geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin


, : Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Research 36(17): 5678-5694

DNA double-strand breaks (DSBs) are extremely dangerous lesions with severe consequences for cell survival and the maintenance of genomic stability. In higher eukaryotic cells, DSBs in chromatin promptly initiate the phosphorylation of the histone H2A variant, H2AX, at Serine 139 to generate gamma-H2AX. This phosphorylation event requires the activation of the phosphatidylinositol-3-OH-kinase-like family of protein kinases, DNA-PKcs, ATM, and ATR, and serves as a landing pad for the accumulation and retention of the central components of the signaling cascade initiated by DNA damage. Regions in chromatin with gamma-H2AX are conveniently detected by immunofluorescence microscopy and serve as beacons of DSBs. This has allowed the development of an assay that has proved particularly useful in the molecular analysis of the processing of DSBs. Here, we first review the role of gamma-H2AX in DNA damage response in the context of chromatin and discuss subsequently the use of this modification as a surrogate marker for mechanistic studies of DSB induction and processing. We conclude with a critical analysis of the strengths and weaknesses of the approach and present some interesting applications of the resulting methodology.

US$19.90

PMID: 18772227

DOI: 10.1093/nar/gkn550


Other references

Qiu, Z.; Yu, J.; Yan, P.; Wang, Z.; Wan, Q.; Yang, N., 2016: Electrochemical Grafting of Graphene Nano Platelets with Aryl Diazonium Salts. NlmCategory="UNASSIGNED">To vary interfacial properties, electrochemical grafting of graphene nano platelets (GNP) with 3,5-dichlorophenyl diazonium tetrafluoroborate (aryl-Cl) and 4-nitrobenzene diazonium tetrafluoroborate (aryl-NO&l...

Fernandes, P.H.; Hackfeld, L.C.; Kozekov, I.D.; Hodge, R.P.; Lloyd, R.Stephen., 2006: Synthesis and mutagenesis of the butadiene-derived N3 2'-deoxyuridine adducts. 1,3-Butadiene is a known carcinogen and mutagen that acts through a variety of metabolic intermediates that react with DNA, forming stable and unstable lesions on dG, dA, dC, and dT. The N3 2'-deoxyuridine adducts are a highly stable, stereoi...

Mandic, R.; Fackler, O.T.; Yu, H.; Luo, W.; Geyer, M.; Peterlin, B.M.tija, 1999: The role of NBP-1 in HIV/SIV Nef endocytosis. Molecular Biology of the Cell 10(SUPPL ): 220a, Nov

Sethi A.S.; Mor R.P.; Aggarwal G.C., 1985: A model to predict wheat emergence from soil temperature and soil moisture. The seedling emergence of 2 wheat varieties at various temperatures and soil moisture levels was evaluated. Mean emergence (over moistures and temperatures) was related to time through a Mitscherlich equation. A 2nd degree logistic growth model wa...

Haque, C.E.; Kolba, M.; Morton, P.; Quinn, N.P., 2002: Public involvement in the Red River Basin management decisions and preparedness for the next flood. The focus of this study is public participation in the water resource and associated hazards management decision-making processes. It explores the importance, feasibility, and effectiveness of public participation in the flood management, decision...

Suzuki Y.; Hayakawa S., 1989: A report on the conditions of agricultural meteorology in taiwan. Journal of Agricultural Meteorology 45(2): 111-115

Dongmei Yang; Guoyong Li; Shucun Sun, 2009: The effects of leaf size, leaf habit, and leaf form on leaf/stem relationships in plant twigs of temperate woody species. Journal of Vegetation Science 20(2): 359-366

Cesa-Bianchi, D., 1908: Alcune osservazioni sulla cellula interstiziale dellovaia. Monitore Zoologico Italiano, 79 258-263

Peterková, R., 1985: The common developmental origin and phylogenetic aspects of teeth, rugae palatinae, and fornix vestibuli oris in the mouse. Positional and temporal information is of fundamental importance in understanding the morphogenesis of dentition. In order to determine the fate of epithelial cells localized within specific epithelial thickened regions of the forming mouse maxill...

Mikhak, Z.; Luster, A.D., 2010: The emergence of basophils as antigen-presenting cells in Th2 inflammatory responses. Basophils gain prominence in Th2 inflammatory responses with the discovery that they function as antigen-presenting cells and are sufficient to drive Th2 cell differentiation.