geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Flagella proteins contribute to the production of outer membrane vesicles from Escherichia coli W3110


, : Flagella proteins contribute to the production of outer membrane vesicles from Escherichia coli W3110. Biochemical and Biophysical Research Communications 441(1): 151-156

Gram-negative bacteria, including Escherichia coli, release outer membrane vesicles (OMVs) that are derived from the bacterial outer membrane. OMVs contribute to bacterial cell-cell communications and host-microbe interactions by delivering components to locations outside the bacterial cell. In order to explore the molecular machinery involved in OMV biogenesis, the role of a major OMV protein was examined in the production of OMVs from E. coli W3110, which is a widely used standard E. coli K-12 strain. In addition to OmpC and OmpA, which are used as marker proteins for OMVs, an analysis of E. coli W3110 OMVs revealed that they also contain abundant levels of FliC, which is also known as flagellin. A membrane-impermeable biotin-labeling reagent did not label FliC in intact OMVs, but labeled FliC in sonically disrupted OMVs, suggesting that FliC is localized in the lumen of OMV. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. In addition, an E. coli W3110-derived strain with a null-mutation in flgK, which encodes flagellar hook-associated protein that is essential along with FliC for flagella synthesis, also produced fewer OMVs than the parental strain. Taken together, these results indicate that the ability to form flagella, including the synthesis of flagella proteins, affects the production of E. coli W3110 OMVs.

US$19.90

PMID: 24134841

DOI: 10.1016/j.bbrc.2013.10.022


Other references

Corso, A.; Zappasodi, P.; Barbarano, L.; Petrucci, M.Teresa.; Palumbo, A.; Caravita, T.; Mangiacavalli, S.; Cafro, A.Maria.; Varettoni, M.; Gay, F.; Morra, E.; Lazzarino, M., 2009: Long-term outcome in relapsed and refractory multiple myeloma treated with thalidomide. Balancing efficacy and side-effects. A total of 303 MM patients were retrospectively reviewed to evaluate long-term efficacy and toxicity of thalidomide alone or in combination with steroids. Overall response rate was 57% (CR/VGPR 12%). Median TTP, PFS and OS were 13.4 months, 20.6 m...

Moser, W.; Schindler, C.; Keiser, J., 2017: Efficacy of recommended drugs against soil transmitted helminths: systematic review and network meta-analysis. <b>Objective</b> To evaluate efficacies of anthelmintic drugs against soil transmitted helminths in terms of cure rates and egg reduction rates.<b>Design</b> Systematic review and network meta-analysis.<b>Data Sourc...

Wang, Q.; Seeman, E., 2008: Skeletal growth and peak bone strength. Bone size, shape and internal architecture, and not just bone mass, account for differences in bone strength between individuals, sexes and races. The differences in bone morphology in old age - whether an individual's bone size and mass occu...

Beklemishev, KV.; Pantyulin, AN.; Semenova, NL., 1980: Biological structure of the White Sea 2 New data on the vertical zonation of the White Sea. Trudy Belomorskoi Biologicheskoi Stantsii Moskovskogo Gosudarstvennogo Universiteta: 5: 8-19

Krkov, K., 1965: The sand beetle (Opatrum sabulosum L., fam. Tenebrionidae)-its biology and control. The adult overwinters in the soil of pastures, hedges and roads at a depth of 5-10cm under stones and trees, and occasionally in arable land at 15-40cm. The adult leaves the soil when the average daily air temperature is 8.8-15.5 degrees C, the re...

Møller, M., 1999: Introduction to mammalian pineal innervation. Microscopy Research and Technique 46(4-5): 235-238

Dworzecki, M., 2011: Diseases in the ghetto. American Ose Review 4(1): 18-25

Elshafei A.M.; Vega J.L.; Klasson K.T.; Clausen E.C.; Gaddy J.L., 1990: Cellulase and hemicellulase formation by fungi using corn stover as the substrate. The production of cellulase and hemicellulase enzymes by 16 fungi are compared using 20-mesh untreated corn stover as the substrate. The experimental results show that several fungi have potential for use in producing cellulase and hemicellulase d...

Norkus A.V.; Danis Y.K.; Dargis V.V.; Ostrauskas R.V.; Shachkute A.A.; Misyavichene I.S., 1987: Early diagnosis of diabetes mellitus in the rural population. Sovetskoe Zdravookhranenie (4): 15-18

Yokota, Y.; Kishida, T.; Esaki, K.; Kawamata, J., 1976: Anti tumor effects of interferon on transplanted tumors in congenitally athymic nude mice. The effects of mouse and human interferons on mouse (sarcoma 130) and human (HeLa) tumor cells transplanted into nude mice were investigated. The effects of these interferons on transplanted mouse tumor cells in ordinary mice were studied for comp...