated hamster LDL (Me-hamLDL) were directly measured in 15 tissues. The magnitude of LDL receptor-dep...

Broecker W.S.; Andree M.; Bonani G.; Wolfli W.; Oeschger H.; Klas M., 1988: Can the greenland climatic jumps be identified in records from ocean and land. Sharp jumps in climate punctuate the records from borings in the Greenland ice cap during the time interval 60,000 to about 20,000 yr ago. Rapid fluctuations are also seen in foraminifera records for cores from the northern Atlantic and in a polle...

Pisko Dubienski Z.A.; Baird R.J.; Wilson D.R., 1974: Noninvasive assessment of aorto coronary saphenous vein bypass graft patency by using directional doppler. Circulation Supplement 50(4-3): III137

Anonymous, 1978: Potatoes - report on a consumer survey 1977-78. This report is the third in a series of independent surveys undertaken amongst housewives and commissioned by the Potato Marketing Board. The survey took place in two phases, the first during November-December 1977 and the second during February-A...