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Further evaluation of Somah: long-term preservation, temperature effect, and prevention of ischemia-reperfusion injury in rat hearts harvested after cardiocirculatory death


, : Further evaluation of Somah: long-term preservation, temperature effect, and prevention of ischemia-reperfusion injury in rat hearts harvested after cardiocirculatory death. Transplantation Proceedings 45(9): 3192-3197

To identify and evaluate the ideal temperature for long-term storage of hearts from donation after cardiocirculatory death, in the novel organ preservation solution Somah. DCD hearts from Sprague-Dawley rats were harvested after 30 minutes of euthanasia, preserved in Somah at 4°C, 10°C, 21°C, or 37°C for 24 hours and then reperfused with blood:Somah (3:1) perfusate at 37°C for 30 minutes. Myocardial biopsies were taken during storage and before and after reperfusion to assess the structural and functional viability of tissue using multiphoton imaging, biochemistry, and immunofluorescence. Myocyte viability, determined by Live-Dead and esterase assays, was similar at 4°C, 10°C, and 21°C (193, 198 and 217 normalized fluorescence counts [NFC]) with a significant decrease at 37°C (131 NFC). Upon reperfusion, esterase activity was enhanced in DCD hearts stored in Somah at 21°C but noticeably decreased at all other temperatures. High-energy adenosine triphosphate/creatine phosphate (ATP/CP) syntheses and the expression of structural/contractile proteins was well preserved at 21°C, both after 24-hour storage and upon reperfusion. In contrast, hearts stored at all other temperatures demonstrated variable degenerative changes, loss of protein expression, and/or deranged ATP/CP synthesis after 24 hours of storage and/or upon reperfusion. The robust maintenance of structural/functional integrity of cardiac tissue and the preservation of protein expression and cellular energy metabolism in DCD hearts after long-term preservation at subnormothermic temperature suggests that 21°C is ideal for long-term storage of DCD hearts in Somah solution.

US$19.90

PMID: 24182783

DOI: 10.1016/j.transproceed.2013.06.006


Other references

Gupta, R.; Uretsky, B.F., 2005: Gadodiamide-based coronary angiography in a patient with severe renal insufficiency. The risk of contrast-induced nephropathy (CIN) is extremely high in patients with preexisting renal insufficiency. Gadolinium-based coronary angiography has been proposed as an approach to prevent CIN in this high risk subgroup. We report the use...

Zhao Liang; Zheng Tianyu; Chen Ling; Tang Qunshu, 2007: Shear wave splitting in eastern and central China; implications for upper mantle deformation beneath continental margin. Eastern China, located at the east margin of the Eurasia plate, is an ideal place to study the deformation pattern of upper mantle beneath continental margin. We investigated the upper mantle anisotropy beneath eastern China by applying teleseismi...

Hamilton, C.W.; Bigner, S.H.; Wells, S.A.; Johnston, W.W., 1983: Metastatic medullary thyroid carcinoma in sputum. A light and electron microscopic study. Medullary thyroid carcinoma (MTC) was diagnosed in a 43-year-old male by light microscopy, electron microscopy and immunohistochemistry. Five years after thyroidectomy, malignant cells with the typical cytologic and electron microscopic features o...

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Maroof M.M.M., 1995: Gems and gemmology in Sri Lanka; the early history. The Australian Gemmologist 19(4): 169-174

Chism G.W.; Huang A.E.; Marshall J.A., 1979: Sensitive assay for proteases in sterile milk. A sensitive assay for protease activity based on the reaction of primary amino groups of trichloroacetic acid-soluble peptides and amino acids with fluorescamine was applied to sterile milk. The assay was linear in the range of 2-50 nmol of amino...

Doran, D.M.L., 1950: Science and physical medicine. PhysioTherapy 36(8): 158-160

Bradley, S.; Browning, N., 2013: Foreword. Ultramicroscopy 127: 1-1

Chepurnykh, G.I.; Mannapov, F.K., 1963: The growth and development of guinea pig young at different temperatures. When guinea pigs are kept continuously in the open air and their ration increased by 10%, their fertility increases (they have litters of 2-5), the monthly gain in weight of the young increases (from 5.8 g indoors to 7.4-8.7 g in the open air in t...