geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gene transfer in the liver using recombinant adeno-associated virus


, : Gene transfer in the liver using recombinant adeno-associated virus. Current Protocols in Microbiology Chapter 14: Unit14d.6-Unit14d.6

Liver-directed gene transfer and gene therapy are rapidly gaining attention primarily because the liver is centrally involved in a variety of metabolic functions that are affected in various inherited disorders. Recombinant adeno-associated virus (rAAV) is a popular gene delivery vehicle for gene therapy, and intravenous delivery of some rAAV serotypes results in very efficient transduction in the liver. rAAV-mediated gene transfer to the liver can be used to create somatic transgenic animals or disease models for studying the function of various genes and miRNAs. The liver is the target tissue for gene therapy of many inborn metabolic diseases and may also be exploited as a "biofactory" for production of coagulation factors, insulin, growth hormones, and other non-hepatic proteins. Hence, efficient delivery of transgenes and small RNAs to the liver by rAAV vectors has been of long-standing interest to research scientists and clinicians alike. This unit describes methods for delivery of rAAV vectors by several injection routes, followed by a range of analytical methods for assessing the expression, activity, and effects of the transgene and its product.

US$19.90

PMID: 23686826

DOI: 10.1002/9780471729259.mc14d06s29


Other references

Balter, S.G.T.; Stokroos, R.J.; Akkermans, E.; Kingma, H., 2004: Habituation to galvanic vestibular stimulation for analysis of postural control abilities in gymnasts. The possible correlation between postural control abilities in gymnasts and the sensitivity for and the degree of short-term habituation to galvanic vestibular stimulation (GVS) was studied. Seven balance trained young girls (Dutch National Junior...

Anonymous, 2009: Abstracts of the 13th British Academic Conference in Otolaryngology and ENT Expo. July 8-10, 2009. Liverpool, United Kingdom. Clinical Otolaryngology 34 Suppl 1: 1-138

Jung H D.; Wolff F., 1980: Milkers eczema within the agricultural industry district of neubrandenburg east germany. There is an established increased tendency in milker's eczema within the agricultural industry district of Neubrandenburg, East Germany. During the last 5 yr, 58 contact eczema cases were observed within the territory, which were produced by...

Kent, G.A.; McKenzie, N.J., 2001: The distribution of torque in a three-roll mill. The widespread use of independent hydraulic drives on individual roll shafts has given factories the ability to measure, rather than assume, the distribution of torque in a 3-roll mill. This study summarizes previously published measurements of th...

Haarmann, M.; Lenz, W.; Petersen, D., 1975: Radiusaplasia with T thrombocytopenia. A genetic syndrome. Ergebnisse der Inneren Medizin und Kinderheilkunde 37: 57-106

Brueckmann, M.; Wolpert, C.; Bertsch, T.; Sueselbeck, T.; Jaramillo, C.; Neumaier, M.; Huhle, G.; Borggrefe, M.; Haase, K.K., 2003: Markers of inflammation, myocardial damage, and tissue healing after high-energy radiofrequency ablation of atrial arrhythmias. Journal of the American College of Cardiology 41(6 Supplement A): 132A, March 19

LeVan, K.M.; Goldberg, E., 1991: Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli. The cDNA encoding the C4 isoenzyme of lactate dehydrogenase (LDH-C4) was engineered for expression in Escherichia coli. The Ldh-c open reading frame was constructed as a cassette for production of the native protein. The modified Ldh-c cDNA was su...

Barendregt, M.; Dumoulin, S.O.; Rokers, B., 2015: Stereomotion scotomas occur after binocular combination. Stereomotion scotomas are a surprisingly common visual impairment that result in an observer's inability to accurately report the direction of an object's motion in depth in restricted parts of the visual field. In this study we investig...

Bush L.R.; Holahan M.A.; Kanovsky S.M.; Mellott M.J., 1989: Influence of txa 2 receptor antagonists in animal models of thrombosis and thrombolysis. Journal of Molecular & Cellular Cardiology 21(SUPPL 2): S194

Mattila, K.M.; Hiltunen, M.; Rinne, J.O.; Mannermaa, A.; Roytta, M.; Alafuzoff, I.; Laippala, P.; Soininen, H.; Lehtimaki, T., 2003: Absence of association between an intercellular adhesion molecule 1 gene E469K polymorphism and Alzheimer's disease in Finnish patients. Increased expression of intercellular adhesion molecule 1 (ICAM1), a protein known to contribute to inflammatory responses, has been detected in the brain tissue of patients with Alzheimer's disease (AD) and animals modelled to mimic AD or Pa...