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Functional roles for the GerE-family carboxyl-terminal domains of nitrate response regulators NarL and NarP of Escherichia coli K-12


, : Functional roles for the GerE-family carboxyl-terminal domains of nitrate response regulators NarL and NarP of Escherichia coli K-12. Microbiology 156(Pt 10): 2933-2943

NarL and NarP are paralogous response regulators that control anaerobic gene expression in response to the favoured electron acceptors nitrate and nitrite. Their DNA-binding carboxyl termini are in the widespread GerE-LuxR-FixJ subfamily of tetrahelical helix-turn-helix domains. Previous biochemical and crystallographic studies with NarL suggest that dimerization and DNA binding by the carboxyl-terminal domain (CTD) is inhibited by the unphosphorylated amino-terminal receiver domain. We report here that NarL-CTD and NarP-CTD, liberated from their receiver domains, activated transcription in vivo from the class II napF and yeaR operon control regions, but failed to activate from the class I narG and fdnG operon control regions. Alanine substitutions were made to examine requirements for residues in the NarL DNA recognition helix. Substitutions for Val-189 and Arg-192 blocked DNA binding as assayed both in vivo and in vitro, whereas substitution for Arg-188 had a strong effect only in vivo. Similar results were obtained with the corresponding residues in NarP. Finally, Ala substitutions identified residues within the NarL CTD as important for transcription activation. Overall, results are congruent with those obtained for other GerE-family members, including GerE, TraR, LuxR and FixJ.

US$19.90

PMID: 20634237

DOI: 10.1099/mic.0.040469-0


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Beasley, R.P.; Shiao, I.S.; Wu, T.C.; Hwang, L.Y., 1982: Hepatoma in an HBsAg carrier--seven years after perinatal infection. Journal of Pediatrics 101(1): 83-84

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Rosen J.M.; Dale T.; Gavigan S.; Buhler T., 1991: Developmental and hormonal regulation of mammary gland gene expression. Journal of Cellular Biochemistry Supplement (15 PART B): 232

Breburda, C.S.; Griffin, B.P.; Pu, M.; Scalia, G.M.; Thomas, J.D., 1997: Comparison of three-dimensional echo and proximal flow convergence method for quantification of mitral regurgitation. Journal of the American College of Cardiology 29(2 SUPPL A): 174A

Takemura, T.; Rom, W.N.; Ferrans, V.J.; Crystal, R.G., 1989: Morphologic characterization of alveolar macrophages from subjects with occupational exposure to inorganic particles. Alveolar macrophages recovered by bronchoalveolar lavage from 43 nonsmoking or greater than 5-yr ex-smoking subjects with occupational exposure to inorganic particles (asbestos, n 1/2 19; silica, n 1/2 10; coal, n 1/2 14) were evaluated by light m...

Sjödin, C., 2005: Abolish compulsory referrals and raise the annual health care fee limit. Lakartidningen 101(49): 4053-4053